19,635 results

1. pAAV-N-RAM-d2tTA-TRE-mKate2

   Plasmid

   #140275

   Purpose

   N-RAM, the Npas4-dependent reporter

   Depositor

   Yingxi Lin

   Article

   Sun et al Cell. 2020 Apr 16;181(2):410-423.e17. do

   Insert

   mKate2
   

   Use

   AAV

   Promoter

   TRE

   Available Since

   July 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-HIF1alpha-wt-pBabe-puro

   Plasmid

   #19365

   Depositor

   William Kaelin

   Article

   Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

   Insert

   Hypoxia inducible factor 1 alpha (HIF1A Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   wt human cDNA

   Available Since

   April 6, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. SPLICS PM-ER Long P2A

   Plasmid

   #164111

   Purpose

   Detect the long Plasma membrane-Endoplasmic reticulum contact

   Depositor

   Marisa Brini, Tito Calì

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split YFP PM-ER Long P2A
   

   Expression

   Mammalian

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAF0689 Ef1a-Pa01_attB-BFP acceptor

   Plasmid

   #193472

   Purpose

   Acceptor plasmid for 3 plasmid recombination assay

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Pa01_attB
   

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMK287 (mAID-Hygro)

   Plasmid

   #72825

   Purpose

   C-terminal tagging with mAID using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-Hygro
   

   Use

   CRISPR

   Tags

   mAID

   Expression

   Mammalian

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF6-Acly(S455A)

   Plasmid

   #70767

   Purpose

   Expresses phosphomutant mouse ATP-citrate lyase in mammalian cells

   Depositor

   Kathryn Wellen

   Article

   Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

   Insert

   ATP Citrate Lyase (Acly Mouse)
   

   Tags

   myc-epitope

   Expression

   Mammalian

   Mutation

   S455A

   Promoter

   EF1alpha

   Available Since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR_PGK

   Plasmid

   #79120

   Purpose

   Lentiviral vector for constitutive expression of genes (PGK promoter)

   Depositor

   Wendell Lim

   Article

   Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX304 CMV HA-HaloTag-FRB-sTurboID-high affinity (C)

   Plasmid

   #153005

   Purpose

   Expresses high-affinity split-TurboID C-terminal fragment in mammalian cells

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   sTurboID-high affinity (C) (G79)
   

   Use

   Lentiviral

   Tags

   FRB, HA, and HaloTag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. FH-TET3-pEF

   Plasmid

   #49446

   Purpose

   This plasmid expresses full-length human TET3.

   Depositor

   Anjana Rao

   Article

   Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

   Insert

   Tet methylcytosine dioxygenase 3 (TET3 Human)
   

   Tags

   Flag and HA

   Expression

   Mammalian

   Promoter

   EF-1alpha

   Available Since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET16B.Pfu

    Plasmid

    #12509

    Purpose

    Bacterial expression of Pfu DNA polymerase

    Depositor

    Sung-Hou Kim

    Article

    Goldman et al Acta Crystallogr D Biol Crystallogr. 199

    Insert

    Pfu DNA polymerase
    

    Expression

    Bacterial

    Mutation

    Y218H, Q462R, F533L, K559I and V683A (mutations w…

    Available Since

    Sept. 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCBh-NLS_dCas13d_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid

    #191797

    Purpose

    vector for encoding a human codon-optimized dead RfxCas13d (dCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

    Insert

    humanized dCas13d
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    R239A, H244A, R858A, H863A

    Promoter

    CBh, SV40, hU6

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC007 - pRFP constitutive expression

    Plasmid

    #79156

    Purpose

    RFP constitutively expressed

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

    Insert

    RFP
    

    Available Since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV R-CEPIA3mt

    Plasmid

    #140464

    Purpose

    Red fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

    Depositor

    Masamitsu Iino

    Article

    Kanemaru et al Sci Rep. 2020 Feb 18;10(1):2835. doi: 10

    Insert

    R-CEPIA3mt
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-fDIO-mCherry-WPREpA

    Plasmid

    #177328

    Purpose

    Control vector for Flp-dependent DREADD constructs

    Depositor

    Ulrik Gether

    Insert

    mCherry
    

    Use

    AAV; Flp-frt

    Expression

    Mammalian

    Promoter

    Human Synapsin-1

    Available Since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCardinal-Lifeact-7

    Plasmid

    #54663

    Purpose

    Localization: Actin, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Type

    Empty backbone

    Tags

    mCardinal-Lifeact

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. CaVα2δ1

    Plasmid

    #26575

    Depositor

    Diane Lipscombe

    Article

    Lin et al J Neurophysiol. 2004 Nov . 92(5):2820-30

    Insert

    Cacna2d1 (Cacna2d1 Rat)
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. TetO-FUW-OSKM

    Plasmid

    #20321

    Purpose

    Lentiviral plasmid for tet-inducible expression of mouse Oct4, Sox2, Klf4 and Myc for iPS cell generation

    Depositor

    Rudolf Jaenisch

    Article

    Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

    Insert

    Oct-4 (Pou5f1, Sox2, Klf4, Myc Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA GSK3 beta S9A pcDNA3

    Plasmid

    #14754

    Purpose

    Mammalian expression of human GSK3 beta constitutively active mutant with HA tag

    Depositor

    Jim Woodgett

    Article

    Stambolic et al Biochem J. 1994 Nov 1;303 ( Pt 3)(Pt 3):

    Insert

    GSK3 beta S9A (GSK3B Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S9A. Constitutively active mutant.

    Available Since

    April 12, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pET-11b_PcrA_M6_H93A

    Plasmid

    #194156

    Purpose

    Engineered PcrA_M6 helicase to support isothermal amplification method SHARP

    Depositor

    Taekjip Ha

    Article

    Gavrilov et al Nat Commun. 2022 Oct 23;13(1):6312. doi:

    Insert

    Engineered helicase PcrA_M6
    

    Tags

    6x His tag and FLAG tag

    Expression

    Bacterial

    Mutation

    H93A, C96A, N187C, C247A, L384V, L409C

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. TCRalpha_E2A_SP-SNAPf-TCRbeta_P2A_CD3e_T2A_CD3zeta

    Plasmid

    #89347

    Purpose

    2nd generation lentiviral vector which expresses SNAP tagged TCRbeta and other TCR subunits (Jurkat alleles) separated via 2A sequences

    Depositor

    Ron Vale

    Article

    Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

    Insert

    Jurkat TCRalpha/beta alleles and CD3e and CD3z
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    May 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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