12,323 results

1. pAL189

   Plasmid

   #22278

   Purpose

   Expresses Intersectin DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment

   Depositor

   Christopher Voigt

   Article

   Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

   Insert

   IntersectinDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgRNA AAVS1

   Plasmid

   #128119

   Purpose

   Co-expresses sgRNA AAVS1, SpyCas9 scaffold (F+E) and GFP (transfection marker)

   Depositor

   Dominik Niopek

   Article

   Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

   Insert

   sgRNA AAVS1 + GFP
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   RSV/U6

   Available Since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAV114

   Plasmid

   #63181

   Purpose

   pRS414 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSABEn

   Plasmid

   #133969

   Purpose

   Expresses ABEn CRISPR base editor and gRNA scaffold in Streptomyces

   Depositor

   Yuhui Sun

   Article

   Zhong et al bioRxiv 630137

   Insert

   ABEn
   

   Use

   CRISPR and Synthetic Biology

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEG302 22aa SunTag NtDRMcd (no NLS) SUP

   Plasmid

   #115490

   Purpose

   CRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to the SUPERMAN locus with two guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g2_U6_g1_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. LSB-hsa-miR-186-5p

   Plasmid

   #103289

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-186-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-186-5p target (MIR186 Human)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSilencer2.1-U6-bc339

   Plasmid

   #190848

   Purpose

   For mammalian expression of bc339, the RNA aptamer of β-catenin. Expression is driven by a U6 promoter.

   Depositor

   Gerald Hart

   Article

   Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

   Insert

   bc339 (an RNA aptamer of β-catenin)
   

   Use

   Affinity Reagent/ Antibody and Synthetic Biology

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 9, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pSilencer2.1-U6-AP3

   Plasmid

   #190826

   Purpose

   For mammalian expression of AP3, the RNA aptamer of GFP. Expression is driven by a U6 promoter.

   Depositor

   Gerald Hart

   Article

   Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

   Insert

   AP3 (an RNA aptamer of GFP)
   

   Use

   Affinity Reagent/ Antibody and Synthetic Biology

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 9, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pL0_EF-H4

   Plasmid

   #202178

   Purpose

   Level 0 plasmid containing a terminator sequence from gene 34971 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   H4 terminator
   

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 28, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pL0_EF-fcpA

    Plasmid

    #202164

    Purpose

    Level 0 plasmid containing a terminator sequence from gene 18049 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    fcpA terminator
    

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pL0_AC-H4

    Plasmid

    #202122

    Purpose

    Level 0 plasmid containing the promoter from gene 34971 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    H4 promoter
    

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pL0_AC-P49

    Plasmid

    #202120

    Purpose

    Level 0 plasmid containing the promoter from gene 49202 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    P49 promoter
    

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. LLP737_L2pV1_1I_Act2_FRT_OCS_Act2_Flp_lacZ

    Plasmid

    #192380

    Purpose

    To test if the Act2 promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pC0.286

    Plasmid

    #119627

    Purpose

    Level 0 Part. CDS

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    AbRChl
    

    Use

    Synthetic Biology

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXLK_aFb

    Plasmid

    #114230

    Purpose

    POC12368, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type a-b

    Depositor

    Christopher A. O'Callaghan

    Article

    Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

    Insert

    LacZalpha
    

    Use

    Synthetic Biology

    Available Since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. LSB-hsa-miR-485-3p

    Plasmid

    #103553

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-485-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-485-3p target (MIR485 Human)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-EF1a-CCre-MBP4-WPRE

    Plasmid

    #193919

    Purpose

    Expresses one of the components of Cre-DOR_N5C4 (RFP-dependent Cre)

    Depositor

    Tatsushi Onaka

    Article

    Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

    Insert

    CCre-MBP4
    

    Use

    AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1a-NCre-MBP5-WPRE

    Plasmid

    #193918

    Purpose

    Expresses one of the components of Cre-DOR_N5C4 (RFP-dependent Cre)

    Depositor

    Tatsushi Onaka

    Article

    Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

    Insert

    NCre-MBP5
    

    Use

    AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. B. fragilis 16S toehold switch sensor

    Plasmid

    #110696

    Purpose

    Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

    Depositor

    James Collins

    Article

    Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

    Insert

    B. fragilis 16S toehold switch sensor
    

    Use

    Synthetic Biology

    Promoter

    T7

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUPD1:Rb7-MAR

    Plasmid

    #106212

    Purpose

    Provides N.tabacum RB7 matrix attachment region as a level 0 GoldenBraid part

    Depositor

    Tomáš Moravec

    Insert

    N.tabacum RB7 matrix attachment region
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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