We narrowed to 4,074 results for: p2a

1. AAV-CMV-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

   Plasmid

   #177365

   Purpose

   AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Inserts

   tdTomato
   Chimeric guide for SaCas9
   

   Use

   AAV

   Tags

   P2A and a tetracycline-controlled transactivator …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6 promoter

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgRNA1_Cas9-hGem_Smc4

   Plasmid

   #217667

   Purpose

   expresses Cas9-hGem and guideRNA for tagging of SMC4

   Depositor

   Daniel Gerlich

   Article

   Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

   Insert

   sgRNA1 targeting SMC4 C-terminal (SMC4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-APOBEC1-YTHmut-EGFP

   Plasmid

   #209323

   Purpose

   AAV packaging vector containing a APOBEC1-YTHmut expression cassette, a P2A-EGFP expression cassette.

   Depositor

   Magdalena Koziol

   Article

   Feng et al Genome Res. 2024 Jul 23;34(6):822-836. d

   Insert

   APOBEC1--YTHmut-HA
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   YTH domain lacks AA 385-409 comprising the m6A-bi…

   Promoter

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-EGFP-APOBEC1-YTHmut

   Plasmid

   #209320

   Purpose

   AAV packaging vector containing a EGFP-P2A expression cassette, APOBEC1-YTHmut cassette.

   Depositor

   Magdalena Koziol

   Article

   Feng et al Genome Res. 2024 Jul 23;34(6):822-836. d

   Insert

   APOBEC1-YTHmut-HA
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   YTH domain lacks AA 385-409 comprising the m6A-bi…

   Promoter

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. DRD2-V2tail-TevN-BLITz-TetR-FKBP

   Plasmid

   #210514

   Purpose

   expresses DRD2-V2tail-TevN-BLITz-TetR-FKBP component in mammalian cells

   Depositor

   Dongmin Lee

   Article

   Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

   Insert

   DRD2-V2tail-TevN-BLITz-TetR-FKBP
   

   Use

   Tags

   HA signal FALG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Sniper2P

   Plasmid

   #193857

   Purpose

   Expresses human codon-optimized Sniper2P and blasticidin resistance: EFS promoter-Sniper2P-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Chem Biol. 2023 Mar 9. doi: 10.1038/

   Insert

   Sniper2L
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   E1007L

   Promoter

   EFS

   Available since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. S113

   Plasmid

   #174551

   Purpose

   Constitutive PhCMV-driven mammalian expression vector of CDH-3-TF cassette (PhCMV-Gal4-mdm2-P2A-LD6-Rel65-pA)

   Depositor

   Bruno Correia

   Article

   Shui et al Nat Commun. 2021 Oct 1;12(1):5754. doi:

   Insert

   mdm2 and designed protein LD6 (MDM2 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUFlip-floxed2A-KalTA4-; gcry1:BFP -2

   Plasmid

   #173891

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-KalTA4; gcry1: BFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJM621 EF1α TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-DsDed-ZF1 in TUPV3

   Plasmid

   #161572

   Purpose

   Constitutive expression of TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-DsDed-ZF1 under the EF1α promoter

   Depositor

   Joshua Leonard

   Article

   Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

   Insert

   TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-DsDed-ZF1
   

   Use

   Synthetic Biology

   Tags

   3x-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSN29

    Plasmid

    #100114

    Purpose

    expresses a chimeric cMYC-repressor fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCerulean-P2A-cMYC fused to full length HP1 alpha (MYC, CBX5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSN17

    Plasmid

    #100104

    Purpose

    expresses a chimeric OCT4-repressor fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    vexGFP-P2A-OCT4 fused to full length HP1 alpha (POU5F1, CBX5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. RV-cag-Dio-Kif5c-2A-tdt

    Plasmid

    #87668

    Purpose

    retroviral vector, conditional expression membrane Tdtomato and Kif5Cdelta560

    Depositor

    Oscar Marin

    Article

    Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

    Insert

    Kif5C-P2A-mTdt (Kif5c Rat)
    

    Use

    Retroviral

    Tags

    palmitylation

    Expression

    Mutation

    motor domain aa1-560 present, V40A, E125V (see de…

    Promoter

    CAG

    Available since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPKm-240

    Plasmid

    #90510

    Purpose

    pSIN - EF-1alpha - Cyto -sFd - P2A - Cyto - sFNR, vector encoding for cytoplasmic-tagged Synechococcus sp. Heme Oxigenase-1 (HO1) and Phycocyanobilin:ferredoxin oxireductase (PcyA), under EF-1alpha promoter

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    cyto-sFD, cyto-sFNR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-SET-FLAG-HA

    Plasmid

    #24998

    Depositor

    Judy Lieberman

    Article

    Yan et al PLoS Pathog. 2009 Mar . 5(3):e1000327.

    Insert

    SET (SET Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-SETin-FLAG-HA

    Plasmid

    #24999

    Depositor

    Judy Lieberman

    Article

    Yan et al PLoS Pathog. 2009 Mar . 5(3):e1000327.

    Insert

    SET (SET Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    silent mutations introduced to prevent siRNA targ…

    Promoter

    Available since

    Aug. 9, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pUb FLAG-wdb

    Plasmid

    #195069

    Purpose

    Expresses FLAG-tagged Drosophila wdb protein

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    widerborst (wdb Fly)
    

    Use

    Tags

    3xFLAG

    Expression

    Insect

    Mutation

    Promoter

    Ubi-p63e

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR-PPP2R2B

    Plasmid

    #16181

    Depositor

    Huda Zoghbi

    Article

    Lim et al Cell. 2006 May 19. 125(4):801-14.

    Insert

    PP2A regulatory subunit B, beta isoform (PPP2R2B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pMKO.1 GFP B55 alpha shRNA 3-1

    Plasmid

    #10679

    Depositor

    William Hahn

    Article

    Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

    Insert

    PP2A B55 alpha shRNA (PPP2R2A Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pMKO.1 GFP B56 gamma3 shRNA 14-1

    Plasmid

    #10681

    Depositor

    William Hahn

    Article

    Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

    Insert

    PP2A B56 gamma3 shRNA (PPP2R5C Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pMKO.1 GFP B56 alpha shRNA 6-3

    Plasmid

    #10680

    Depositor

    William Hahn

    Article

    Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

    Insert

    PP2A B56 alpha shRNA (PPP2R5A Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only