We narrowed to 4,377 results for: p2a

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pC034 - LwCas13a-msfGFP-2A-Blast

Plasmid

#91924

Purpose

Expresses active LwCas13a for mammalian RNA targeting with Blasticidin selection marker

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LwCas13a

Use

CRISPR and Lentiviral

Tags

NLS and msfGFP-NLS-3xHA, P2A, Blasticidin

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pEIF3D-NtermDel-sgEIF3D

Plasmid

#222123

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

S161 truncation

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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AAVS1_Cas9-hGem_gRNA2

Plasmid

#217659

Purpose

expresses Cas9-hGem and guideRNA targeting the human AAVS1 safe harbour locus

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

sgRNA2 targeting the human AAVS1 safe harbour locus

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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LRG2.1_Neo

Plasmid

#125593

Purpose

Lentiviral expression of sgRNA with GFP and neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter for sgRNA expression and EFS promoter…

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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Cas9-2A-Cre

Plasmid

#68468

Purpose

co-expressing Cas9 and Cre recombinase

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Insert

Cas9 and Cre recombinase

Use

CRISPR

Tags

P2A-Cre-Myc

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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evoCas9

Plasmid

#138558

Purpose

Expresses human codon-optimized evolved SpCas9 and blasticidin resistance: EFS promoter-evoCas9-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

evoCas9

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

M495V, Y515N, K526E, R661Q

Promoter

EFS

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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TRE-SEAP

Plasmid

#210511

Purpose

SEAP reporter vector for LAUNCHER system

Depositor

Dongmin Lee

Article

Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

Insert

secreted alkaline phosphatase

Expression

Mammalian

Promoter

TetO

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pHD-pYtag(Clover)-ScarlessDsRed

Plasmid

#244931

Purpose

Vector containing a Clover-based pYtag biosensor for CRISPR insertion into Drosophila RTKs, with a DsRed marker.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

Clover-tagged pYtag system (3xITAM P2A Clover-ZtSH2)

Use

CRISPR

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSN16

Plasmid

#100103

Purpose

expresses a chimeric OCT4-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

vexGFP-P2A-OCT4 fused to a 'strong' VP16 domain (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-DRD2-V2tail-TevN-BLITz1-TetR-VP16

Plasmid

#89870

Purpose

expresses DRD2-iTango component in mammalian cells

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

DRD2-V2tail-TevN-BLITz1-TetR-VP16

Expression

Mammalian

Mutation

short isoform of DRD2 (deletion of 242-270)

Promoter

CMV

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-HNF4A-FOXA2-FOXA3

Plasmid

#149724

Purpose

expressing three liver promoting transcription factors (HNF4A, FOXA2, FOXA3)

Depositor

Baisong Lu

Article

Lu et al Biomaterials. 2021 Feb;269:120668. doi:

Insert

HNF4A-FOXA2-FOXA3

Use

Lentiviral

Tags

P2A, T2A

Promoter

CMV

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pPBT/EFS-Cas9-PuroTk

Plasmid

#170542

Purpose

Expresses spCas9 with a P2A junction to the puromycin resistance gene directly fused to the thymidine kinase

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Insert

PuroTK

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pFETCh_AHR

Plasmid

#101071

Purpose

Donor vector for 3' FLAG tag of human AHR

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

AHR homology arms (AHR Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCH4 (denAsCas12a-KRAB lenti)

Plasmid

#217331

Purpose

Lentiviral expression of denAsCas12a-KRAB

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

denAsCas12a

Use

CRISPR and Lentiviral

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Mutation

D908A/E174R/S542R/K548R

Promoter

UCOE-SFFV

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pFETCh_KLF9

Plasmid

#86286

Purpose

Donor vector for 3' FLAG tag of human KLF9

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

KLF9 homology arms (KLF9 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-NRCH-PE2-BSD

Plasmid

#191101

Purpose

Lentiviral expression plasmid of NRCH-PE prime editor with P2A-BSD marker

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

NRCH-PE2-BSD

Use

CRISPR and Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF-1a

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBM019

Plasmid

#128179

Purpose

Construct for generating stable Cas9-expressing Toxoplasma gondii

Depositor

Sebastian Lourido

Article

Markus et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003

Inserts

sgRNA#1
SpCas9
Chloramphenicol acetyltransferase

Use

CRISPR; Toxoplasma gondii expression

Tags

3X FLAG and Nuclear Localization Signal

Promoter

TUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pFETCh_KDM1A

Plasmid

#86260

Purpose

Donor vector for 3' FLAG tag of human KDM1A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

KDM1A homology arms (KDM1A Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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T/brachyury(2A-Dre)

Plasmid

#140761

Purpose

Targeting vector to T/brachyury gene locus for induction of P2A-fused Dre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F3) sequence.

Depositor

Hideyuki Okano

Article

Serizawa et al Development. 2019 Nov 4;146(21). pii: de

Insert

Dre

Use

Mouse Targeting

Promoter

Endogenous T/brachyury

Available Since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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SpCas9-NRTH

Plasmid

#198541

Purpose

Expresses human codon-optimized SpCas9-NRTH and blasticidin resistance: EFS promoter-SpCas9-NRTH-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NRTH

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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