We narrowed to 58,407 results for: tra

1. pHRE

   Plasmid

   #134909

   Purpose

   Overexpression of recombinant proteins in plants thanks to efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Test UTR combinations by swapping 5' and 3' UTRs.

   Depositor

   George Lomonossoff

   Article

   Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Plant

   Promoter

   double 35S

   Available Since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMD.RVG.CVS24-B2c

   Plasmid

   #19713

   Depositor

   Manfred Schubert

   Article

   Mentis et al J Neurosci Methods. 2006 Oct 30. 157(2):

   Insert

   Rabies virus CVS24-B2c glycoprotein
   

   Expression

   Mammalian

   Available Since

   Nov. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. CAG-FLEx(FRT)-TC

   Plasmid

   #67827

   Purpose

   Flp-dependent expression of TVA-mCherry fusion to mediate modifed rabies virus expression in cells

   Depositor

   Liqun Luo

   Article

   Schwarz et al Nature. 2015 Aug 6;524(7563):88-92. doi:

   Insert

   TVA-mCherry fusion
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRN119

   Plasmid

   #176884

   Purpose

   Encodes STM and WUS2 for ectopic meristem induction in plants

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Insert

   CmYLCV:Luc+:HSP, Nos::WUS2:pinII, and 35:STM:35S
   

   Expression

   Plant

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cas9 Null

   Plasmid

   #198727

   Purpose

   Expresses Cas9 without a mobility signal and the mRNA is not graft mobile. Induced by estradiol.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   Cas9
   

   Expression

   Plant

   Promoter

   pG10-90

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMD.RVG.CVS24-N2c

   Plasmid

   #19712

   Depositor

   Manfred Schubert

   Article

   Mentis et al J Neurosci Methods. 2006 Oct 30. 157(2):

   Insert

   Rabies virus CVS24-N2c glycoprotein
   

   Expression

   Mammalian

   Available Since

   Nov. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMW37: pRS426-R2Bm RNA

   Plasmid

   #209291

   Purpose

   Contains the R2Bm non-LTR retrotransposon 5'UTR and 3'UTR sequences

   Depositor

   Feng Zhang

   Article

   Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

   Insert

   R2Bm 5'UTR and 3'UTR
   

   Expression

   Bacterial and Yeast

   Promoter

   GAL1 GAPDH

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-3×FLAG-SLC35A2

   Plasmid

   #186283

   Purpose

   transient expression of N-terminal 3×FLAG tagged SLC35A2 isoform c/UGT1 in mammalian cells

   Depositor

   Somshuvra Mukhopadhyay

   Article

   Li et al J Biol Chem. 2021 Sep;297(3):101069. doi

   Insert

   SLC35A2 (SLC35A2 Human)
   

   Tags

   3×FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCEP4-SERT-myc

   Plasmid

   #107456

   Purpose

   Mammalian expression plasmid for codon optimized hSERT with internal MYC tag inserted in ECL2

   Depositor

   Erik Procko

   Article

   Young et al bioRxiv 2021.04.19.440442

   Insert

   Serotonin Transporter (SERT) (SLC6A4 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUB-MSP1D1

    Plasmid

    #161609

    Purpose

    Bacterial expression of mutated membrane scaffold protein ("ultra-bright" MSP1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

    Insert

    UD-MSP1D1
    

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    mutant of MSP1D1 ( Q138W, L181W, Y236W)

    Promoter

    T7

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. enz.083-NMT1

    Plasmid

    #154047

    Purpose

    Expresses NMT1 in E. coli

    Depositor

    Christopher Voigt

    Article

    Wallace et al Advanced Functional Materials 2020

    Insert

    NMT1 (N-myristoyltransferase 1) (NMT1 Human)
    

    Tags

    His, MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-LacI-NLS-VP16

    Plasmid

    #103836

    Purpose

    Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    EGFP-LacI-NLS-VP16
    

    Expression

    Mammalian

    Promoter

    SV2

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMS12: pDonor(AvCAST)

    Plasmid

    #168145

    Purpose

    AvCAST donor harboring non-functional cargo.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    AvCAST right end (RE)
    AvCAST left end (LE)
    

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-EF1a-NucGFP

    Plasmid

    #184706

    Purpose

    Lentiviral transfer plasmid for expression of NucGFP

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    NucGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. V2-MESA-35F-Tev

    Plasmid

    #84503

    Purpose

    MESA protease chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, and Tev protease

    Depositor

    Joshua Leonard

    Article

    Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

    Insert

    V2-MESA-35F-Tev
    

    Use

    AAV

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. V1-MESA-45F-Tev

    Plasmid

    #84501

    Purpose

    MESA protease chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, and Tev protease

    Depositor

    Joshua Leonard

    Article

    Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

    Insert

    V1-MESA-45F-Tev
    

    Use

    AAV

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMS29: pDonor(PmcCAST)

    Plasmid

    #168162

    Purpose

    PmcCAST donor harboring non-functional cargo.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    PmcCAST right end (RE)
    PmcCAST left end (LE)
    

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMS13: pTarget(AvCAST)

    Plasmid

    #168146

    Purpose

    Target plasmid containing AvPSP1 and attachement site for AvCAST.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    PAM for AvCAST + AvPSP1
    glmS
    

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. V2-MESA-35F-M-tTA

    Plasmid

    #84502

    Purpose

    MESA target chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, M cleavage sequence, and tTA

    Depositor

    Joshua Leonard

    Article

    Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

    Insert

    V2-MESA-35F-M-tTA
    

    Use

    AAV

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMARO

    Plasmid

    #178086

    Purpose

    Derivative of pMK2010 that contains tetracycline resistance insert between attL1 and attL2 flanked by EcorV. Suitable as control or backbone for library construction for in vivo Gateway reaction.

    Depositor

    Sören Abel

    Article

    Gillman et al Plasmid. 2021 Nov;118:102608. doi: 10.10

    Insert

    TcR (tetA/tetR)
    

    Expression

    Bacterial

    Mutation

    Tetracycline resistance cassette is flanked by Ec…

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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