Addgene

Showing: 3481 - 3500 of 4041 results

Results per page:

Sort results by:

pLVET-tTR-KRAB

Plasmid

#11644

Purpose

Tet-regulated (Tet-on) lentiviral vector for transgene (hEF-1alpha promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

hEF-1alpha, GFP, tTR-KRAB, Tet-on

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pBK-VQR-BE3

Plasmid

#85171

Purpose

Expresses VQR-BE3 in mammalian cells

Depositor

David Liu

Article

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

VQR-BE3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO-RB1-shRNA19

Plasmid

#25640

Purpose

3rd generation lentiviral vector

Depositor

Todd Waldman

Article

Michaud et al Cancer Res. 2010 Apr 15. 70(8):3228-38.

Insert

RB1-shRNA 19 (RB1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
PL552

Plasmid

#68407

Purpose

Donor empty vector with floxed puromycin resistance gene expression cassette

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

mouse PGK promoter

Available since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-ERBB3

Plasmid

#23874

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ERBB3 (ERBB3 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pLSC-5

Plasmid

#62889

Purpose

Lenti vector for expression of inducible split Cas9

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Inserts

SpCas9(574-1368)
SpCas9(2-573)

Use

CRISPR and Lentiviral

Tags

FFBP12, FRB, NES PTK2, NLS SV40, and P2A

Expression

Mammalian

Mutation

*see comment below

Promoter

EFS

Available since

March 26, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR-Flag-IKKalpha

Plasmid

#15467

Depositor

Hiroyasu Nakano

Article

Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

Insert

IkB kinase alpha (Chuk Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 10, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc-GFPC1-Sec61beta

Plasmid

#15108

Depositor

Tom Rapoport

Insert

Sec61beta (SEC61B Human)

Use

Tags

Ac GFP

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-AKT-PH-GFP

Plasmid

#18836

Depositor

Craig Montell

Article

Kwon et al Mol Cell. 2007 Feb 23. 25(4):491-503.

Insert

AKT-PH domain (AKT1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

contains amino acids 1-148 (compared to GenBank r…

Promoter

CMV

Available since

July 14, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pDS-RED-C1-Beclin1

Plasmid

#24405

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Use

Tags

DsRed

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pAMPK alpha2 WT

Plasmid

#15991

Depositor

Morris Birnbaum

Article

Mu et al Mol Cell. 2001 May . 7(5):1085-94.

Insert

AMPK alpha2 (Prkaa2 Rat)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
M57 pPET28a-TEV-full-length human beta catenin

Plasmid

#17198

Depositor

Randall Moon

Insert

beta catenin (CTNNB1 Human)

Use

Tags

HIS

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK1043

Plasmid

#8835

Depositor

David Waugh

Article

Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

Insert

tobacco etch virus protease, catalytic domain

Use

Tags

MBP and polyarginine

Expression

Bacterial

Mutation

Autolysis-resistant S219V mutant

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-FLAG LAP2

Plasmid

#15738

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

LAP2 (CEBPB Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
p3962 pcDNA3-IRF3-FL

Plasmid

#22860

Depositor

Peter Howley

Article

Kirshner et al J Virol. 2005 Jul . 79(14):9320-4.

Insert

IRF3 (IRF3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pDZ Flag USP28

Plasmid

#15665

Depositor

Martin Eilers

Article

Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

Insert

USP28 (USP28 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2007

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pMJ841

Plasmid

#39318

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A) and Histidine 840 …

Promoter

T7

Available since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe K-Ras 12V

Plasmid

#12544

Depositor

Channing Der

Article

Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

Insert

K-Ras 12V (KRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

12V constituitively activated

Promoter

Available since

Sept. 8, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pECE

Plasmid

#26453

Depositor

William Rutter

Article

Ellis et al Cell. 1986 Jun 6;45(5):721-32.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pTE4396

Plasmid

#74041

Purpose

Expresses human codon-optimized AsCpf1 and As crRNA.

Depositor

Ervin Welker

Article

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Inserts

As crRNA
AsCpf1

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Mutation

Promoter

CMV and human U6

Available since

April 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge