4,180 results

1. pWZL-Blast-Flag-HA-hTERT

   Plasmid

   #22396

   Depositor

   William Hahn

   Article

   Maida et al Nature. 2009 Sep 10. 461(7261):230-5.

   Insert

   TERT (TERT Human)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Available Since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV5 TBRI-His

   Plasmid

   #19161

   Depositor

   Joan Massague

   Insert

   transforming growth factor, beta receptor 1 (TGFBR1 Human)
   

   Tags

   His

   Expression

   Mammalian

   Available Since

   Oct. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CMV-NLS-R-GECO

   Plasmid

   #32462

   Purpose

   Mammalian expression of nucleus-targeting red fluorescent genetically encoded Ca2+ indicator for optical imaging

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   R-GECO1.0
   

   Tags

   NLS sequence DPKKKRKV

   Expression

   Mammalian

   Mutation

   Substitutions relative to the mApple-derived anal…

   Promoter

   CMV

   Available Since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLV hUbC-dCas9 VP64-T2A-GFP

   Plasmid

   #53192

   Purpose

   Co-expresses human optimized S. pyogenes dCas9-VP64 and GFP

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Insert

   humanized dead Cas9 VP64 T2A GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D10A and H840A

   Promoter

   hUbC

   Available Since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBad-mKalama1

   Plasmid

   #14892

   Purpose

   Bacterial expression of mKalama1 blue fluorescent protein

   Depositor

   Robert Campbell

   Article

   Ai et al Biochemistry. 2007 May 22;46(20):5904-10

   Insert

   mKalama1
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   The fluorescent protein was inserted between XhoI…

   Available Since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pEGFP hsp70 K71E

   Plasmid

   #15216

   Depositor

   Lois Greene

   Article

   Zeng et al J Cell Sci. 2004 Oct 1. 117(Pt 21):4991-

   Insert

   hsp70 K71E (HSPA1A Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   K71E, ATPase-deficient mutant.

   Available Since

   June 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLL7.0: tgRFPt-SSPB R73Q

   Plasmid

   #60416

   Purpose

   Mammalian Expression of tgRFPt-Micro

   Depositor

   Brian Kuhlman

   Article

   Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

   Insert

   tgRFPt-SSPB R73Q
   

   Use

   Cre/Lox and Lentiviral

   Tags

   TagRFPt

   Expression

   Mammalian

   Mutation

   R73Q, Y11K and A15E

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GFP-rGBD

   Plasmid

   #26732

   Depositor

   William Bement

   Article

   Benink et al J Cell Biol. 2005 Jan 31. 168(3):429-39.

   Insert

   rhotekin (rtkn2 Mouse)
   

   Use

   Reporter for rho

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   RhoA binding domain of rhotekin

   Available Since

   Feb. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. C5V

   Plasmid

   #26394

   Depositor

   Steven Vogel

   Article

   Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

   Insert

   Cerulean-5-Venus
   

   Use

   Fret standard

   Tags

   Cerulean and Venus

   Expression

   Mammalian

   Available Since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. IL6-GFP

    Plasmid

    #28088

    Depositor

    Jennifer Stow

    Article

    Manderson et al J Cell Biol. 2007 Jul 2. 178(1):57-69.

    Insert

    IL6 (Il6 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG-FlpeERT2

    Plasmid

    #14756

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Flpe-ERT2 fusion protein
    

    Use

    Flp/frt

    Tags

    ERT2

    Expression

    Mammalian

    Available Since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR223_Luciferase

    Plasmid

    #25894

    Purpose

    Gateway entry vector containing Luciferase.

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Insert

    Luciferase
    

    Use

    Gateway entry vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPGK-Cre-bpA

    Plasmid

    #11543

    Purpose

    Expresses Cre recombinase from mouse PGK promoter in mammalian cells.

    Depositor

    Klaus Rajewsky

    Insert

    Phosphoglycerate kinase 1 promoter
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    mouse PGK

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HA-GbL

    Plasmid

    #1865

    Depositor

    David Sabatini

    Article

    Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

    Insert

    GbL (MLST8 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mRuby2-C1

    Plasmid

    #54768

    Purpose

    Localization: C1 Cloning Vector, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Type

    Empty backbone

    Tags

    mRuby2

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pGEM-Oct4

    Plasmid

    #16352

    Depositor

    James Thomson

    Article

    Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

    Insert

    Homo sapiens POU domain, class 5, transcription factor 1 (POU5F1) (POU5F1 Human)
    

    Expression

    Bacterial

    Available Since

    Jan. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. VCP(DK0)-EGFP

    Plasmid

    #23974

    Depositor

    Nico Dantuma

    Article

    Tresse et al Autophagy. 2010 Feb 22. 6(2):.

    Insert

    Valosin containing protein (VCP Human)
    

    Tags

    6xHis, EGFP, and HA

    Expression

    Mammalian

    Mutation

    E305QE578Q

    Available Since

    March 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HA-FOXO3a TM

    Plasmid

    #1788

    Depositor

    Michael Greenberg

    Article

    Brunet et al Cell 1999 Mar 19;96(6):857-68.

    Insert

    FOXO3a (FOXO3 Human)
    

    Tags

    HA (YDVPDYASL)

    Expression

    Mammalian

    Mutation

    T32A, S253A, S315A (no longer detectably phosphor…

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GFP-LKB1

    Plasmid

    #21147

    Depositor

    Junying Yuan

    Article

    Karuman et al Mol Cell. 2001 Jun . 7(6):1307-19.

    Insert

    GFP-LKB1 (Stk11 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    May 11, 2009

    Availability

    Academic Institutions and Nonprofits only

20. MSCV h c-MYC IRES GFP

    Plasmid

    #18119

    Depositor

    John Cleveland

    Insert

    c-MYC (MYC Human)
    

    Use

    Retroviral

    Tags

    GFP

    Mutation

    The CUG which is the start site for Myc I is pres…

    Available Since

    June 9, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge