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pcDNA3.1-3XHA-p53 Δ1-42

Plasmid

#196269

Purpose

Mammalian expression of 3xHA tagged p53 Δ1-42 (ΔTAD1)

Depositor

Laura Attardi

Article

Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

Insert

p53 Δ1-42 (Trp53 Mouse)

Tags

3xHA

Expression

Mammalian

Mutation

deleted amino acids 1 to 42

Promoter

CMV

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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Sema6c-AP-His

Plasmid

#72041

Purpose

Expresses the extracellular region of the Sema6C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6c (Sema6c Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMIGR1-HA-mTBK1

Plasmid

#174741

Purpose

Gene overexpression

Depositor

Shao-Cong Sun

Insert

TBK1 (Tbk1 Mouse)

Use

Retroviral

Mutation

WT

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pFUSEss-CHIg-mG2b_M18

Plasmid

#105931

Purpose

Expression plasmid coding for heavy chain of mouse IgG2b antibody specific to antigen B of the ABO blood group system, clone M18.

Depositor

Joanna Bereta

Article

Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

Insert

IgG2b (Ighg2b Mouse)

Expression

Mammalian

Promoter

hEF1-HTLV prom

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-WASF2-N

Plasmid

#55161

Purpose

Localization: Cytoskeleton, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

WASF2

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
Sema6b(L)-AP-His

Plasmid

#72039

Purpose

Expresses the extracellular region of the Sema6B protein (entire extracellular domain; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6b (Sema6b Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2.sgNf1.1

Plasmid

#91895

Purpose

sgNf1.1 for Nf1 deletion

Depositor

Wen Xue

Article

Song et al Gastroenterology. 2017 Apr;152(5):1161-1

Insert

sgNf1.1

Use

Lentiviral and Mouse Targeting

Expression

Mammalian

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pET28_mouse-Caspase-11_enzymatic-domain

Plasmid

#183382

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-11 (AA100-373) (Casp4 Mouse)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pSB700-mouse-ACTC1-Puro

Plasmid

#128326

Purpose

SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)

Depositor

Alejandro Chavez

Insert

gRNA against mouse ACTC1 for activation (Actc1 Mouse)

Expression

Mammalian

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-BDFP1.6

Plasmid

#197199

Purpose

Expresses the protein of BDFP1.6 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

BDFP1.6

Use

AAV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-SPHKAP [L131/17R]

Plasmid

#177477

Purpose

Mammalian Expression Plasmid of anti-SPHKAP (Mouse). Derived from hybridoma L131/17.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-SPHKAP (Mus musculus) recombinant mouse monoclonal antibody (Sphkap Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGL4 mIL-17 0.6kb promoter

Plasmid

#20126

Depositor

Warren Strober

Article

Fuping et al 2008 Nov;9(11):1297-306. Epub 2008 Oct 1

Insert

mIL-17 (Il17a Mouse)

Use

Luciferase

Available Since

Jan. 16, 2009

Availability

Academic Institutions and Nonprofits only
Pak3

Plasmid

#99510

Purpose

to express Pak3 in mammalian system

Depositor

Dong-Yan Jin

Article

Chan et al Retrovirology. 2013 Apr 26;10:47. doi: 1

Insert

Pak3 (Pak3 Mouse)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE-Bi-SG-T

Plasmid

#26084

Depositor

Liqun Luo

Article

Li et al PLoS One. 2010 . 5(7):e11503.

Insert

Synaptophysin (Syp Mouse)

Tags

mut4EGFP

Expression

Mammalian

Available Since

Sept. 8, 2010

Availability

Academic Institutions and Nonprofits only

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mCherry-Dynactin-N-18

Plasmid

#55034

Purpose

Localization: Dynein Receptor, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Dynactin

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG mFoxg1-GFP wildtype

Plasmid

#73048

Purpose

C-terminal fusion of gfp Mouse wildtype Foxg1

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

mFoxg1 (Foxg1 Mouse)

Expression

Mammalian

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pMVS142_pACT1_mCherry_Zif268_EBD_MCS_KAN

Plasmid

#99049

Purpose

Transcription Factor chassis for activation domains. mCherry, Zif268 DNA binding domain, estrogen response domain

Depositor

Barak Cohen

Article

Staller et al Cell Syst. 2018 Mar 1. pii: S2405-4712(1

Inserts

ACT1 promoter (ACT1 Budding Yeast)
mCherry
Zif268 DNA binding Domain
Estrogen response domain (ESR1 Human)

Expression

Yeast

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2B Flag BimEL

Plasmid

#23090

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

Bim EL (Bcl2l11 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only
pCDF-His-mTET1CD∆cat

Plasmid

#81054

Purpose

bacterial expression of catalytic dead catalytic domain of murine TET1

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TET1 (Tet1 Mouse)

Tags

6HIS

Expression

Bacterial

Mutation

catalytic domain amino acid 1367-2057 with histid…

Promoter

T7

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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RNF130 shRNA-TRE

Plasmid

#225336

Purpose

shRNAmir backbone under tet-operator for RNA interference, with YFP

Depositor

Huda Zoghbi

Article

Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

Insert

RNF130 shRNA (Rnf130 Mouse)

Use

AAV and RNAi

Expression

Mammalian

Promoter

TRE (tetracycline-responsive element)

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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