159,352 results

1. pHCMV_DENV2_PrME

   Plasmid

   #207264

   Purpose

   Mammalian expression of Dengue virus 2 PrME protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   DENV2_PrME (POLY Dengue virus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEPIP_Peptide_Cloning_Vector

   Plasmid

   #172110

   Purpose

   Lentiviral plasmid for protein / peptide overexpression

   Depositor

   Prashant Mali

   Article

   Ford et al Cell Syst. 2021 Jul 21;12(7):716-732.e7.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Ef1-alpha

   Available since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMSCV-IRES-GFP II (pMIG II)

   Plasmid

   #52107

   Purpose

   a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Protoc. 2006;1(1):406-17.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHSP02

   Plasmid

   #117259

   Purpose

   Genome editing for Lactobacillus plantarum WCSF1

   Depositor

   Sheng Yang

   Article

   Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

   Insert

   Cas9, promotor P11-guide-RNA, homologous arms of Lp_0537
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLVX-EF1a-EGFP-RAB7A-IRES-Puromycin

   Plasmid

   #133027

   Purpose

   Expresses EGFP-tagged late endosome marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   RAB7A (RAB7A Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Human elongation factor 1 alpha

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pU6 SgRNA RBM20

   Plasmid

   #162735

   Purpose

   Mammalian expression

   Depositor

   Amit Choudhary

   Article

   Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

   Insert

   Spacer sequence for RBM20
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pALS3-Ma-sfGFP-TAG150

   Plasmid

   #212121

   Purpose

   Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-TAG150 and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

   Depositor

   Ryan Mehl

   Inserts

   sfGFP-150TAG
   M. alvus Pyl-tRNA(6)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   N150TAG

   Promoter

   araC and lpp

   Available since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pALS3-Ma-sfGFP-WT

   Plasmid

   #212119

   Purpose

   Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

   Depositor

   Ryan Mehl

   Inserts

   SUMO-sfGFP-WT
   M. alvus Pyl-tRNA(6)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   araC and lpp

   Available since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBK-Ma pylRS nitroY/haloY-F5

   Plasmid

   #212125

   Purpose

   Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control for Ma Pyl tRNA-synthetase selections.

   Depositor

   Ryan Mehl

   Insert

   M. alvus PylRS 3-NY/HaloY F5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   CTG125CTT, N166A V168S, A223C, W239R

   Promoter

   GlnS (constitutive)

   Available since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTKEI-Tre-C04

    Plasmid

    #79754

    Purpose

    Single fluorescent protein biosensor for trehalose

    Depositor

    David Savage

    Article

    Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

    Insert

    Tre-C04
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    pTrc

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAGGs-Foxp1

    Plasmid

    #45720

    Depositor

    Jeremy Dasen

    Article

    Dasen et al Cell. 2008 Jul 25;134(2):304-16. doi: 10

    Insert

    FoxP1 (Foxp1 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    CMV / b-actin

    Available since

    Aug. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

12. MAS1-Tango

    Plasmid

    #66425

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    MAS1 (MAS1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pLKO.1 hygro

    Plasmid

    #24150

    Purpose

    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses hygromycin for selection.

    Depositor

    Bob Weinberg

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA

    Available since

    March 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TFORF2601

    Plasmid

    #144133

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    POU6F2 (POU6F2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. V5-miniTurbo-NES_pCDNA3

    Plasmid

    #107170

    Purpose

    expresses V5-tagged miniTurbo in the mammalian cytosol

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pR I-Ab beta Derp1-117

    Plasmid

    #113639

    Purpose

    Insect cell expression vector for I-Ab beta chain fused to Der p 1 117-127 antigenic peptide

    Depositor

    James Moon

    Article

    Moon et al Methods Mol Biol. 2018;1799:165-181. doi

    Insert

    I-Ab beta Derp1-117 (H2-Ab1 Mouse)
    

    Use

    Tags

    6x His tag, fused to Der p 1 (117-127) peptide vi…

    Expression

    Insect

    Mutation

    transmembrane and cytoplasmic domains truncated

    Promoter

    Metallothionein

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR223-RPS6KA1

    Plasmid

    #23860

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    RPS6KA1 (RPS6KA1 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    1MM

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAP03-sp44/p21

    Plasmid

    #120232

    Purpose

    Carries bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites that can be amplified with homology sequence for refactoring.

    Depositor

    Bradley Moore

    Article

    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

    Insert

    bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    aac(3)IV (apramycin resistance gene)

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAB078d5

    Plasmid

    #79204

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1) luxAB; Ppro1 434cI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ-opt (OR1); Ppro1

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBacPAK p110a WT

    Plasmid

    #55722

    Purpose

    His3 GST tagged WT p110 alpha

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    p110 alpha (Pik3ca Mouse)
    

    Use

    Tags

    His3 GST

    Expression

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only