We narrowed to 24,275 results for: CHI

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pGEX-E6AP Hect

Plasmid

#165102

Purpose

bacterial expression of a GST fusion of Human E6AP Hect domain

Depositor

Arthur Haas

Article

Ronchi et al Methods Mol Biol. 2012;832:197-218. doi:

Insert

E6AP Hect (UBE3A Human)

Tags

GST from plasmid.

Expression

Bacterial

Mutation

E6AP Hect domain Amino acids 495-738 (5'QLNP…

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGIPz-PD-L1 4NQ

Plasmid

#121487

Purpose

Expression of human PD-L1 4NQ with PD-L1 shRNA

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Inserts

PD-L1 4NQ
hPD-L1 shRNA

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

N35Q, N192Q, N200Q and N219Q

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-MICU1-mEF

Plasmid

#31765

Depositor

Vamsi Mootha

Article

Perocchi et al Nature. 2010 Sep 16;467(7313):291-6. Epu

Insert

MICU1 (MICU1 Human)

Use

Pdonr gateway

Tags

None

Mutation

Mutated in both EF-hand domains: D231A, E242K, D4…

Available Since

May 18, 2012

Availability

Academic Institutions and Nonprofits only

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pET32a-hBTC

Plasmid

#199230

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

Insert

Betacellulin (BTC Human)

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Promoter

T7

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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USPL1cat-pETM30

Plasmid

#85768

Purpose

bacterial expression of USPL1 catalytic domain (aa 212-514) as His-GST-TEVsite fusion

Depositor

Frauke Melchior

Article

Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

Insert

USPL1, catalytic domain (aa 212-514) (USPL1 Human)

Tags

GST and His

Expression

Bacterial

Mutation

catalytic domain (aa 212-514)

Promoter

T7-lac

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pBiFC-HA-hCaspase 2 FL (C303S)-VC155

Plasmid

#49257

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

caspase-2 (CASP2 Human)

Tags

HA and split Venus C

Expression

Mammalian

Mutation

C303S

Promoter

CMV

Available Since

March 14, 2014

Availability

Academic Institutions and Nonprofits only
pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5B 376-963

Plasmid

#134612

Purpose

Mammalian expression of mouse KIF5B 376-963

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF5B (Kif5b Mouse)

Tags

Halo

Expression

Mammalian

Mutation

WT with deletion of the 375 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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pOTTC446 - pAAV EF1a hPREP(S554A)

Plasmid

#59968

Purpose

An AAV packaging vector that expresses catalytically-inactive hPREP under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

Insert

Prolyl oligopeptidase (PREP Human)

Use

AAV

Expression

Mammalian

Mutation

S554A

Promoter

EF1a

Available Since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only

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EGFP-hPCM2w/b delta6

Plasmid

#47588

Depositor

Emanuel Strehler

Article

Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

Insert

PMCA2w/b delta6 (ATP2B2 Human)

Tags

EGFP-HA-His

Expression

Mammalian

Mutation

6 C-terminal AA deleted (needed for PDZ binding)

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-PU.1-ETS

Plasmid

#124652

Purpose

Chimeric ETS domain

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (SPI1, ETS1 Human)

Expression

Mammalian

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-dLight1.2

Plasmid

#111069

Purpose

To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has Service

AAV1

Insert

dLight1.2

Use

AAV

Tags

Flag tag

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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gZiPro

Plasmid

#198441

Purpose

Activity/inhibition assays, 19F-NMR

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Tags

His6-TEV

Expression

Bacterial

Mutation

R95*A,

Promoter

T7 promoter

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pDESTpK7WG-RPS5Ap-mito

Plasmid

#163145

Purpose

Plant mitoTALEN vector assembly, Destination vector for plants to express ORFs from entry clone with mitochondrial presequence under the RPS5A promoter

Depositor

Shin-ichi Arimura

Article

Arimura et al Plant J. 2020 Oct 24. doi: 10.1111/tpj.1

Insert

RPS5A promoter and mitochondrial presequence in front of attR1 in pk7WG

Expression

Plant

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-3xFLAG::Fnip1-pgk-hph

Plasmid

#48758

Purpose

PiggyBac backbone, CAG promoter driving murine 3xFLAG-Fnip1 fusion, Hygromycin selection

Depositor

Austin Smith

Article

Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

Insert

3xFLAG::Fnip1 (Fnip1 Mouse)

Use

Piggybac

Tags

3xFLAG

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only
pDONR P2R-P3-V5-6xHis

Plasmid

#48347

Purpose

Contributes the coding sequence of a V5-6xHis epitope tags cassette as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

Depositor

Anna Planas, Tomas Santalucia

Article

Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

Insert

V5 and 6xHis epitope tags cassette

Use

Gateway entry clone

Mutation

Contains a stop codon at the end of the epitope t…

Promoter

none

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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USPL1cat-pEYFP_C1

Plasmid

#85766

Purpose

mammalian expression of USPL1 catalytic domain (aa 212-514) as YFP fusion

Depositor

Frauke Melchior

Article

Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

Insert

USPL1, catalytic domain (aa 212-514) (USPL1 Human)

Tags

EYFP

Expression

Mammalian

Mutation

catalytic domain (aa 212-514)

Promoter

CMV

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF3B tail 391-747

Plasmid

#134615

Purpose

Mammalian expression of mouse KIF3B 391-747

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF3B (Kif5b Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 390 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221-MICU1-sh1-rORF

Plasmid

#31764

Depositor

Vamsi Mootha

Article

Perocchi et al Nature. 2010 Sep 16;467(7313):291-6. Epu

Insert

MICU1 (MICU1 Human)

Use

Pdonr gateway

Tags

None

Mutation

RNAi-resistant ORF (rORF). 8 synonymous changes i…

Promoter

None

Available Since

May 4, 2012

Availability

Academic Institutions and Nonprofits only

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pBa.FRB-3myc-GS-KIF3A tail 419-702

Plasmid

#134614

Purpose

Mammalian expression of human KIF3A 419-702

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF3A (KIF3A Human)

Tags

FRB, 3myc

Expression

Mammalian

Mutation

WT with deletion of the 418 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pET-Duet1-6xHis-TEV-LC3BGlydelta5C

Plasmid

#190237

Purpose

Plasmid for the expression and purification of 6xHis-TEV-LC3BGlydelta5C. Internal reference: SMC893.

Depositor

Sascha Martens

Article

Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

MAP1LC3B (MAP1LC3B Human)

Tags

6xHis-TEV

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAS_4xEcoLeuT(CUA)_EF1_AnapRS

Plasmid

#174894

Purpose

AnapRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

AnapRS

Expression

Mammalian

Mutation

L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF3C tail 411-796

Plasmid

#134616

Purpose

Mammalian expression of mouse KIF3C 411-796

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF3C (Kif3c Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 410 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pTRE Tight ArchT-GFP

Plasmid

#79627

Purpose

Tetracycline-dependent expression of ArchT-EGFP in mammalian cells

Depositor

Yasunori Hayashi

Article

Sakaguchi et al PLoS One. 2015 Jun 15;10(6):e0130163. do

Insert

ArchT

Tags

EGFP

Expression

Mammalian

Mutation

ArchT is a more sensitive version of Arch (archae…

Promoter

Tet responsive Ptight promoter

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only
PGEX-6P1-hNF2-AR mutant

Plasmid

#107149

Purpose

bacterial expression

Depositor

Tina Izard

Article

Chinthalapudi et al Nat Commun. 2018 Apr 6;9(1):1338. doi: 1

Insert

neurofibromin 2 (NF2 Human)

Tags

GST

Expression

Bacterial

Mutation

A585W-R588K

Promoter

Ptac

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1 A66V

Plasmid

#166817

Purpose

Gateway pDONR vector encoding NLRP1 with the patient mutation A66V (Zhong et al., 2016) (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Mutation

A66V

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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NET37 pEGFP-N2 (607)

Plasmid

#61990

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

NET37 (KIAA1161 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pET32a-hAREG

Plasmid

#199229

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

Insert

Amphiregulin preproprotein (AREG Human)

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR E2 HSPter-RPS5Ap-mito

Plasmid

#163146

Purpose

Plant mitoTALEN vector assembly, Entry clone 2 with attL1 L4; Multisite Gateway entry vector to terminate the E1 ORF and having RPS5A promoter with mitochondrial presequence for the E3 ORF

Depositor

Shin-ichi Arimura

Article

Arimura et al Plant J. 2020 Oct 24. doi: 10.1111/tpj.1

Insert

A terminator of Arabidopsis heat shock protein, RPS5A promoter, and mitochondrial presequence

Use

Entry vector

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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NET50 pmRFP-N2 (489)

Plasmid

#61983

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

Insert

NET50 (DHRS7 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pBa.GFP-KIF17 tail 400-1038

Plasmid

#134617

Purpose

Mammalian expression of mouse KIF1A 400-1038

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF17 (Kif17 Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 399 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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