We narrowed to 5,560 results for: Chia

1. VEGF120TAG

   Plasmid

   #200946

   Purpose

   Encodes recombinant mouse VEGF120 when coupled with amber stop codon suppression by non-canonical amino acid incorporation

   Depositor

   Elizabeth Kirby

   Article

   Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103

   Inserts

   VEGF120TAG (Vegfa Mouse)
   hPGK-driven Puromycin resistance
   

   Use

   Lentiviral

   Tags

   6x HIS

   Expression

   Mutation

   amber stop codon 101 bp from ATG

   Promoter

   Ef1a and hPGK

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. VEGF164TAG

   Plasmid

   #200947

   Purpose

   Encodes recombinant mouse VEGF164 when coupled with amber stop codon suppression by non-canonical amino acid incorporation

   Depositor

   Elizabeth Kirby

   Article

   Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103

   Inserts

   VEGF164TAG (Vegfa Mouse)
   hPGK-driven Puromycin resistance
   

   Use

   Lentiviral

   Tags

   6x HIS

   Expression

   Mutation

   amber stop codon 101 bp from ATG

   Promoter

   Ef1a and hPGK

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS-SMSr-TEV-Twin-Strep

   Plasmid

   #202530

   Purpose

   Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase-relate protein (SMSr) in mammalian cell

   Depositor

   Chiaki Murakami

   Article

   Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

   Insert

   SAMD8 (SAMD8 Human)
   

   Use

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   deleted stop codon (TGA)

   Promoter

   CAG and chicken β-actin promoter

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLY315

   Plasmid

   #184160

   Purpose

   Reporter circuit for hijacking mRNA of zntA as CRISPR RNA

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

   Inserts

   dCas9
   tetR
   pspFΔHTH::λN22plus
   sfgfp
   rhaS
   

   Use

   Synthetic Biology

   Tags

   ASV tag and λN22plus

   Expression

   Bacterial

   Mutation

   Mutations D10A and H840A on WT cas9. Synonymous m…

   Promoter

   Pcon, PpspA-zntA-1034, PrhaB, and Ptet

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLY162

   Plasmid

   #184138

   Purpose

   Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 1)

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

   Inserts

   dCas9
   tetR
   pspFΔHTH::λN22plus
   sfgfp
   rhaS
   

   Use

   Synthetic Biology

   Tags

   ASV tag and λN22plus

   Expression

   Bacterial

   Mutation

   Mutations D10A and H840A on WT cas9. Synonymous m…

   Promoter

   Pcon, PpspA-R1, PrhaB, and Ptet

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLY163

   Plasmid

   #184141

   Purpose

   Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 2)

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

   Inserts

   dCas9
   tetR
   pspFΔHTH::λN22plus
   sfgfp
   rhaS
   

   Use

   Synthetic Biology

   Tags

   ASV tag and λN22plus

   Expression

   Bacterial

   Mutation

   Mutations D10A and H840A on WT cas9. Synonymous m…

   Promoter

   Pcon, PpspA-R2, PrhaB, and Ptet

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLY168

   Plasmid

   #184142

   Purpose

   Reporter circuit for hijacking mRNA of arsenic responsive gene cluster as CRISPR RNA (site 2)

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

   Inserts

   dCas9
   tetR
   pspFΔHTH::λN22plus
   sfgfp
   rhaS
   

   Use

   Synthetic Biology

   Tags

   ASV tag and λN22plus

   Expression

   Bacterial

   Mutation

   Mutations D10A and H840A on WT cas9. Synonymous m…

   Promoter

   Pcon, PpspA-Ar2, PrhaB, and Ptet

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLY300

   Plasmid

   #184158

   Purpose

   Reporter circuit for hijacking small RNA oxyS as CRISPR RNA

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

   Inserts

   dCas9
   tetR
   pspFΔHTH::λN22plus
   sfgfp
   rhaS
   

   Use

   Synthetic Biology

   Tags

   ASV tag and λN22plus

   Expression

   Bacterial

   Mutation

   Mutations D10A and H840A on WT cas9. Synonymous m…

   Promoter

   Pcon, PpspA-oxyS-86, PrhaB, and Ptet

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLY320

   Plasmid

   #184161

   Purpose

   Reporter circuit for hijacking mRNA of cusCFAB as CRISPR RNA

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

   Inserts

   dCas9
   tetR
   pspFΔHTH::λN22plus
   sfgfp
   rhaS
   

   Use

   Synthetic Biology

   Tags

   ASV tag and λN22plus

   Expression

   Bacterial

   Mutation

   Mutations D10A and H840A on WT cas9. Synonymous m…

   Promoter

   Pcon, PpspA-cusCFAB-656, PrhaB, and Ptet

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSL008_phiC31_neo-5xTetO-pEF-H2B-mCitrine-5kb,lambda-pRSV-H2B-mCherry

    Plasmid

    #179426

    Purpose

    Dual fluorescent reporter construct with 5kb lambda spacer, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    TRE-cit-5kb-mch
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMH010_phiC31_neo-5xTetO-pEF-H2B-mCitrine-1.2kb,lambda-pRSV-H2B-mCherry

    Plasmid

    #179427

    Purpose

    Dual fluorescent reporter construct with 1.2kb lambda spacer, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    TRE-cit-1.2kb-mch
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5-Flag-BRD4-WT

    Plasmid

    #90331

    Purpose

    expresses Wild type BRD4

    Depositor

    Kornelia Polyak

    Article

    Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Wild type

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLENTI_hACE2_HygR

    Plasmid

    #155296

    Purpose

    Lentiviral vector to generate hACE2 stable expressing cell line, Hygromycin resistance

    Depositor

    Raffaele De Francesco

    Article

    Notarbartolo et al Sci Immunol. 2021 Aug 10;6(62). pii: 6/6

    Insert

    hACE2 (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    C9

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pDCAF3-IS5

    Plasmid

    #65220

    Purpose

    Expresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.

    Depositor

    Jeffrey Barrick

    Article

    Quandt et al ACS Synth Biol. 2013 Jun 21;2(6):301-7.

    Inserts

    ndmA
    ndmB
    ndmC
    ndmD
    gst9
    chlR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Changed start codon from gtg to atg and Changed s…

    Promoter

    BBa_J23100

    Available since

    Jan. 8, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pCDNA5-Flag-BRD4-BD

    Plasmid

    #90005

    Purpose

    expresses BD mutant BRD4

    Depositor

    Kornelia Polyak

    Article

    Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Changed 140N to 140A, 433N to 433A

    Promoter

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR221-TAF15 Stop

    Plasmid

    #84895

    Purpose

    DONOR vector for Gateway cloning of TAF15

    Depositor

    Aaron Gitler

    Article

    Couthouis et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

    Insert

    TAF15 (TAF15 Human)
    

    Use

    Gateway donor

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTO_CHMP4B_LAP

    Plasmid

    #101850

    Purpose

    Tet-inducible expression of human CHMP4B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration

    Depositor

    Daniel Gerlich

    Article

    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d

    Insert

    Charged Multivesicular Body Protein 4B (CHMP4B Human)
    

    Use

    Gateway cloning, flp-frt recombination, tet-induc…

    Tags

    Localization and Affinity Purification (LAP) tag

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcrDNA3.1-Chimera

    Plasmid

    #100891

    Purpose

    Mammalian expression of DNAJB1(exon1)-PRKACA(exon2-Cterminus) chimeric protein from fibrolamellar hepatocellular carcinoma

    Depositor

    Sanford Simon

    Article

    Honeyman et al Science. 2014 Feb 28;343(6174):1010-4. d

    Insert

    Major Fibrolamellar hepatocellular carcinoma Chimera (DNAJB1, PRKACA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AiP1011-pAAV-mscRE4-minBGpromoter-tTA2-WPRE-hGHpA

    Plasmid

    #163480

    Purpose

    Direct-expressing tTA2 AAV Virus. Alias: AiP1011 - pAAV-AiE2004m-minBG-tTA2-WPRE-HGHpA

    Depositor

    The Allen Institute for Brain Science, Bosiljka Tasic

    Article

    Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

    Insert

    tTA2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Beta Globin minimal promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. AiP1002-pAAV-mscRE16-minBGpromoter-EGFP-WPRE-hGHpA

    Plasmid

    #163486

    Purpose

    Direct-expressing EGFP AAV Virus. Alias: AiP1002 - pAAV-AiE2016m-minBG-EGFP-WPRE-HGHpA

    Depositor

    The Allen Institute for Brain Science, Bosiljka Tasic

    Article

    Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Beta Globin minimal promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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