We narrowed to 18,889 results for: Tec

1. p1.2-Zeo

   Plasmid

   #162740

   Purpose

   Protein expression in CHO cells

   Depositor

   Ivan Vorobiev

   Article

   Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   CHO Elongation factor 1A

   Available Since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS416 Gal-RNQ1-YFP

   Plasmid

   #18685

   Depositor

   Susan Lindquist

   Article

   Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

   Insert

   Rnq1 (RNQ1 Budding Yeast)
   

   Tags

   YFP

   Expression

   Yeast

   Available Since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

3. tdEos-Paxillin-22

   Plasmid

   #57653

   Purpose

   Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

   Insert

   Paxillin
   

   Tags

   tdEos

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 13, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pJ291_WT_med

   Plasmid

   #128856

   Purpose

   Medium copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

   Depositor

   Mark Pallen

   Article

   Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

   Insert

   Flagellin
   

   Expression

   Bacterial

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCfB2374

   Plasmid

   #67534

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked KlURA3 marker, integration into S. cerevisiae XI-1 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Expression

   Yeast

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. 6xHis-MBP-huHkCas12a

   Plasmid

   #174683

   Purpose

   Expresses 6xHis-MBP tagged HkCas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huHkCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDM_noro_S6_lacZA

   Plasmid

   #118817

   Purpose

   T7 RNAP-driven expression of norovirus sense orientation toehold switches (S6) with a lacZ alpha subunit reporter.

   Depositor

   Alexander Green

   Article

   Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

   Insert

   Norovirus toehold switch S6
   

   Expression

   Bacterial

   Available Since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVH234-Tier1-PmPGK1-tTA

   Plasmid

   #169574

   Purpose

   Tier-1 vector encoding PmPGK1-driven tTA expression (PmPGK1-tTA-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   tetracycline-controlled transactivator
   

   Expression

   Mammalian

   Promoter

   PPGK

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSET FLIPE-10u

   Plasmid

   #13538

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E A207R
   

   Tags

   ECFP and Venus

   Expression

   Bacterial

   Mutation

   A207R in binding protein

   Available Since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. GGE020

    Plasmid

    #120777

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for junction of position1-3. When using the GG platform for expressing only one gene (T1-3_Lip2)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    T1-3_Lip2
    

    Use

    Cloning/assembly plasmid

    Available Since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCellFree_G03 NFX1

    Plasmid

    #67131

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    NFX1 (NFX1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pBT100.110

    Plasmid

    #122602

    Purpose

    clone CDA1 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    CDA1
    

    Expression

    Bacterial

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCfB4796

    Plasmid

    #106131

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntD_1, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMS22H

    Plasmid

    #133904

    Purpose

    Expression of MS2BS-RNAsite hybrid. Homology regions for recombination with pAWH

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTK101

    Plasmid

    #59561

    Purpose

    SIN SP6 P1234 (nsP2 P726S) SGP(14) mKate-G8-L7Ae-PEST 4xFF4

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP(14) mKate-G8-L7Ae-PEST 4xFF4
    

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBW829_pCAG-pExpr-LUT

    Plasmid

    #87554

    Purpose

    Encodes Boolean Look-up Table logic in response to Cre, Flp, PhiC31, Vika, B3, and Bxb1 recombinases

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    EGFP
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. YSBE 559 : pNAT_6xHA

    Plasmid

    #92087

    Purpose

    Plasmid for tagging proteins in Cryptococcus neoformans; 6xHA tag, NAT selection

    Depositor

    Yong-Sun Bahn

    Article

    So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

    Insert

    6xHA
    

    Use

    tagging proteins in cryptococcus neoforman

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFLIP38

    Plasmid

    #65616

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Tags

    6His-edCit-attR1 and attR2-edCer-cMyc

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. Id2-Halo donor

    Plasmid

    #113116

    Purpose

    Donor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Id2 coding sequence

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Id2 donor region including homology arms and HaloTag
    

    Use

    Synthetic Biology

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. YIp211-P30-His-POL30-3'

    Plasmid

    #99546

    Purpose

    Yeast integrative vector for expression of His-tagged POL30 (PCNA); URA3 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    POL30 (POL30 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Yeast

    Promoter

    POL30

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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