19,635 results

1. pP{ELAV-GeneSwitch}

   Plasmid

   #83957

   Purpose

   Expresses conditional RU486-dependent GAL4 protein under control of the elav promotor

   Depositor

   Haig Keshishian

   Article

   Osterwalder et al Proc Natl Acad Sci U S A. 2001 Oct 23;98

   Insert

   fusion protein consisting of the GAL4-DBD, human progesterone receptor-ligand-binding domain, and P65-AD (GAL4, RELA Budding Yeast, Human)
   

   Expression

   Insect

   Promoter

   elav

   Available Since

   Dec. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETcon(-)

   Plasmid

   #41522

   Depositor

   Andrew Scharenberg

   Type

   Empty backbone

   Tags

   3x(G4S) Linker, G3S Linker, HA, Myc Tag, and Stuf…

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. Influenza-FPV-HA-mEGFP

   Plasmid

   #127810

   Purpose

   Expression vector Influenza A virus FPV (H7N1) hemagglutinin mEGFP fusion protein, plasma membrane localization

   Depositor

   Salvatore Chiantia

   Article

   Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

   Insert

   Influenza A virus FPV (H7N1) hemagglutinin mEGFP fusion
   

   Expression

   Mammalian

   Available Since

   June 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-PBZ-mRuby2

   Plasmid

   #110650

   Purpose

   expresses a PAR-binding (PBZ) domain fused to mRuby2

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

   Insert

   PBZ domain from APLF, amino acids 371-451 (APLF Human)
   

   Tags

   mRuby2

   Expression

   Mammalian

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. spNW25

   Plasmid

   #173484

   Purpose

   Expresses spNW25 in Bl21

   Depositor

   Huan Bao

   Article

   Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

   Insert

   spNW25
   

   Tags

   Histag

   Expression

   Bacterial

   Available Since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV-Mito4x-jRCaMP1b

   Plasmid

   #127873

   Purpose

   Red-shifted fluorescent reporter for mitochondrial matrix Ca2+

   Depositor

   Timothy Ryan

   Article

   Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

   Insert

   Red-shifted mitochondrial matrix Ca2+ indicator
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST-CMV-3xFLAG-gateway-EGFP

   Plasmid

   #122845

   Purpose

   N-terminal 3xFLAG tag and C-terminal EGFP tag. Gateway destination vector for mammalian expression.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   3xFLAG and EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTHSSe_50

   Plasmid

   #109248

   Purpose

   incW BbsI cloning vector

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   sfGFP
   

   Expression

   Bacterial

   Promoter

   PJ23105

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEMS-II-PEST

   Plasmid

   #204766

   Purpose

   GEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)

   Depositor

   Kate Meyer

   Article

   Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   EGFP-PEST-m6Alinker-DHFR
   

   Mutation

   mutated all RACs in EGFP to RGC/RCC

   Promoter

   CMV

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin

    Plasmid

    #134868

    Purpose

    Expresses EGFP-tagged lysosomal marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Lysosomal-associated membrane protein 1 (LAMP1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. FLAG-tag

    Plasmid

    #55180

    Purpose

    FLAG-tag

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    FLAG
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Available Since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. FU-tet-o-hc-myc

    Plasmid

    #19775

    Depositor

    Konrad Hochedlinger

    Article

    Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

    Insert

    c-myc (MYC Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    T58A

    Available Since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG1159 TOM20-ECFP-FIREmate

    Plasmid

    #196693

    Purpose

    Expresses TOM20-ECFP-FIREmate in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    TOM20-ECFP-FIREmate
    

    Tags

    FIREmate

    Expression

    Mammalian

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-TO-hNIL BSD-mApple

    Plasmid

    #182313

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression, bsd selection, mApple

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Inserts

    hNGN2 (NEUROG2 Human)
    hISL1 (ISL1 Human)
    hLHX3 (LHX3 Human)
    bsd-T2A-mycNLS-mApple
    

    Tags

    T2A-mycNLS-mApple

    Expression

    Mammalian

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-TetO-hNGN2-Puro

    Plasmid

    #79049

    Purpose

    Expresses human NEUROGENIN2 (hNGN2) and puromycin resistance gene under control of TetON promoter. This lentiviral vector is used to generate NGN2-iNs (induced neurons) from hiPSCs and hiPSC-NPCs.

    Depositor

    Kristen Brennand

    Article

    Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15

    Insert

    hNGN2-P2A-PuroR (NEUROG2 Human)
    

    Use

    Lentiviral

    Promoter

    TetO

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. mito-PAGFP

    Plasmid

    #23348

    Purpose

    Expresses a mitochondrial matrix-targeted photoactivable GFP that enables detection and quantification of organelle fusion in living cells.

    Depositor

    Richard Youle

    Article

    Karbowski et al J Cell Biol. 2004 Feb 16. 164(4):493-9.

    Insert

    PAGFP
    

    Tags

    mito

    Expression

    Mammalian

    Available Since

    April 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-HP1beta

    Plasmid

    #17651

    Depositor

    Tom Misteli

    Article

    Cheutin et al Science. 2003 Jan 31. 299(5607):721-5.

    Insert

    HP1 beta (CBX1 Human)
    

    Tags

    GFP and His

    Expression

    Mammalian

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-HP1gamma

    Plasmid

    #17650

    Depositor

    Tom Misteli

    Article

    Cheutin et al Science. 2003 Jan 31. 299(5607):721-5.

    Insert

    HP1 gamma (CBX3 Human)
    

    Tags

    GFP and His

    Expression

    Mammalian

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3-HA-Akt1

    Plasmid

    #73408

    Purpose

    Expresses human AKT1 with a N-terminus HA tag

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    AKT1 (AKT1 Human)
    

    Tags

    HA tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. myc-hULK1

    Plasmid

    #31961

    Depositor

    Do-Hyung Kim

    Article

    Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

    Insert

    ULK1 (ULK1 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV, SP6

    Available Since

    Sept. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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