We narrowed to 9,302 results for: BLI;
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Plasmid#233936PurposeMammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-17xTA with alanine mutation in the TRAF6 binding motifDepositorInsertCHARMS-17xTA-3xA
UseLentiviralTagsmEGFPExpressionMammalianPromoterSV40Available SinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHRSV_CHARMS-20xTA-3xAla
Plasmid#233937PurposeMammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-20xTA with alanine mutation in the TRAF6 binding motifDepositorInsertCHARMS-20xTA-20xA
UseLentiviralTagsmEGFPExpressionMammalianPromoterSV40Available SinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHRSV_CHARMS-TIR-5xT6BMs
Plasmid#233938PurposeMammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-TIR with 5 TRAF6 binding motifsDepositorInsertCHARMS-TIR-5xT6BMs
UseLentiviralTagsmEGFPExpressionMammalianPromoterSV40Available SinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
insulator_seq_UENU_donor
Plasmid#221459PurposeVector from which reporter gene 3'UTRs, an enhancer, and a neutral DNA spacer are cloned for assembling an insulator-seq reporter library.DepositorInsert3'UTR-OpIE2 enhancer-neutral DNA spacer-3'UTR
UseSynthetic BiologyAvailable SinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
insulator_seq_reporter_vector
Plasmid#221460PurposeVector containing eGFP and mCherry reporter genes, for assembling an insulator-seq reporter library.DepositorInsertgypsy insulator-EGFP-neutral spacer DNA-mCherry
UseSynthetic BiologyAvailable SinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-eGFP-Pep-H
Plasmid#185788PurposeEncodes enhanced green flourescent protein linked to Pep-H self-assembling peptide via a GS-linker sequenceDepositorInserteGFP-Pep4
TagsHis-tagExpressionBacterialPromoterT7Available SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
GGE207
Plasmid#120792PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assemblyDepositorInsertInsDown-Mfe
UseCloning/assembly plasmidAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
B108-Cre2
Plasmid#73990PurposeB108 enhancer of Blimp1 gene drives IRES-Cre expression.DepositorInsertB108-IRES-Cre
TagsMycExpressionMammalianAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pLX_HA
Plasmid#178534PurposeModifies pLX_317 backbone to express c-term HA tag (instead of c-term V5 tag)DepositorTypeEmpty backboneTagsHAExpressionMammalianPromoterEF1aAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
dClover2-C1
Plasmid#54577PurposeLocalization: C1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.DepositorTypeEmpty backboneTagsdClover2ExpressionMammalianAvailable SinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
HER2Nb-C AND (A OR T)-SpyTag
Plasmid#243790PurposeExpresses multifunctional construct enabling the AND(OR)-gated release of a HER2 Nanobody from materials in response to HRV-3C AND (sortase eSrtA(2A9) OR TEV) treatment.DepositorInsertHER2 nanobody
Tags6x His tag and SsrAExpressionBacterialAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pE-FLP
Plasmid#45978PurposeExpresses yeast Flp driven by the constitutive promoter pE from phage P2DepositorInsertflp
UseSynthetic BiologyExpressionBacterialMutationflp driven by the constitutive promoter pE from p…PromoterpE (from phage P2)Available SinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pB-GLUT4-7myc-GFP
Plasmid#52872Purposedetection and measurement of GLUT4 translocationDepositorInsertGLUT4 (Slc2a4 Rat, with human c-terminus)
UseRetroviralTags7x-myc (internal) and GFPExpressionMammalianAvailable SinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMA2640
Plasmid#25434PurposeRetroviral Tet-On vector for CMV-driven rtTA with EGFP and Blasticidin selectionDepositorInsertreverse tetracycline-regulated transactivator Advanced
UseRetroviralExpressionMammalianPromoterCMVAvailable SinceJune 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
C1-FT-mCitrine-EEVEE-CaaX
Plasmid#155225PurposeMPAct with increased linker flexiblityDepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with a codon optimized EEVEE and CaaX seq derived from Kras4b
TagsmCitrineExpressionMammalianAvailable SinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBG-hUBP43
Plasmid#12455DepositorInsertUBP43 (USP18 Human)
TagsGSTExpressionMammalianMutationpEBG vector is from Bruce Mayer, may be different…Available SinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Avitag-SpyCatcher002
Plasmid#102828PurposeBacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylationDepositorInsertAvitag-SpyCatcher002
TagsAvitag, GSSGS, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
p426MET25_SSKar2-sfpHluorin-HDEL (ER-sfpHluorin, MRV66)
Plasmid#115698PurposeER-targeted superfolder pHluorin for improved pH measurements in the ER of S. cerevisiaeDepositorInsertSSKar2-sfpHluorin-HDEL
TagsER retention signal containing HDEL and ER signal…ExpressionYeastMutationfor introduced mutations see publicationPromoterMET25Available SinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR R4 ATCC LacZ GCTT
Plasmid#173668PurposeDestination vector for Golden Gate Cloning assemblies, blue-white screeningDepositorTypeEmpty backboneExpressionBacterial and InsectAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRE:CloverCP-EFS:ZeoR
Plasmid#68478PurposeLentivirus for establishment of doxycycline-inducible cell line expression CloverCPDepositorInsertsCloverCP
Zeocin Resistance
UseLentiviralTags"CP" degradation domainExpressionMammalianPromoterEFS and pTREAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pPEE17
Plasmid#128361PurposeDetecting gene essentiality in Cryptococcus neoformans. Targets DNA constructs to the Safe Haven Site 1, a small gene-free region. Contains amdS2 counterselectable marker. This version of the Safe Haven vector includes direct repeats, enabling the formation of popouts on fluoroacetamide.DepositorInsertAcetamidase (amdS)
UseUnspecifiedPromoterTEF1Available SinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)WF
Plasmid#108337PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)BR
Plasmid#108336PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)WF
Plasmid#108333PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)BF
Plasmid#108334PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)WR
Plasmid#108335PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)BF
Plasmid#108338PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)WR
Plasmid#108339PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)BR
Plasmid#108340PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-T-SpyTag
Plasmid#243787PurposeExpresses multifunctional construct enabling the YES-gated release of mCerulean from materials in response to TEV treatment. Construct contains a SpyTag for material tethering.DepositorInsertmCerulean
Tags6x His tagExpressionBacterialAvailable SinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern-A OR (S AND T)-SpyTag
Plasmid#243781PurposeExpresses multifunctional construct enabling the OR(AND)-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) OR (sortase eSrtA(4S9) AND TEV) treatment. Contains a SpyTag for material tethering.DepositorInsertmGreenLantern
Tags6x His tagExpressionBacterialAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern-S OR (A AND T)-SpyTag
Plasmid#243782PurposeExpresses multifunctional construct enabling the OR(AND)-gated release of mGreenLantern from materials in response to sortase eSrtA(4S9) OR (sortase eSrtA(2A9) AND TEV) treatment. Contains a SpyTag for material tethering.DepositorInsertmGreenLantern
Tags6x His tagExpressionBacterialAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern-T OR (A AND S)-SpyTag
Plasmid#243783PurposeExpresses multifunctional construct enabling the OR(AND)-gated release of mGreenLantern from materials in response to TEV OR (sortase eSrtA(2A9) AND sortase eSrtA(4S9) ) treatment. Contains a SpyTag for material tethering.DepositorInsertmGreenLantern
Tags6x His tagExpressionBacterialAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern-A OR S OR T-SpyTag
Plasmid#243784PurposeExpresses multifunctional construct enabling the OR OR-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) OR sortase eSrtA(4S9) OR TEV treatment. Contains a SpyTag for material tethering.DepositorInsertmGreenLantern
Tags6x His tagExpressionBacterialAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern-A AND S AND T-SpyTag
Plasmid#243785PurposeExpresses multifunctional construct enabling the AND AND-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) AND sortase eSrtA(4S9) AND TEV treatment. Contains a SpyTag for material tethering.DepositorInsertmGreenLantern
Tags6x His tagExpressionBacterialAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mCherry-S-SpyTag
Plasmid#243786PurposeExpresses multifunctional construct enabling the YES-gated release of mCherry from materials in response to sortase eSrtA(4S9) treatment. Construct contains a SpyTag for material tethering.DepositorInsertmCherry
Tags6x His tagExpressionBacterialAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-S AND T-SpyTag
Plasmid#243788PurposeExpresses multifunctional construct enabling the AND-gated release of mCerulean from materials in response to sortase eSrtA(4S9) AND TEV treatment. Contains a SpyTag for material tethering.DepositorInsertmCerulean
Tags6x His tag and SsrAExpressionBacterialAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern-C OR (T AND V)-CAAX
Plasmid#243791PurposeExpresses multifunctional construct enabling the OR(AND)-gated release of mGreenLantern from membranes in response to HRV-3C OR (TEV AND TVMV) treatment. Construct contains a CAAX tag for intracellular membrane tethering.DepositorInsertmGreenLantern
UseLentiviralTagsCAAX TagAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern-A-SpyTag
Plasmid#243769PurposeExpresses multifunctional construct enabling the YES-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) treatment. Construct contains a SpyTag for material tethering.DepositorInsertmGreenLantern
Tags6x His tagExpressionBacterialAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern-S-SpyTag
Plasmid#243770PurposeExpresses multifunctional construct enabling the YES-gated release of mGreenLantern from materials in response to sortase eSrtA(4S9) treatment. Construct contains a SpyTag for material tethering.DepositorInsertmGreenLantern
Tags6x His tagExpressionBacterialAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only