We narrowed to 9,004 results for: Pol

1. KG901: pMVP (L3-L2) HA epitope tag + polyA

   Plasmid

   #121749

   Purpose

   pMVP L3-L2 entry plasmid, contains HA epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   HA epitope tag + polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. YIp128-P30-His-pol30(K127R)

   Plasmid

   #99543

   Purpose

   Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127R; Leu2 marker

   Depositor

   Helle Ulrich

   Article

   Davies et al Methods Mol Biol. 2012;920:543-67. doi:

   Insert

   POL30 (POL30 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   K127R

   Promoter

   POL30

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. attB-GFP-Dnmt3b6-Poly(A)-NeoR

   Plasmid

   #65552

   Purpose

   Plasmid for Bxb1-mediated recombination of the GFP-Dnmt3b6 cDNA into a MIN-tagged locus using Neomycin selection

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   Dnmt3b (Dnmt3b Mouse)
   

   Use

   Mouse Targeting; Bxb1

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   isoform 6

   Promoter

   Available since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-3xsgRNA_Opn1mw-RHO-Cas9N-IntN-polyA

   Plasmid

   #165450

   Purpose

   Express N-terminal part of split dCas9-VPR and 3 sgRNAs targeting murine Opn1mw promoter

   Depositor

   Elvir Becirovic

   Article

   Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

   Inserts

   dCas9N-IntN
   3x Opn1mw promoter-targeting sgRNAs
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and short human rhodopsin promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-RHO-IntC-dCas9C-VPR-polyA

   Plasmid

   #166692

   Purpose

   Express C-terminal part of split dCas9-VPR

   Depositor

   Stylianos Michalakis

   Article

   Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

   Insert

   IntC-dCas9C-VPR
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   short human rhodopsin promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA 3.1(Hygro) myc-hPolQ-Flag

   Plasmid

   #73132

   Purpose

   To express in mammalian cells human PolQ

   Depositor

   Agnel Sfeir

   Article

   Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

   Insert

   Polymerase theta (POLQ Human)
   

   Use

   Tags

   Flag and myc

   Expression

   Mammalian

   Mutation

   Promoter

   cmv promoter

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19-TgKI-MCS-eGFP_Y40N-MCS-polyA

   Plasmid

   #174025

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   eGFP
   

   Use

   Zebrafish knock-in tagging

   Tags

   Expression

   Mutation

   Y40N

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJRH-1346 U6-B2M sgRNA Gag-pol v2

   Plasmid

   #201917

   Purpose

   Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Inserts

   Gag-pol
   B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and Human U6

   Available since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA

   Plasmid

   #192552

   Purpose

   Expresses nuclear localized tdTomato under CAG promoter

   Depositor

   Guoping Feng

   Has Service

   AAV PHP.eB and AAV9

   Insert

   tdTomato
   

   Use

   AAV

   Tags

   NLS

   Expression

   Mutation

   deleted E273 and G274 compared to FPbase ID: PGG5S

   Promoter

   CAG

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCIneo-hCHD7-Kozak ATG 3' HA-bGH polyA

    Plasmid

    #89460

    Purpose

    constitutive expression of human CHD7 in mammalian cells

    Depositor

    Peter Scacheri

    Insert

    CHD7 (CHD7 Human)
    

    Use

    Tags

    hinge + HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGP-CAG-iGABASnFR2n-WPRE-bGH-polyA

    Plasmid

    #218869

    Purpose

    Mammalian expression of improved GABA sensor (negative change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2n
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S99A F104H R168P

    Promoter

    CAG

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. single polypeptide FPX biosensor for calcium ion

    Plasmid

    #60887

    Purpose

    FPX biosensor

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    single polypeptide FPX biosensor for calcium ion
    

    Use

    Tags

    HindIII site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-bpNLS-hPol eta-2A-tagBFP-bGHpA

    Plasmid

    #193968

    Purpose

    Encodes a wildtype human Pol? (eta) with P2A-tagBFP marker driven by CAG promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    hPol eta (POLH Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZH23 Dual G-less containing ySSA1 polyA

    Plasmid

    #231647

    Purpose

    yeast S.cerevisiae SSA1 polyA termination region inserted between the dual G-less cassette

    Depositor

    Jesper Svejstrup

    Article

    Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

    Insert

    SSA1 polyA termination region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZH20 Dual G-less containing yCYC1 polyA

    Plasmid

    #231646

    Purpose

    yeast S.cerevisiae CYC1 polyA termination region inserted between the dual G-less cassette

    Depositor

    Jesper Svejstrup

    Article

    Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

    Insert

    CYC1 polyA termination region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUC19-TgKI-MCS-eGFP-MCS-polyA

    Plasmid

    #174024

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    eGFP
    

    Use

    Zebrafish knock-in tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CAG-EGFP barcode-1-SV40 polyA

    Plasmid

    #190865

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-CAG-EGFP barcode-0-SV40 polyA

    Plasmid

    #190864

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-CAG-EGFP barcode-3-SV40 polyA

    Plasmid

    #190867

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode3
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-CAG-EGFP barcode-4-SV40 polyA

    Plasmid

    #190868

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode4
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-CAG-EGFP barcode-2-SV40 polyA

    Plasmid

    #190866

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGP-CAG-iGABASnFR2-WPRE-bGH-polyA

    Plasmid

    #218868

    Purpose

    Mammalian expression of improved GABA sensor (positive change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S99A F102Y F104Y L178S

    Promoter

    CAG

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. KL157 - pBlueScript II SK-Grb10_HA-T2A-eGFP-SV40PolyA

    Plasmid

    #232935

    Purpose

    Donor plasmid for knock-in of T2A-eGFP-SV40pA at the mouse Grb10 locus. Used with PX459-sgRNA048_Grb10 sgRNA.

    Depositor

    Danny Reinberg

    Article

    Lucero et al bioRxiv, March 19, 2025

    Insert

    T2A-eGFP-SV40pA with Grb10 homology arms
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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24. 8xGliBS-IVS2-mCherry-NLS-polyA-Tol2

    Plasmid

    #84604

    Purpose

    Tol2-based hedgehog signal reporter plasmid for zebrafish transgenesis

    Depositor

    James Chen

    Article

    Mich et al PLoS One. 2014 Jul 28;9(7):e103661. doi:

    Insert

    mCherry
    

    Use

    Zebrafish transgenesis

    Tags

    Expression

    Mutation

    Promoter

    8xGliBS-delta-crystallin minimal promoter

    Available since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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25. PB-CAG-CasRX-P2A-EGFP-BGH PolyA

    Plasmid

    #154004

    Purpose

    Express CasRx

    Depositor

    Hui Yang

    Article

    He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

    Insert

    CasRx
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. PB-3XpolyA-3XPC-miniCMV-mCherry-WPRE-insulator

    Plasmid

    #168291

    Purpose

    optimized miniCMV (OminiCMV)

    Depositor

    Hui Yang

    Article

    Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    miniCMV

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pUC19-TgKI-MCS-p2A-tdTomato-polyA

    Plasmid

    #174027

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    p2A-tdTomato
    

    Use

    Zebrafish knock-in tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-CAG-EGFP barcode-5-SV40 polyA

    Plasmid

    #190869

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode5
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLV-CMV-EGFP-PolB-PAMmut-Hygro

    Plasmid

    #176086

    Purpose

    EGFP fused to the N-terminus of a POLB containing a mutation in the PAM site used by POLBKOg1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Mutation in the PAM site used by PolBKOg1

    Promoter

    CMV

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. Polymerase expression - pCMV-Phi29(+exo) (LM2759)

    Plasmid

    #208964

    Purpose

    Unfused Phi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Phi29(+exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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