Addgene

We narrowed to 6,367 results for: poly

Showing: 511 - 540 of 6367 results

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pCI-MS2V5-PABPC1 MLLEmut M161A/D165K

Plasmid

#65810

Purpose

expression of PABPC1-MS2 fusion, double mutant.

Depositor

Niels Gehring

Article

Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

Insert

PABPC1 (PABPC4 Human)

Use

Tags

MS2 stem loops and V5

Expression

Mammalian

Mutation

MLLEmut M161A/D165K

Promoter

CMV

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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C035

Plasmid

#174341

Purpose

Template for making Rluc mRNA reporter with pA120.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

Renilla luciferase

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pME.E (pDHg16)

Plasmid

#24573

Depositor

David Pulleyblank

Article

Haniford et al Nature. 1983 Apr 14. 302(5909):632-4.

Insert

pME.E (pDHg16)

Use

Tags

Expression

Mutation

Promoter

Available since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
C098

Plasmid

#174345

Purpose

Template for making Nluc mRNA reporter with pA139.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

NanoLuc luciferase

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2024

Availability

Academic Institutions and Nonprofits only

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C034

Plasmid

#174340

Purpose

Template for making Rluc mRNA reporter with pA98.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

Renilla luciferase

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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C033

Plasmid

#174339

Purpose

Template for making Rluc mRNA reporter with pA63.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

Renilla luciferase

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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C031

Plasmid

#174338

Purpose

Template for making Rluc mRNA reporter with pA30.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

Renilla luciferase

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
C054

Plasmid

#174342

Purpose

Template for making Fluc mRNA reporter with pA124 and mutant mouse Malat1.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

Firefly luciferase

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

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C071

Plasmid

#174343

Purpose

Template for making Nluc mRNA reporter with pA29.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

NanoLuc luciferase

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
C093

Plasmid

#174344

Purpose

Template for making Nluc mRNA reporter with pA63.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

NanoLuc luciferase

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pME.C (pDHf14)

Plasmid

#24572

Depositor

David Pulleyblank

Article

Haniford et al Nature. 1983 Apr 14. 302(5909):632-4.

Insert

Use

Tags

Expression

Mutation

Promoter

Available since

May 3, 2010

Availability

Academic Institutions and Nonprofits only
C079

Plasmid

#174348

Purpose

Template for making mRNA for overexpressing eGFP in frog oocytes

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

eGFP

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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C022

Plasmid

#174337

Purpose

Template for making Rluc mRNA reporter with no pA.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

Renilla luciferase

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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C278

Plasmid

#174356

Purpose

For introducing multiple copies of OsTIR1 with the piggyBac system

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

OsTIR1

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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C194

Plasmid

#174357

Purpose

For expressing GFP in human cell lines via transfection

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pENTR4 PARP2 E614Q

Plasmid

#192545

Purpose

pENTR plasmid Arabidopsis thaliana PARP2 CDS, E614Q mutation, no stop codon

Depositor

Lennart Wirthmueller

Article

Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

Insert

poly(ADP-ribose) polymerase 2 E614Q (AT3G18250 Mustard Weed)

Use

Gateway entry vector

Tags

Expression

Mutation

changed Glu 614 to Gln

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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C255

Plasmid

#174352

Purpose

Template for making mRNA for overexpressing eGFP (C-FLAG+HA) in frog oocytes

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

eGFP

Use

In vitro transcription template

Tags

FLAG and HA

Expression

Mutation

Promoter

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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C152

Plasmid

#174355

Purpose

Repair plasmid for introducing AID, T2A and mCherry at the C-teminus of human PABPC1

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

Auxin-inducible degron (AID) and mCherry

Use

Tags

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pENTR4 Ta-sro1 WWE-PARP

Plasmid

#192548

Purpose

pENTR plasmid Triticum aestivum sro1, amino acids 1-434, cultivar SR3, no stop codon

Depositor

Lennart Wirthmueller

Article

Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

Insert

SIMILAR TO RCD ONE 1 (SRO1) WWE-PARP domains, cultivar Shanrong No. 3 (SR3)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-TFE3pA

Plasmid

#120201

Purpose

Human TFE3 poly(A) site reporter

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

TFE3 poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pPS1307

Plasmid

#8912

Depositor

Pamela Silver

Article

Henry et al Mol Cell Biol. 1996 Jul . 16(7):3668-78.

Insert

HMT1 (HMT1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-RPS5pA

Plasmid

#120194

Purpose

Human RPS5 poly(A) site reporter

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

RPS5 poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-ILVBLpA

Plasmid

#120205

Purpose

Human ILVBL poly(A) site reporter

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

ILVBL poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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C150

Plasmid

#174350

Purpose

Template for making mRNA for overexpressing human eIF4G (C-FLAG) in frog oocytes

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

eIF4G1

Use

In vitro transcription template

Tags

FLAG

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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C245

Plasmid

#174351

Purpose

Template for making mRNA for overexpressing frog RPL3 (C-HA) in frog oocytes

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

rpl3.L (rpl3.L Frog)

Use

In vitro transcription template

Tags

HA

Expression

Mutation

Promoter

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-CENPBpA

Plasmid

#120162

Purpose

EGFP followed by the poly(A) site from human CENPB gene

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pRET.IL.PGK-TK

Plasmid

#1837

Depositor

Philip Leder

Article

Ishida et al Nucleic Acids Res 1999 Dec 15;27(24):e35

Type

Empty backbone

Use

Cre/Lox and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
pRET.IL.IRES-EGFP (No HSV-TK)

Plasmid

#1836

Depositor

Philip Leder

Article

Ishida et al Nucleic Acids Res 1999 Dec 15;27(24):e35

Type

Empty backbone

Use

Cre/Lox and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
pRET.IS.IRES-EGFP N2

Plasmid

#1834

Depositor

Philip Leder

Article

Ishida et al Nucleic Acids Res 1999 Dec 15;27(24):e35

Type

Empty backbone

Use

Cre/Lox and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
pRET.IIS.IRES-EGFP

Plasmid

#1838

Depositor

Philip Leder

Article

Ishida et al Nucleic Acids Res 1999 Dec 15;27(24):e35

Type

Empty backbone

Use

Cre/Lox and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2006

Availability

Academic Institutions and Nonprofits only