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Plasmid#67422PurposeExpression of hArl6 with C-term V5 and His6 tag in E. coliDepositorInsertARL6 (ARL6 Human)
UseTagsHis and V5ExpressionBacterialMutationPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL8B-no stop
Plasmid#67335PurposehArl8b without stop codon in Gateway Entry vectorDepositorInsertARL8B (ARL8B Human)
UseGateway entry vectorTagsExpressionBacterialMutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL6-no stop
Plasmid#67329PurposehArl6 without stop codon in Gateway Entry vectorDepositorInsertARL6 (ARL6 Human)
UseGateway entry vectorTagsExpressionBacterialMutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL11-no stop
Plasmid#67341PurposehArl11 without stop codon in Gateway Entry vectorDepositorInsertARL11 (ARL11 Human)
UseGateway entry vectorTagsExpressionBacterialMutationbp 442 T to C; aa C148RPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-ARL6-V5-His6
Plasmid#67443PurposeMammalian expression of hArl6 with C-term V5 and His6 tagDepositorInsertARL6 (ARL6 Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL1-no stop
Plasmid#67317PurposehArl1 without stop codon in Gateway Entry vectorDepositorInsertARL1 (ARL1 Human)
UseGateway entry vectorTagsExpressionBacterialMutationbp 231 C to T; silent mutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eBE-S1
Plasmid#101092PurposeExpresses eBE-S1 in mammalian cellsDepositorInserteBE-S1
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARL4C-V5-His6
Plasmid#67423PurposeExpression of hArl4c with C-term V5 and His6 tag in E. coliDepositorInsertARL7 (ARL4C Human)
UseTagsHis and V5ExpressionBacterialMutationPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL11
Plasmid#67340PurposehArl11 with stop codon in Gateway Entry vectorDepositorInsertARL11 (ARL11 Human)
UseGateway entry vectorTagsExpressionBacterialMutationbp 261 G to A; silent mutation; bp 442 T to C; aa…PromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWzyE-His
Plasmid#196669PurposeExpression of C-terminally His10-tagged WzyE from Pectobacterium atrosepticum in E.coliDepositorInsertWzyE
UseTagsTEV cleavable His10ExpressionBacterialMutationPromoteraraBADAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_T804N_hPGK1_mScarlet-I-TOSI_Donor
Plasmid#173208PurposeHDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the mScarlet-I mTOR signaling indicator (TOSI) cassette to ATP1A1 intron 17.DepositorInsertATP1A1-T804N mScarlet-I-TOSI donor
UseCRISPRTagsExpressionMammalianMutationPromoterhPGK1Available sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-MAFG-V5-miniTurbo
Plasmid#207818PurposeC-terminal tagged MAFG for miniTurbo proximity experimentsDepositorInsertMAFG-V5-miniTurbo
UseLentiviralTagsV5-miniTurboExpressionMutationPromoterUBCAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-MAFG
Plasmid#207817PurposeN-terminal tagged MAFG for miniTurbo proximity experimentsDepositorInsertminiTurbo-V5-MAFG
UseLentiviralTagsminiTurbo-V5ExpressionMutationPromoterUBCAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cas9-NS-A
Plasmid#207823PurposeDual expression of Cas9 and non-specific sgRNADepositorInsertnon-specific sgRNA
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterEF-1a; U6Available sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cas9-NS-B
Plasmid#207824PurposeDual expression of Cas9 and non-specific sgRNADepositorInsertnon-specific sgRNA
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterEF-1a; U6Available sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-ZNF746-C571A
Plasmid#207826PurposeDox-inducible expression of ZNF746-C571ADepositorInsertZNF746-C571A (ZNF746 Human)
UseLentiviralTagsExpressionMutationC571APromoterTREAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-zeo G226A E321K ORF29
Plasmid#200026PurposeLentiviral vector for expression of KSHV ORF29 with G226A and E321K mutationDepositorInsertORF29
UseLentiviralTagsExpressionMutationGlycine 226 changed to alanine, glutamic acid 321…PromoterCMVAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pminiT-mEmerald-MED1 donor
Plasmid#197867PurposeDonor plasmid used for building mEmerald-MED1 knock-in mESCsDepositorInsertCRSP210 (Med1 Mouse)
UseMouse TargetingTagsmEmeraldExpressionMutationPromoterNAAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTij_sg_mmu_Med6_C_terminal
Plasmid#197869PurposeCRISPR/Cas9/sgRNA plasmid for cutting Med6 C terminal and building MED6-mEmerald/Halo knock-in mESCsDepositorInsertMed6 (Med6 Mouse)
UseTagsExpressionMammalianMutationPromoterU6Available sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G3_EBFP_Nick_Dual_sgRNA
Plasmid#178094PurposeControl vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and EBFP Nick sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G3 + EBFP nick sgRNAs
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G3_EGFP_Nick_Dual_sgRNA
Plasmid#178095PurposeControl vector for coselection for PE3b in human cells. Tandem expression of ATP1A1 G3 and EGFP Nick sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G3 + EGFP nick sgRNAs
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G3_MTOR_Nick_Exon-44_Dual_sgRNA
Plasmid#178096PurposeControl vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick exon-44 sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G3 + MTOR nick exon-44 sgRNAs
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G3_MTOR_Nick_Intron-45_Dual_sgRNA
Plasmid#178099PurposeControl vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick intron-45 sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G3 + MTOR nick intron-45 sgRNAs
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G4_Q118R_MTOR_F2108L_Dual_pegRNA
Plasmid#178102PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-F2108L pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G4 Q118R pegRNA + MTOR-F2108L pegRNA
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G4_Q118R_MTOR_I2017T_Dual_pegRNA
Plasmid#178103PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-I2017T pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G4 Q118R pegRNA + MTOR-I2017T pegRNA
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G8_MTOR_Nick_Exon-44_Dual_sgRNA
Plasmid#178105PurposeControl vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick exon-44 sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G8 + MTOR nick exon-44 sgRNAs
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G8_MTOR_Nick_Intron-45_Dual_sgRNA
Plasmid#178106PurposeControl vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick intron-45 sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G8 + MTOR nick intron-45 sgRNAs
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G6_T804N_MTOR_I2017T_Dual_pegRNA
Plasmid#178108PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-I2017T pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G6 T804N pegRNA + MTOR-I2017T pegRNA
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G6_T804N_MTOR_F2108L_Dual_pegRNA
Plasmid#178109PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-F2108L pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G6 T804N pegRNA + MTOR-F2108L pegRNA
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G4_Q118R_MTOR_E2419K_Dual_pegRNA
Plasmid#173209PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-E2419K pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G4 Q118R pegRNA + MTOR E2419K pegRNA
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQLink-GST-2xCTD
Plasmid#138470PurposeExpresses GST-tagged 2xCTD heptapeptide repeat in bacteriaDepositorInsertRpb1 (POLR2A Human)
UseTagsGSTExpressionBacterialMutation2x CTD repeatPromoterT5Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF24 R68A-3xFLAG
Plasmid#138431PurposeExpresses full-length ORF24 R68A with a C-terminal 3xFLAG tag in mammalian cellsDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsFLAGExpressionMammalianMutationAmino acid R68 mutated to alaninePromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF24 R72A-3xFLAG
Plasmid#138432PurposeExpresses full-length ORF24 R72A with a C-terminal 3xFLAG tag in mammalian cellsDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsFLAGExpressionMammalianMutationAmino acid R72 mutated to alaninePromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF24 L73A-3xFLAG
Plasmid#138426PurposeExpresses full-length ORF24 L73A with a C-terminal 3xFLAG tag in mammalian cellsDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsFLAGExpressionMammalianMutationAmino acid L73 mutated to alaninePromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF24 L74A-3xFLAG
Plasmid#138427PurposeExpresses full-length ORF24 L74A with a C-terminal 3xFLAG tag in mammalian cellsDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsFLAGExpressionMammalianMutationAmino acid L74 mutated to alaninePromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF24 L75A-3xFLAG
Plasmid#138428PurposeExpresses full-length ORF24 L75A with a C-terminal 3xFLAG tag in mammalian cellsDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsFLAGExpressionMammalianMutationAmino acid L75 mutated to alaninePromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARL4
Plasmid#67461PurposeMammalian expression of hArl4 with stop codon (native expression)DepositorInsertARL4 (ARL4A Human)
UseTagsExpressionMammalianMutationbp 105 A to T; silent mutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARL5A
Plasmid#67462PurposeMammalian expression of hArl5 with stop codon (native expression)DepositorInsertARL5A (ARL5A Human)
UseTagsExpressionMammalianMutationbp 70 C to T; silent mutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARL14
Plasmid#67469PurposeMammalian expression of hArl14 with stop codon (native expression)DepositorInsertARL14 (ARL14 Human)
UseTagsExpressionMammalianMutationbp 172 C to T; aa L58FPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-ARL4-V5-His6
Plasmid#67440PurposeMammalian expression of hArl4 with C-term V5 and His6 tagDepositorInsertARL4 (ARL4A Human)
UseTagsHis and V5ExpressionMammalianMutationbp 105 A to T; silent mutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only