We narrowed to 2,493 results for: ERK

1. pQP-iRIS

   Plasmid

   #102584

   Purpose

   Regulation of protein localization (near-infrared-blue-light-inducible shuttle)

   Depositor

   Vladislav Verkhusha

   Article

   Redchuk et al Nat Chem Biol. 2017 Mar 27. doi: 10.1038

   Insert

   BphP1-mVenus-CAAX-IRES2-NES-mCherry-QPAS1-AsLOV2cNLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. piGECInano-N1

   Plasmid

   #186190

   Purpose

   Expression of small FRET-based near-infrared genetically-encoded calcium indicator in mammalian cells

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Front Cell Dev Biol. 2022 Jun 29;10:8801

   Insert

   iGECInano
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTagRFP675-N1

   Plasmid

   #44275

   Depositor

   Vladislav Verkhusha

   Article

   Piatkevich et al Sci Rep. 2013;3:1847. doi: 10.1038/srep0

   Insert

   TagRFP675
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   M41Q/F80W/S143N/L147M/S158N/D159Y/N173S

   Promoter

   CMV

   Available since

   May 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBAD/His-miRFP703

   Plasmid

   #79985

   Purpose

   Monomeric near-infrared fluorescent protein miRFP703 in bacterial plasmid

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

   Insert

   miRFP703
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araB

   Available since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBAD/HisB-PAmKate

   Plasmid

   #32691

   Depositor

   Vladislav Verkhusha

   Article

   Gunewardene et al Biophys J. 2011 Sep 21;101(6):1522-8. Ep

   Insert

   PAmKate
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   F84W/S148N/S165G compared to mKate (but numbering…

   Promoter

   araB

   Available since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNIR-Fbactin

   Plasmid

   #184677

   Purpose

   NIR-Fb to actin

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   NIR-Fbactin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSG5-CRKL-Y198FY207F

   Plasmid

   #32506

   Depositor

   Nora Heisterkamp, John Groffen

   Article

   de Jong et al Oncogene. 1997 Feb 6;14(5):507-13.

   Insert

   CRKL (CRKL Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Y207F and Y198F

   Promoter

   Available since

   Jan. 26, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pGEX-2T Crkl

   Plasmid

   #36400

   Depositor

   Nora Heisterkamp

   Article

   ten Hoeve et al Cancer Res. 1994 May 15;54(10):2563-7.

   Insert

   Crkl (CRKL Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 21, 2012

   Availability

   Academic Institutions and Nonprofits only

9. miRFP718nano-based inflammation reporter

   Plasmid

   #195753

   Purpose

   Reporter of inflamation

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

   Insert

   NF-kB-RE-miRFP718nano
   

   Use

   Tags

   miRFP718nano

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBAD/HisD-moxMaple3

    Plasmid

    #120871

    Purpose

    Bacterial expression of moxMaple3

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.

    Insert

    moxMaple3
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    from mMaple3, moxMaple3 was engineered by replaci…

    Promoter

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA6 MCV sTco

    Plasmid

    #40201

    Depositor

    Patrick Moore

    Article

    Shuda et al J Clin Invest. 2011 Sep;121(9):3623-34.

    Insert

    MCPyV sT codon optimized
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLSSmOrange-alpha-Tubulin

    Plasmid

    #37137

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

    Insert

    LSSmOrange
    

    Use

    Tags

    human alpha-tubulin

    Expression

    Mammalian

    Mutation

    A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

    Promoter

    CMV

    Available since

    June 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. peZinCh-2

    Plasmid

    #70008

    Purpose

    expresses eZinCh-2 Zn2+ FRET sensor in the cytosol of mammalian cells

    Depositor

    Maarten Merkx

    Article

    Hessels et al ACS Chem Biol. 2015 Sep 18;10(9):2126-34

    Insert

    eZinCh-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBABE-puro-NLS-LSSmKate2

    Plasmid

    #34586

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    NLS-LSS-mKate2
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

    Promoter

    Available since

    Feb. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIRESNEO-Scambio-HA

    Plasmid

    #33354

    Depositor

    Nora Heisterkamp, John Groffen

    Article

    Curtis et al Mol Cancer. 2004 Apr 23;3:10.

    Insert

    Scambio (ARHGEF40 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pCMVMagFRET-1-NLS

    Plasmid

    #50748

    Purpose

    Expresses MagFRET-1 in the nucleus of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal Ca2+ levels.

    Depositor

    Maarten Merkx

    Article

    Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

    Insert

    gene encoding for MagFRET-1 with NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPAiRFP1-N1

    Plasmid

    #44272

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Nat Commun. 2013 Jul 10;4:2153. doi: 10.

    Insert

    PAiRFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G127D/S141R/M163L/Q168L/A203V/G218S/R220P/V244F/A…

    Promoter

    CMV

    Available since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMito-iRFP720

    Plasmid

    #45464

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP720
    

    Use

    Tags

    human cytochrome C oxidase subunit VIII

    Expression

    Mammalian

    Mutation

    T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

    Promoter

    CMV

    Available since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNIR_PKAbiosensor

    Plasmid

    #127439

    Purpose

    Mammalian expression of the NIR biosensor for detecting PKA activity

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    NIR PKA biosensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNIR_JNKbiosensor

    Plasmid

    #127440

    Purpose

    Mammalian expression of the NIR biosensor for detecting JNK activity

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    NIR JNK biosensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBAD/His-miRFP709

    Plasmid

    #79986

    Purpose

    Monomeric near-infrared fluorescent protein miRFP709 in bacterial plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    miRFP709
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araB

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLSSmOrange-beta-Actin

    Plasmid

    #37136

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

    Insert

    LSSmOrange
    

    Use

    Tags

    human beta-actin

    Expression

    Mammalian

    Mutation

    A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

    Promoter

    CMV

    Available since

    June 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-G12-AkaLuc

    Plasmid

    #219551

    Purpose

    AkaLuc expression in mammlian cells. pAAV vector. Reporter plasmid.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

    Insert

    AkaLuc
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    12xUAS with TATA-box minimal promoter

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pG12-Rluc8

    Plasmid

    #170272

    Purpose

    Renilla reniformisluciferase (RLuc8) expression in mammlian cells. Reporter plasmid.

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

    Insert

    RLuc8
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    12xUAS with TATA-box minimal promoter

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pC4-RHE-PAS (FRB-PAS)

    Plasmid

    #80002

    Purpose

    miRFP PAS domain fused to FRB

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    FRB
    

    Use

    Tags

    PAS domain of miRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. peCALWY-NB

    Plasmid

    #22239

    Depositor

    Maarten Merkx

    Article

    Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

    Insert

    eCALWY non-binding variant
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C284S, C287S, C413S and C416S mutations linker c…

    Promoter

    Available since

    Jan. 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEB1-2xEGFP

    Plasmid

    #37827

    Depositor

    Torsten Wittmann

    Article

    Gierke et al Curr Biol. 2012 May 8;22(9):753-62. Epub

    Insert

    EB1 (MAPRE1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pC4EN-F1-mGAF709 (NLS-FKBP-mGAF709)

    Plasmid

    #80004

    Purpose

    miRFP709 GAF domain fused to FKBP and NLS

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    FKBP
    

    Use

    Tags

    GAF domain of miRFP709 and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pC4EN-F1-mGAF670 (FKBP-mGAF670)

    Plasmid

    #80003

    Purpose

    miRFP670 GAF domain fused to FKBP

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    FKBP
    

    Use

    Tags

    GAF domain of miRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBAD/HisD-TagRFP675

    Plasmid

    #44274

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Sci Rep. 2013;3:1847. doi: 10.1038/srep0

    Insert

    TagRFP675
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    M41Q/F80W/S143N/L147M/S158N/D159Y/N173S

    Promoter

    pBAD

    Available since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pWA PAS-E BAD K-GAFm

    Plasmid

    #39866

    Depositor

    Vladislav Verkhusha

    Article

    Filonov et al Chem Biol. 2013 Jul 23. pii: S1074-5521(

    Inserts

    iRFP protein PAS domain
    iRFP protein GAF domain (mutated)
    

    Use

    Tags

    E coil and K coil

    Expression

    Bacterial

    Mutation

    F165Y, D232Y, W308R

    Promoter

    arabinose inducible and rhamnose inducible

    Available since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMito-iRFP713

    Plasmid

    #45465

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP713
    

    Use

    Tags

    human cytochrome C oxidase subunit VIII

    Expression

    Mammalian

    Mutation

    T2A, S13L, A92T, V104T, V114I, E161K, Y193K, F198…

    Promoter

    CMV

    Available since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pVimentin-LSSmOrange

    Plasmid

    #37134

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

    Insert

    LSSmOrange
    

    Use

    Tags

    human cytochrome C oxidase subunit VIII and human…

    Expression

    Mammalian

    Mutation

    A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

    Promoter

    CMV

    Available since

    June 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pT2/SVNeo-103

    Plasmid

    #79841

    Purpose

    Expresses RpBphP1-mCherry-TetR under CMVd1 promoter

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

    Insert

    RpBphP1-mCherry-Intersectin1 DHPH domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMVd1

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Spa-1 in pCCL-c-MNDU3c-(X2)-pgk-EGFP

    Plasmid

    #38154

    Depositor

    Nora Heisterkamp

    Article

    Yi et al Int J Mol Med. 2008 Oct . 22(4):453-8.

    Inserts

    Spa-1 (SIPA1 Human)
    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Aug. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

36. pNIR-Fb2E7

    Plasmid

    #184681

    Purpose

    NIR-Fb to HIV gp41

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    NIR-Fb2E7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBABE-puro-NLS-LSSmKate1

    Plasmid

    #31873

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    NLS-LSS-mKate1
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

    Promoter

    Available since

    Dec. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Drp1(A395D)-StrepII

    Plasmid

    #174430

    Purpose

    Expresses human Drp1 Isoform 3 with A395D mutation in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1
    

    Use

    Tags

    StrepII

    Expression

    Bacterial

    Mutation

    A395D

    Promoter

    T7

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Drp1-StrepII

    Plasmid

    #174428

    Purpose

    Expresses human Drp1 Isoform 3 in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1
    

    Use

    Tags

    StrepII

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBAD-iGECInano

    Plasmid

    #186189

    Purpose

    Expression of small FRET-based near-infrared genetically-encoded calcium indicator in bacteria

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Front Cell Dev Biol. 2022 Jun 29;10:8801

    Insert

    iGECInano
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    AraC

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge