Addgene

We narrowed to 1,468 results for: pBluescript

Showing: 681 - 720 of 1468 results

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pMK388 (mClover-BSD)

Plasmid

#121189

Purpose

C-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

mClover-BSD

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pFloxin-IRES-Ofd1-Myc

Plasmid

#24559

Depositor

Jeremy Reiter

Article

Singla et al Nat Methods. 2010 Jan . 7(1):50-2.

Insert

Ofd1 (Ofd1 Mouse)

Use

Cre/Lox and Mouse Targeting

Tags

Myc

Expression

Mutation

Promoter

Available since

May 27, 2010

Availability

Academic Institutions and Nonprofits only

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pCAG-Trex2-bpA

Plasmid

#86984

Purpose

Expression of mouse Trex2

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Insert

mouse Trex2 coding region (Trex2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pBS-mitfa

Plasmid

#81230

Purpose

Cloning of zebrafish mitfa gene

Depositor

David Raible, James Lister

Article

Lister et al Development. 1999 Sep;126(17):3757-67.

Insert

mitfa (mitfa Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T3

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fCLIP-Kan

Plasmid

#188923

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fCLIP-Kan

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pg50-2.11 (#65)

Plasmid

#35966

Depositor

Thomas Sato

Article

Schlaeger et al Proc Natl Acad Sci U S A. 1997 Apr 1;94(

Insert

Tie2 promoter-1st exon-1st intron

Use

Tags

Expression

Mutation

Promoter

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
pBS-mPlexinD1

Plasmid

#16424

Depositor

Jonathan Epstein

Article

Gitler et al Dev Cell. 2004 Jul . 7(1):107-16.

Insert

Plexin D1 (Plxnd1 Mouse)

Use

In situ template

Tags

Expression

Mutation

None

Promoter

Available since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only
pBS NFATC1

Plasmid

#19203

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

NFATC1 (NFATC1 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
Mouse NHERF-1

Plasmid

#32950

Depositor

Edward Weinman

Article

Weinman et al Biochim Biophys Acta. 1999 Oct 6;1447(1)

Insert

Slc9a3R1 (Nherf1 Mouse)

Use

Tags

none

Expression

Bacterial

Mutation

none

Promoter

t3

Available since

Jan. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pmTUBB3

Plasmid

#31236

Depositor

Cliff Ragsdale

Article

Grove et al Development. 1998 Jun . 125(12):2315-25.

Insert

TUBB3 (Tubb3 Mouse)

Use

Cdna for in situ hybridization

Tags

Expression

Mutation

3'UTR sequence

Promoter

Available since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only
pAtLCYbSK

Plasmid

#53588

Purpose

Produces an active Arabidopsis thaliana lycopene beta-cyclase enzyme in E. coli. Also known as pAt9SK.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Eukaryot Cell. 2007 Mar;6(3):533-45. Epu

Insert

lcyB (LYC Mustard Weed)

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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PL06008A2C06 (smedwi2)

Plasmid

#35698

Depositor

Phillip Newmark

Article

Zayas et al Proc Natl Acad Sci U S A. 2005 Dec 20;10

Insert

Smedwi2

Use

Tags

Expression

Mutation

Promoter

T7 sense, T3 antisense

Available since

April 2, 2012

Availability

Academic Institutions and Nonprofits only
pBS Tenomodulin

Plasmid

#19200

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Tenomodulin (TNMD Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pBS Dynein light chain 1

Plasmid

#19213

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Dynein light chain 1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pHBFD1

Plasmid

#53565

Purpose

Produces an active HBFD (carotenoid 4-hydroxy-β-ring 4-dehydrogenase) enzyme in E. coli.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

Insert

hbfd1 cDNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-Histone H3

Plasmid

#45086

Depositor

Phillip Newmark

Article

Rouhana et al Development. 2014 Jul;141(13):2592-601.

Insert

Histone H3

Use

RNAi; In vitro transcription

Tags

Expression

Mutation

Promoter

T7 sense, T3 antisense

Available since

May 30, 2013

Availability

Academic Institutions and Nonprofits only
PRRX2-pBLSK

Plasmid

#21012

Depositor

Corey Largman

Article

Stelnicki et al J Invest Dermatol 1998 Jul;111(1):57-63.

Insert

PRRX2 (Prrx2 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Missing first three amino acids at N-terminus

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pPK720

Plasmid

#38147

Depositor

Patricia Kuwabara

Article

Soloviev et al Dev Biol. 2011 Mar 15;351(2):242-53. Epu

Insert

dpy-7p::ptc-3::gfp (ptc-3 )

Use

Tags

EGFP

Expression

Worm

Mutation

Promoter

dpy-7

Available since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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mCherry pBSDONR P4r-P2

Plasmid

#121528

Purpose

For making C-terminal mCherry fusions using multisite Gateway cloning

Depositor

Roger Innes

Article

Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

Insert

mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pPK348

Plasmid

#38145

Depositor

Patricia Kuwabara

Article

Soloviev et al Dev Biol. 2011 Mar 15;351(2):242-53. Epu

Insert

ptc-3::GFP (ptc-3 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

ptc-3

Available since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pBS-NT-AscI

Plasmid

#15461

Depositor

Peter Mombaerts

Article

Walz et al J Neurobiol. 2006 Jul . 66(8):835-46.

Insert

neurotensin (Nts Mouse)

Use

Tags

Expression

Mutation

Added AscI site 3 bp 3' of stop codon

Promoter

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pDHJS3 AN-

Plasmid

#78241

Purpose

One of four plasmids needed to create the mismatched double Holliday junction substrate (MM-DHJS).

Depositor

Tao-shih Hsieh

Article

Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

Inserts

loxP
sequence "A" with NheI site
loxP2

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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14-3-3 KO vector

Plasmid

#16551

Depositor

Bert Vogelstein

Article

Chan et al Nature. 1999 Oct 7. 401(6753):616-20.

Insert

14-3-3 knockout (SFN Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
pDHJS3 AS-

Plasmid

#78237

Purpose

One of four plasmids needed to create the double Holliday junction substrate (DHJS).

Depositor

Tao-shih Hsieh

Article

Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

Inserts

loxP
sequence "B"
loxP2

Use

Phagemid

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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3CB1/cSprouty2

Plasmid

#22096

Depositor

Gail Martin

Article

Minowada et al Development. 1999 Oct . 126(20):4465-75.

Insert

Sprouty 2 (SPRY2 Chicken)

Use

Bacterial

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
pFloxin-Ofd1-Myc

Plasmid

#24558

Depositor

Jeremy Reiter

Article

Singla et al Nat Methods. 2010 Jan . 7(1):50-2.

Insert

Ofd1 (Ofd1 Mouse)

Use

Cre/Lox and Mouse Targeting

Tags

Myc

Expression

Mutation

includes exons 4-23 only

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
cpi-17

Plasmid

#78708

Purpose

Encodes zebrafish cpi-17

Depositor

Sarah Childs

Article

Georgijevic et al Dev Dyn. 2007 Jun;236(6):1623-32.

Insert

cpi-17 (ppp1r14aa Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only
CpcB•PHLS+Cpc

Plasmid

#73335

Purpose

Expresses the PHLS gene as a fusion with the CpcB gene followed by the CmR gene

Depositor

Anastasios Melis

Article

Formighieri et al Metab Eng. 2015 Nov;32:116-24. doi: 10.1

Insert

cpcB•PHLS-CmR

Use

Tags

Expression

Mutation

Promoter

cpc operon

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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PL06010B1H03 (smedwi1)

Plasmid

#35699

Depositor

Phillip Newmark

Article

Zayas et al Proc Natl Acad Sci U S A. 2005 Dec 20;10

Insert

Smedwi1

Use

Tags

Expression

Mutation

Promoter

T7 sense, T3 antisense

Available since

April 2, 2012

Availability

Academic Institutions and Nonprofits only
pBS RAI14

Plasmid

#19204

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Retinoic acid induced 14 (RAI14 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pBS Olfactomedin1

Plasmid

#19225

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Olfactomedin 1 (OLFM1 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pBS WSB2

Plasmid

#19190

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

WSB2 (WSB2 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
fabp10a:DD-CreER; cryaa:mCerulean

Plasmid

#230076

Purpose

Expression of destabilized CreER in zebrafish hepatocytes.

Depositor

Sumeet Pal Singh

Article

Hovhannisyan et al bioRxiv 2025.05.23.655316

Insert

ltDHFR(DD)

Use

Zebrafish expression

Tags

CreER and HA

Expression

Mutation

amino acids mutations: R12H, N23S, G67S, V78A, E1…

Promoter

fabp10a

Available since

May 30, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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fabp10a:FLAG-SpiCee-mRFP1; cryaa:mCherry

Plasmid

#230062

Purpose

Expression of genetically encoded calcium chelator in zebrafish hepatocytes.

Depositor

Sumeet Pal Singh

Article

Pozo-Morales et al Hepatology. 2023 Mar 1;77(3):789-801. do

Insert

SpiCee

Use

Zebrafish expression

Tags

FLAG and mRFP1

Expression

Mutation

Promoter

fabp10a

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-giantin-ATG_donor

Plasmid

#222316

Purpose

Perform knock-in by homologous recombination in giantin/GOLGB1 gene.

Depositor

Franck Perez

Article

Fourriere et al J Cell Sci. 2016 Sep 1;129(17):3238-50.

Insert

EGFP and homology arms for giantin/GOLGB1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSsgRNA

Plasmid

#224867

Purpose

Yeast genome editing, Insertion of 20 bp oligo into sgRNA gene

Depositor

Katsunori Suzuki

Article

Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJBM2

Plasmid

#225289

Purpose

E. coli-Campylobacter shuttle vector; KanR

Depositor

Robert Moore

Article

McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

Insert

Replication elements from pCC311 for pJBM2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJBM1

Plasmid

#225288

Purpose

E. coli- C. coli shuttle vector; KanR

Depositor

Robert Moore

Article

McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

Insert

Replication elements from pWestmill 11

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGTag Hygro BFP nLAP +0

Plasmid

#194317

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

Insert

Hygro-P2A-nLAP(BFP)

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGTag Hygro EGFP nLAP -2

Plasmid

#194322

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

Insert

Hygro-P2A-nLAP(EGFP)

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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