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tet_pLKO.1_puro_shNRF2 #2

Plasmid

#136585

Purpose

Expresses an inducible short hairpin targeting human NRF2 sequence

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

shNRF2v2 (NFE2L2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

June 13, 2020

Availability

Academic Institutions and Nonprofits only

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pFG4

Plasmid

#87251

Purpose

gene for resistance to hygromycin (Hyg) inserted next to the parS segments (INT). Also known as pANCH2-Hph

Depositor

Kerstin Bystricky

Article

Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

Insert

Hyg

Tags

parS segments

Expression

Yeast

Promoter

TEF

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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Gi3-CASE

Plasmid

#168122

Purpose

Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

Depositor

Gunnar Schulte

Article

Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

Insert

GNAI2, GNB1, GNG2 (GNAI1, GNB1, GNG2 Human)

Use

Luciferase

Tags

cpVenus on GNG2

Expression

Mammalian

Mutation

NLuc is inserted at A114/E115 within GNAI1

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pBABE-puro LIP

Plasmid

#15713

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

LIP (CEBPB Human)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag SGK

Plasmid

#20628

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

SGK (SGK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-KLF5

Plasmid

#40918

Depositor

Jin-Tang Dong

Article

Zhao et al Biochem J. 2011 Jul 15;437(2):323-33.

Insert

Kruppel-like factor 5 (KLF5 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pCM184-ParB2::eGFP

Plasmid

#87252

Purpose

Expresses ParB2-EGFP in yeast. Also known as pOR2-GFP

Depositor

Kerstin Bystricky

Article

Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

Insert

ParB2

Tags

EGFP

Expression

Yeast

Promoter

pBAD/tetO

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MDH1-PGK-GFP microRNA-10b

Plasmid

#16070

Depositor

Bob Weinberg

Article

Ma et al Nature. 2007 Oct 11. 449(7163):682-8.

Insert

microRNA-10b (MIR10B Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag MAP3K7

Plasmid

#20520

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAP3K7 (MAP3K7 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Q356H

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pMSCV_IRES_GFP_CD16a

Plasmid

#196189

Purpose

Retroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.

Depositor

Ana Ruiz-Saenz

Article

Vincken et al STAR Protoc. 2023 Apr 17;4(2):102224. do

Insert

CD16a (FCGR3A Human)

Use

Retroviral

Expression

Mammalian

Mutation

changed Phenylalanine 158 to Valine

Promoter

LTR

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCM189-ParB1::mCherry

Plasmid

#87253

Purpose

Expresses ParB1-mCherry in yeast. Also known as pOR1-mch

Depositor

Kerstin Bystricky

Article

Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

Insert

ParB1

Tags

mCherry

Expression

Yeast

Promoter

pBAD/tetO

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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SAFB1-EGFP

Plasmid

#32746

Depositor

Steffi Oesterreich

Article

Townson et al Clin Cancer Res. 2000 Sep;6(9):3788-96.

Insert

SAFB1 (SAFB Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag BTK

Plasmid

#20432

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

BTK (BTK Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pBabe Angptl4 puro

Plasmid

#19156

Depositor

Joan Massague

Article

Padua et al Cell. 2008 Apr 4. 133(1):66-77.

Insert

angiopoietin-like 4 (ANGPTL4 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only
pBABE_hygro_mRFP1_NRF2

Plasmid

#136579

Purpose

mammalian expression of mRFP1-tagged human NRF2

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

NRF2 (NFE2L2 Human)

Use

Retroviral

Tags

N-mRFP1-FLAG

Expression

Mammalian

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pBabePuro-hSNAIL.ER.NoTag

Plasmid

#19292

Depositor

Eric Fearon

Article

Hajra et al Cancer Res. 2002 Mar 15. 62(6):1613-8.

Insert

SNAIL (SNAI1 Human)

Use

Retroviral

Tags

mouse ERalpha ligand-binding domain

Expression

Mammalian

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pLKO.1 shRUNX1 puro

Plasmid

#45816

Depositor

Kevin Janes

Article

Wang et al Proc Natl Acad Sci U S A. 2011 Oct 4;108

Insert

Runx1 (RUNX1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A RIGI WT

Plasmid

#167289

Purpose

Lentiviral expression construct encoding a TagBFP in frame with a self-cleaving wild-type RLR sensor RIG-I

Depositor

Agnel Sfeir

Article

Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

Insert

DDX58 (RIGI Human)

Use

Lentiviral

Tags

tagBFP

Expression

Mammalian

Promoter

SFFV

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223 NRF2

Plasmid

#118708

Purpose

entry clone for NRF2

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

NRF2

Expression

Mammalian

Mutation

wt

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEX.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP

Plasmid

#45565

Purpose

Expresses I-Sce I nuclease in mammalian cells and BFP as a tracker

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Inserts

I-Sce I Nuclease
BFP

Tags

HA

Expression

Mammalian

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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