We narrowed to 7,363 results for: PAC

1. pAcBac1-Ma-sfGFP WT

   Plasmid

   #197567

   Purpose

   Expression of sfGFP WT with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

   Depositor

   Ryan Mehl

   Article

   Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

   Inserts

   sfGFP WT
   M. alvus Pyl-tRNA (6) (4x copies)
   

   Use

   Tags

   V5-His6

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6/H1

   Available since

   April 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pActPL-VP16AD

   Plasmid

   #15305

   Depositor

   Benjamin White

   Article

   Luan et al Neuron. 2006 Nov 9. 52(3):425-36.

   Insert

   VP16AD-ZIP+
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 6, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCS2-mCherry_SNAP_SNAPV_PACT_Dead

   Plasmid

   #173073

   Purpose

   Dead SNAP tag at the centrosome

   Depositor

   Heidi Hehnly

   Article

   Bucko et al Elife. 2019 Dec 24;8. pii: 52220. doi: 1

   Insert

   mCherry-SNAP-SNAP-Varied-PACT
   

   Use

   Tags

   mCherry-SNAP-SNAP-Varied

   Expression

   Mammalian

   Mutation

   AA144 in both SNAP tags changed from a cysteine t…

   Promoter

   CMV IE94

   Available since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Epac-S-H94

   Plasmid

   #170341

   Purpose

   GFP(UST)_EPACdDEPCD_mCherry biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do

   Insert

   GFP(UST)_EPACdDEPCD_mCherry (RAPGEF3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. bPAC-mycHis_pGEM

   Plasmid

   #85468

   Purpose

   Expression of photoactivated cyclase bPAC of Beggiatoa sp.

   Depositor

   Peter Hegemann

   Article

   Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

   Insert

   Bacterial Photoactivated Adenylyl Cyclase
   

   Use

   Tags

   His and Myc

   Expression

   Mutation

   Promoter

   T7 promoter

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pACEC3GH (Apr)

   Plasmid

   #110085

   Purpose

   Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   3C cleavage site - GFP - His10 Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pActinin-miRFP670nano

   Plasmid

   #127432

   Purpose

   Alpha actinin labeling with near-infrared fluorescent protein

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

   Insert

   Alpha-actinin-miRFP670nano
   

   Use

   Tags

   miRFP670nano

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAcGFP1-N1-RvLEAM

   Plasmid

   #90035

   Purpose

   To express GFP-tagged RvLEAM in mammalian cells transiently

   Depositor

   Takekazu Kunieda

   Article

   Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

   Insert

   RvLEAM
   

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAc5.1B-MS2_V

   Plasmid

   #147737

   Purpose

   Insect Expression vector

   Depositor

   Elisa Izaurralde

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFGC5941-PacI

    Plasmid

    #44020

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAcGFP1-N1-CAHS3

    Plasmid

    #90031

    Purpose

    To express GFP-tagged CAHS3 in mammalian cells transiently

    Depositor

    Takekazu Kunieda

    Article

    Yamaguchi et al PLoS One. 2012;7(8):e44209.

    Insert

    CAHS3
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAC-mCherry2-G4

    Plasmid

    #164167

    Purpose

    Expresses mCherry2 under the control of CMV promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal

    Depositor

    Isei Tanida

    Article

    Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

    Insert

    mCherry2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMC060-HisMS2_PLP_Env_pac

    Plasmid

    #155040

    Purpose

    Generation of MS2 Virus Like Particles packaged with the sequence for the SARS-CoV-2 Envelope Gene

    Depositor

    Paul Freemont

    Article

    Crone et al Nat Commun. 2020 Sep 8;11(1):4464. doi:

    Inserts

    Maturation Protein
    Coat Protein Dimer
    Envelope Gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Remove TypeIIs Restriction Sites

    Promoter

    T7

    Available since

    Sept. 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pAC3-ISH

    Plasmid

    #15854

    Depositor

    Peter Mombaerts

    Article

    Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

    Insert

    Adenylate Cyclase 3 (Adcy3 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    contains residues 787-1021

    Promoter

    Available since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

15. L40C-CRISPR.EFS.PAC

    Plasmid

    #89393

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), PAC (Puromycin resistance) coexpression, EFS Promoter driven

    Depositor

    Dirk Heckl

    Article

    Reimer et al Haematologica. 2017 Jun 1. pii: haematol

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAcGFP1-N1-CAHS1

    Plasmid

    #90030

    Purpose

    To express GFP-tagged CAHS1 in mammalian cells transiently

    Depositor

    Takekazu Kunieda

    Article

    Yamaguchi et al PLoS One. 2012;7(8):e44209.

    Insert

    CAHS1
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAC32 [TU#839]

    Plasmid

    #16079

    Depositor

    Martin Chalfie

    Insert

    mec-2 intron 9
    

    Use

    Tags

    YFP

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pActinin-TagRFP675-N1

    Plasmid

    #44278

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Sci Rep. 2013;3:1847. doi: 10.1038/srep0

    Insert

    TagRFP675
    

    Use

    Tags

    Actinin

    Expression

    Mammalian

    Mutation

    M41Q/F80W/S143N/L147M/S158N/D159Y/N173S

    Promoter

    CMV

    Available since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAC1819_pCR8-DR-SMN2-2-DR

    Plasmid

    #118653

    Purpose

    Transient transfection; Expressed DR-SMN2-DN2-DR CasRx gRNA with preprocessed DR 3'; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    DR-SMN2-2-DR
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAct-dCBEevoAPOBEC1

    Plasmid

    #195514

    Purpose

    Ubiquitous expression of dCBEevoAPOBEC1 in Drosophila

    Depositor

    Michael Boutros

    Article

    Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

    Insert

    dCBE-evoAPOBEC1
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    act5C

    Available since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge