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pET28b-PU.1-ETS

Plasmid

#85734

Purpose

Expresses DNA-binding (ETS) domain of murine PU.1, residues 167-272

Depositor

Gregory Poon

Article

Wang et al J Biol Chem. 2014 Aug 1;289(31):21605-16

Insert

ETS domain of murine PU.1 (residues 167-272) (Spi1 Mouse, Human)

Use

Tags

6xHis following thrombin cleavage site LVPR|G

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pISH-Ascl1

Plasmid

#74338

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Ascl1 (also known as Mash1). Not for gene expression.

Depositor

Peter Mombaerts

Article

Hirota et al Proc Natl Acad Sci U S A. 2004 Jun 8;101

Insert

achaete-scute family bHLH transcription factor 1 (Ascl1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-nAChr-Alpha6

Plasmid

#48812

Purpose

this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

Depositor

Henry Lester

Article

Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

Insert

nAChr-alpha6 (Chrna6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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sgCRY1

Plasmid

#176511

Purpose

Expresses sgRNA in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgCRY1-2 (Cry1, Cry2 Mouse)

Use

AAV

Tags

mCherry (K14E, N210D, E249G)

Expression

Mutation

Promoter

U6, hSyn

Available since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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MDH1-miR-31SM-PGK-GFP-Blast

Plasmid

#31516

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-31 (Mir31 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

seed mutant control

Promoter

Available since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only
MEIS1B-MSCV

Plasmid

#21022

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 1999 Apr . 19(4):3051-61.

Insert

MEIS1B (Meis1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-nMyc-wt_3'UTR

Plasmid

#31858

Depositor

Robert Blelloch

Article

Melton et al Nature. 2010 Feb 4;463(7281):621-6. Epub

Insert

nMyc wild-type 3'UTR

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Zbtb46

Plasmid

#139811

Purpose

doxycycline-inducible expression of mouse Zbtb46 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Zbtb46 (Zbtb46 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE-mCMV

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pF(UG) CamKII sf iGluSnFR

Plasmid

#158769

Purpose

iGluSnFR glutamate sensor containing a Golgi export sequence and and an ER exit motif for improved trafficking to the plasma membrane.

Depositor

Edwin Chapman

Article

Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

Insert

iGluSnFR

Use

Lentiviral

Tags

Expression

Mutation

Additional ER and Golgi export sequences

Promoter

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1 Srap1

Plasmid

#89691

Purpose

Expresses murine Srap1 in bacteria

Depositor

Douglas Feldman

Article

Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

Insert

Murine SRAP domain ortholog Srap1 (Hmces Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pRDAX-396 FLAG UnaG[1] Stuffer/InteinA UnaG[2]

Plasmid

#130144

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

UnaG

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-Nav1 Na+ channel [N419/78R]

Plasmid

#114553

Purpose

Mammalian Expression Plasmid of anti-Pan-Nav1 Na+ channel (Human). Derived from hybridoma N419/78.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pan-Nav1 Na+ channel (Homo sapiens) recombinant mouse monoclonal antibody (SCN5A Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-ms-c-Myc-V394D

Plasmid

#50775

Purpose

retroviral expression of mouse c-Myc V394D mutant

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Jul 26. (

Insert

c-Myc V394D (Myc Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

V394D (does not bind to Miz-1)

Promoter

Available since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pJSTX-GL3

Plasmid

#11982

Purpose

Reporter gene with mouse c-jun promoter and point mutation of MEF2 and AP1 binding sites

Depositor

Ron Prywes

Article

Clarke et al Mol Cell Biol. 1998 Feb . 18(2):1065-73.

Insert

c-jun promoter (Jun Mouse)

Use

Luciferase

Tags

Luc

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
Anti-HCN4 [N114/10.1R]

Plasmid

#114506

Purpose

Mammalian Expression Plasmid of anti-HCN4 (Human). Derived from hybridoma N114/10.1.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-HCN4 (Homo sapiens) recombinant mouse monoclonal antibody (HCN4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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HIPK1-myc-his

Plasmid

#1850

Depositor

Philip Leder

Article

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

HIPK1 (Hipk1 Mouse)

Use

Tags

his and myc

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2005

Availability

Academic Institutions and Nonprofits only
DN9

Plasmid

#21209

Depositor

Paul Khavari

Article

Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

Insert

Mek1 (Esr1, MAP2K1 Mouse, Human)

Use

Subclone vector

Tags

Expression

Mutation

Mek1 K97M fused to ER*, catalytically inactive

Promoter

Available since

July 24, 2009

Availability

Academic Institutions and Nonprofits only
Pinco-mNrp1dCy

Plasmid

#21936

Depositor

Guido Serini

Article

Valdembri et al PLoS Biol. 2009 Jan 27. 7(1):e25.

Insert

neuropilin 1 (Nrp1 Mouse)

Use

Retroviral

Tags

HA and His

Expression

Mutation

deleted amino acids 884-923

Promoter

Available since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only
pEGFP-RapR-FAK-YM-Y397F

Plasmid

#25930

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jun 27. ():.

Insert

RapR-FAK (Ptk2 Mouse, Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

This pEGFP-RapR-FAK-YM-Y397F plasmid contains the…

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pEGFP-RNF17S

Plasmid

#35414

Depositor

Jeremy Wang

Article

Pan et al Development. 2005 Sep;132(18):4029-39. E

Insert

Rnf17s (Rnf17 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 25, 2013

Availability

Academic Institutions and Nonprofits only
pJSX-GL3

Plasmid

#11980

Purpose

Reporter gene with mouse c-jun promoter and point mutation of MEF2 binding site

Depositor

Ron Prywes

Article

Clarke et al Mol Cell Biol. 1998 Feb . 18(2):1065-73.

Insert

c-jun promoter (Jun Mouse)

Use

Luciferase

Tags

Luc

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pMMP-puro-Flag-HA-NR6A1

Plasmid

#46833

Depositor

Phil Sharp

Article

Gurtan et al Genes Dev. 2013 Apr 15;27(8):941-54. doi

Insert

NR6A1 (Nr6a1 Mouse)

Use

Retroviral

Tags

Flag-HA, plus 8-amino acid residue linker

Expression

Mammalian

Mutation

None

Promoter

Available since

Nov. 25, 2013

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Zfp36-3'UTR

Plasmid

#61780

Purpose

Luciferase reporter containing the 3'UTR of mouse Zfp36

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Zfp36 3'UTR

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pCLEN

Plasmid

#17704

Depositor

Nicholas Gaiano

Article

Gaiano et al Neuron. 2000 May . 26(2):395-404.

Insert

NIC (Notch1 Mouse)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2008

Availability

Academic Institutions and Nonprofits only
HMGA2 shRNA

Plasmid

#25408

Depositor

Sean Morrison

Article

Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

Insert

high mobility group AT-Hook 2 (Hmga2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
Anti-Calbindin [L109/62R]

Plasmid

#188187

Purpose

Mammalian Expression Plasmid of anti-Calbindin (Human). Derived from hybridoma L109/62

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Calbindin (Homo sapiens) recombinant mouse monoclonal antibody (CALB1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCLC-mEos2

Plasmid

#38010

Depositor

Xiaowei Zhuang

Article

Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

Insert

Clathrin, light polypeptide (LCa) (Clta Mouse)

Use

Tags

mEos2

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Sept. 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
mEmerald-MKS1-N-18

Plasmid

#54183

Purpose

Localization: Cilium, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

MKS1

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
tdTomato-CAF1-C-10

Plasmid

#58072

Purpose

Localization: Chromatin Assembly Factor 1, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Insert

CAF1

Use

Tags

tdTomato

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6p-Meis1a

Plasmid

#78144

Purpose

bacterial expression of GST-Meis1a

Depositor

Francesco Blasi

Article

Dardaei et al Proc Natl Acad Sci U S A. 2014 Mar 11;11

Insert

Meis1a (Meis1 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

June 10, 2016

Availability

Academic Institutions and Nonprofits only