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p-att-ef1a-ABE8e-dCas9-BSD

Plasmid

#226116

Purpose

Using promoter ef1a expresses ABE8e-dCas9 protein with Blasticidin selection

Depositor

George Church

Article

Oliynyk et al Commun Biol. 2025 Dec 16;8(1):1778. doi:

Insert

BSD

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pHCMV_GP64

Plasmid

#207276

Purpose

Mammalian expression of baculovirus GP64 protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

GP64

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (D210N)

Plasmid

#196703

Purpose

Plasmid for transient mammalian expression of catalytically inactive RNase H1 mutant.

Depositor

Karlene Cimprich

Article

Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

Insert

human RNaseH1 D210N

Expression

Mammalian

Mutation

D210N, catalytically inactive

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLBH531_MBP-Cas14a1 expression

Plasmid

#112500

Purpose

Expresses MBP fused to Cas14a1

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14a1

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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phU1-CasRx-non-targeting (pKW82)

Plasmid

#229050

Purpose

Cloning backbone for CasRx crRNA (Human U1 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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GPRC5B-Tango

Plasmid

#66383

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

GPRC5B (GPRC5B Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pPB-EF1α-H2B-HaloTag-IRES-puro

Plasmid

#247460

Purpose

Piggybac based mammalian expression vector for histone H2B HaloTag, driven by the EF1a promoter.

Depositor

Kazuhiro Maeshima

Article

Hibino et al Nat Commun. 2024 Aug 21;15(1):7152. doi:

Insert

H2B (H2BC11) (H2BC11 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-SQSTM1-WT

Plasmid

#194582

Purpose

Mammalian Expression of mEGFP-SQSTM1 WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

SQSTM1 (SQSTM1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pTG612

Plasmid

#237387

Purpose

mStayGold^NSEC^ repair template

Depositor

Ariel Pani

Article

Gibney et al Genetics. 2026 Jan 20:iyag016. doi: 10.1

Insert

mStayGold^NSEC^

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-IRES-Blast

Plasmid

#85133

Purpose

lentiviral backbone

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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