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We narrowed to 14,107 results for: CAN

Showing: 1821 - 1830 of 14107 results
  1. pDG330

    Plasmid
    #100898
    Purpose
    SpCas9 with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
  2. pBA-mKeima-PIM

    Plasmid
    #174650
    Purpose
    Low level expression of mKeima-PIM. Can be used for ratiometric detection of inducible aggregate acidification after addition of AP20187. mKeima fluorophore allows for simultaneous GFP-tagged imaging.
    Depositor
    Insert
    xXFKBP homo-mKeima-2x FKBP homo-2x FKBP
    Expression
    Mammalian
    Mutation
    Val24Glu, Tyr80Cys, Ala95Thr mutations in second …
    Promoter
    Chicken beta-actin
    Available Since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. dCas9-NS3-NLS/VPR

    Plasmid
    #112244
    Purpose
    Encodes the drug-preservable Cas9-NS3-NLS/VPR, which can be used to activate gene expression when combined with an NS3 inhibitor and appropriately designed sgRNA.
    Depositor
    Insert
    dCas9-NS3-NLS/VPR
    Tags
    AU1 (N term on NS3) and HA (C term on NS3)
    Expression
    Mammalian
    Mutation
    NS3 mutant T54A
    Promoter
    CMV
    Available Since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. pCAP03-ermE*p

    Plasmid
    #120234
    Purpose
    Carries promoter cassette containing ermE*p promoter and ampicillin resistance gene that can be amplified with homology sequence for refactoring.
    Depositor
    Insert
    promoter cassette containing ermE*p promoter and ampicillin resistance gene
    Use
    Synthetic Biology
    Mutation
    bla (amp resistance gene)
    Available Since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. pDG335

    Plasmid
    #100899
    Purpose
    SpCas9n (D10A Nickase mutant) with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 1821 - 1830 of 14107 results