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pCMV-BE3

Plasmid

#73021

Purpose

Expresses BE3 in mammalian cells

Depositor

David Liu

Article

Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

Insert

BE3

Expression

Mammalian

Promoter

CMV

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pMV306G13+Lux

Plasmid

#26160

Depositor

Brian Robertson, Siouxsie Wiles

Article

Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Insert

G13 promoter + Bacterial luciferase operon

Expression

Bacterial

Mutation

It contains a gram-positive enhanced translation …

Available Since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only

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V5-miniTurbo-NES_pCDNA3

Plasmid

#107170

Purpose

expresses V5-tagged miniTurbo in the mammalian cytosol

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurbo (BirA mutant)

Expression

Mammalian

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_omicron BA.2

Plasmid

#183700

Purpose

expressing SARS-CoV-2 BA.2 spike protein for pseudovirus production

Depositor

David Nemazee

Article

Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

Insert

Spike (BA.2) (S SARS-CoV-2)

Use

Lentiviral and Retroviral

Expression

Mammalian

Mutation

c-terminal 18 aa deletion

Promoter

CMV

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-ER GFP

Plasmid

#80069

Purpose

To express and target GFP into the endoplasmic reticulum using lentivirus

Depositor

Pantelis Tsoulfas

Insert

ER GFP (SEC61B Human)

Use

Lentiviral

Tags

GFP derived from Aequorea coerulescens on the NH2…

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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mEmerald-Mito-7

Plasmid

#54160

Purpose

Localization: Mitochondria, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

Insert

Mito (COX8A Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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AiP20027 - pAAV-AiE2182m_3xC2-minBG-iCre(R297T)-BGHpA

Plasmid

#220735

Purpose

AiE2182m_3xC2 is an optimized enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minBG

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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CTCF-C CRISPR

Plasmid

#140647

Purpose

CTCF tagging CRISPR

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

CTCF (CTCF Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pLX_311-KRAB-dCas9

Plasmid

#96918

Purpose

for CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121

Depositor

John Doench, William Hahn, David Root

Article

Rosenbluh et al Nat Commun. 2017 May 23;8:15403. doi: 10

Insert

dCas9

Use

Lentiviral

Tags

KRAB

Expression

Mammalian

Mutation

dCas9

Promoter

EF1a

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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c-Flag pcDNA3

Plasmid

#20011

Purpose

This pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.

Depositor

Stephen Smale

Article

Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-mTurquoise2

Plasmid

#98886

Purpose

Produces a fusion between mTurquoise2 and mNeonGreen. It can be used as a positive control for FRET

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

mNeonGreen-mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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AAV.TBG.PI.Cre.rBG

Plasmid

#107787

Purpose

AAV vector expressing Cre from TBG promoter

Depositor

James M. Wilson

Has Service

AAV8

Insert

Cre

Use

AAV

Expression

Mammalian

Promoter

TBG

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV_BE4max_P2A_GFP

Plasmid

#112099

Purpose

C:G-to-T:A base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

BE4max_P2A_GFP

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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PB-CRISPR

Plasmid

#160047

Purpose

For cloning sgRNA to knockout a specific gene in piggybac plasmid

Depositor

Xiaojun Lian

Article

Jiang et al Bioact Mater. 2022 Feb 7;14:313-320. doi

Type

Empty backbone

Use

CRISPR; Piggybac

Expression

Mammalian

Promoter

EFS

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMD2.G-K47Q/R354Q

Plasmid

#207323

Purpose

VSV-G mutant with abolished receptor binding, but maintained fusogenic capacity

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

VSV-G (VSIVgp4 Vesicular Stomatitis Virus)

Expression

Mammalian

Mutation

K47Q/R354Q

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAXY0002

Plasmid

#179835

Purpose

Binary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoter

Depositor

Xiaohan Yang

Article

Yuan et al Hortic Res. 2021 Nov 1;8(1):234. doi: 10

Type

Empty backbone

Use

Binary vector for plant transformation

Expression

Plant

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Cre

Plasmid

#123133

Purpose

Mammalian expression vector for wild-type Cre.

Depositor

David Liu

Article

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

Cre recombinase

Expression

Mammalian

Promoter

CMV

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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RG6

Plasmid

#80167

Purpose

fluorescent protein splicing reporter

Depositor

Thomas Cooper

Article

Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

Insert

dsRed and GFP

Expression

Mammalian

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pSBbi 41BBL-CD86 mIL-15

Plasmid

#183245

Purpose

Expression of human 41BBL, CD86, and membrane bound human IL-15 (IL-15-IL-15Ra) in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

Depositor

Alexander McLellan

Article

Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

Insert

41BBL, CD86, mbIL-15 (IL-15-IL-15Ra)

Expression

Mammalian

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_TRE3GS-->mCherry_PGK-->BFP

Plasmid

#162231

Purpose

Lentiviral vector for the expression of TRE3GS-->mCherry_PGK-->BFP in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

mCherry and BFP

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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