We narrowed to 9,644 results for: SUB

1. pCMV-PE2-VRER

   Plasmid

   #159984

   Purpose

   Prime editing in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

   Insert

   PE2-VRER
   

   Tags

   BpSV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBMN-FKBP DD(L106P)-UbK0G75C-sfGFP

   Plasmid

   #172001

   Purpose

   Expression for LIBRON constructs

   Depositor

   Thomas Wandless

   Article

   Miyamae et al Cell Chem Biol. 2020 Dec 17;27(12):1573-

   Insert

   FKBP DD (L106P)-UbK0G75C
   

   Use

   Retroviral

   Tags

   sfGFP

   Mutation

   change all lysin residues of ubiquitin to Arg, G7…

   Available Since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. mito-mGarnet

   Plasmid

   #104309

   Purpose

   codes for a fusion of fluorescent protein mGarnet and mito, stains mictochondrial content in live cells, i.e. human cytochrome c oxidase subunit VIII, the mitochondrial targeting sequence mito

   Depositor

   Ulrich Nienhaus

   Article

   Hense et al Sci Rep. 2015 Dec 9;5:18006. doi: 10.103

   Insert

   mito-mGarnet
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Flag-HA-EIF3S5

   Plasmid

   #22546

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   EIF3S5 (EIF3F Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Nov. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-N1-mt-ro1GFP

   Plasmid

   #82407

   Purpose

   Expression of redox sensitive GFP with a mitochondrial targeting sequence in mammalian cells

   Depositor

   S. James Remington

   Article

   Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

   Insert

   ro1GFP
   

   Tags

   leader sequence of the E1α subunit of pyruvate de…

   Expression

   Mammalian

   Mutation

   C48S/F64L/T65S/S147C/Q204C

   Promoter

   CMV

   Available Since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-ABKAR(T/A)

   Plasmid

   #140052

   Purpose

   Negative control mutant for ABKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

   Insert

   ABKAR(T/A)
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available Since

   Nov. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQTEV-PPP1R1A

   Plasmid

   #31331

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   protein phosphatase 1 (PPP1R1AP1 Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   Nov. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV3Tag8li-hSAPS1-HA

   Plasmid

   #165466

   Purpose

   Human SAPS1 mammalian expression vector with C-terminal HA tag.

   Depositor

   Martin Dorf

   Article

   Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

   Insert

   Protein phosphatase 6 regulatory subunit 1 (PPP6R1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB1H2w2-mutOdd

   Plasmid

   #18044

   Depositor

   Scot Wolfe

   Article

   Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

   Insert

   mutant Odd Skipped
   

   Expression

   Bacterial

   Mutation

   A mutant version of the zinc finger protein Odd S…

   Available Since

   June 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDM4-GLY

    Plasmid

    #49808

    Purpose

    Encodes pgk, gapA, aceE, aceF, lpdA in pseudo-operon configuration.

    Depositor

    Mattheos Koffas

    Article

    Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

    Inserts

    phosphoglycerate kinase
    glyceraldehyde-3-phosphate dehydrogenase
    pyruvate dehydrogenase subunit E1
    dihydrolipoamide acetyltransferase
    dihydrolipoamide dehydrogenase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. Cmv d0 dPspCas13b-GGS-NYTHDF1(100-200)

    Plasmid

    #119857

    Purpose

    Cmv d0 dPspCas13b-GGS-NYTHDF1(100-200) (YTHDF1 100-200 subdomain)

    Depositor

    Bryan Dickinson

    Article

    Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

    Insert

    dCas13b-GGS-NYTHDF1(100-200)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cmv d0 dPspCas13b-GGS-NYTHDF2(100-200)

    Plasmid

    #119856

    Purpose

    Cmv d0 dPspCas13b-GGS-NYTHDF2(100-200) (YTDHF2 100-200 subdomain)

    Depositor

    Bryan Dickinson

    Article

    Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

    Insert

    dCas13b-GGS-NYTHDF2(100-200)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAd-PV-NLS-CD-DsRed

    Plasmid

    #16345

    Depositor

    Anton Bennett

    Article

    Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

    Insert

    PV-DsRed-NLS-CD (Pvalb Rat)
    

    Use

    Adenoviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Functional Ca2+binding site CD was inactivated by…

    Available Since

    Feb. 26, 2008

    Availability

    Academic Institutions and Nonprofits only

14. Expression scaffold for dCas9+sgRNA

    Plasmid

    #171799

    Purpose

    PICASSO: Expression scaffold for second subcloning step to properly co-express paired dCas9-fusion+sgRNA

    Depositor

    Stephen Elledge

    Article

    Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

    Insert

    PICASSO: expression scaffold for proper dCas9-fusion + sgRNA expression
    

    Use

    CRISPR

    Available Since

    Aug. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmRFP-SUMO1 GG

    Plasmid

    #13990

    Depositor

    Jonathan Chernoff

    Article

    Dadke et al Nat Cell Biol. 2007 Jan . 9(1):80-85.

    Insert

    SUMO1 GG (Sumo1 Mouse)
    

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    C-terminal truncation. Cannot ligate to substrate…

    Available Since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

16. IBMc460

    Plasmid

    #161940

    Purpose

    Carries Golden Gate substitution insert for IBM with gp41-1 intein. PPhlF as C-lobe promoter

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1A3-BbsI-GY-gp-41-1N-cat-PPhlF-RiboJ-B32-gp41-1C-SS-SapI
    

    Expression

    Bacterial

    Promoter

    PPhlF

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. PKAcs-GGS4X-GFP

    Plasmid

    #108583

    Purpose

    Expresses PKAcs-GFP fusion in mammalian cells with flexible linker

    Depositor

    Robert Hughes

    Article

    Norris et al FEBS Open Bio. 2018 Apr 25;8(6):1029-104

    Insert

    protein kinase A, catalytic subunit
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    H87Q and W196R

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-104

    Plasmid

    #79842

    Purpose

    Expresses NLS-PpsR2-VP16 under CMV promoter

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

    Insert

    NLS-PpsR2-VP16
    

    Tags

    Nuclear localization signal of nuclear cap-bindin…

    Expression

    Mammalian

    Mutation

    RpPpsR2 cysteine 439 changed to serine

    Promoter

    CMV

    Available Since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pY2

    Plasmid

    #218115

    Purpose

    Activity reporter backbone with an empty protease cassette (BsaI) under (lexA-box)3PminCYC1 promoter and an empty substrate cassette (BsmBI) under pGAL1 promoter.

    Depositor

    Carl Denard

    Article

    Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-S9

    Plasmid

    #132948

    Purpose

    Expresses N-terminal 6xHis-tagged S9 ribosomal protein in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    30S ribosomal subunit protein S9 (rpsI E.coli)
    

    Tags

    N-terminal 6xHis-tag

    Expression

    Bacterial

    Mutation

    E52C

    Promoter

    T7

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pVimentin-LSSmOrange

    Plasmid

    #37134

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

    Insert

    LSSmOrange
    

    Tags

    human cytochrome C oxidase subunit VIII and human…

    Expression

    Mammalian

    Mutation

    A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

    Promoter

    CMV

    Available Since

    June 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEGFP-N1-mt-ro2GFP

    Plasmid

    #82408

    Purpose

    Expression of redox sensitive GFP with a mitochondrial targeting sequence in mammalian cells

    Depositor

    S. James Remington

    Article

    Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

    Insert

    ro2GFP
    

    Tags

    leader sequence of the E1α subunit of pyruvate de…

    Expression

    Mammalian

    Mutation

    C48S/F64L/S147C/Q204C

    Promoter

    CMV

    Available Since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBS-L1PA8CHmneo

    Plasmid

    #69608

    Purpose

    Expresses a neomycin tagged codon optimized L1PA8

    Depositor

    Astrid Roy-Engel

    Article

    Wagstaff et al Mol Biol Evol. 2013 Jan;30(1):88-99. doi

    Insert

    L1PA8
    

    Tags

    mneo retrotransposition cassette

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pQLinkHD_EBFP2-LSSmOrange

    Plasmid

    #118856

    Purpose

    fluorescent protein expression with an N-terminal His-tag

    Depositor

    Marc Birtwistle

    Article

    Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

    Insert

    EBFP2-LSSmOrange
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    EBFP1.2-I128V/V150I/ D155V/V224R and for LSSmOra…

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. FCIV1-GR

    Plasmid

    #72701

    Purpose

    Lentiviral expression of rat GR (2nd generation)

    Depositor

    Freddy Jeanneteau

    Article

    Lambert et al Mol Cell Biol. 2013 Sep;33(18):3700-14.

    Insert

    GR (Nr3c1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-Venus

    Expression

    Mammalian

    Promoter

    ubiquitin

    Available Since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

26. pBT344_pattB-pCA-GFP-pA-FRT5

    Plasmid

    #52550

    Purpose

    for Phic31 integrase-mediated transgene integration;the bacterial backbone can be subsequently removed by crossing to Flp-expressing mice

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attB (full length)
    GFP
    

    Expression

    Mammalian

    Available Since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBS-L1PA8WTmneo

    Plasmid

    #69609

    Purpose

    Expresses a neomycin tagged wildtype L1PA8

    Depositor

    Astrid Roy-Engel

    Article

    Wagstaff et al Mol Biol Evol. 2013 Jan;30(1):88-99. doi

    Insert

    L1PA8
    

    Tags

    mneo retrotransposition cassette

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. KG#371

    Plasmid

    #110882

    Purpose

    Expresses the lysosome marker CTNS-1-mCherry cDNA in a subset of ventral cord cholinergic motor neurons

    Depositor

    Kenneth Miller

    Article

    Edwards et al Genetics. 2013 May;194(1):143-61. doi: 1

    Inserts

    unc-129 promoter
    CTNS-1a-mCherry cDNA
    unc-54 3' control region with 1 intron just upstream and 1 intron in control region
    

    Tags

    intron-mCherry

    Expression

    Bacterial

    Available Since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. TRAPa-3xFLAG-pCDNA

    Plasmid

    #201963

    Purpose

    Mammalian expression of Translocon-associated protein subunit alpha

    Depositor

    Ville Paavilainen

    Article

    Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

    Insert

    SSR1 (SSR1 Human)
    

    Tags

    3xFLAG-tag

    Expression

    Mammalian

    Available Since

    Jan. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Prohibitin-bio-His

    Plasmid

    #50800

    Purpose

    Expresses enzymatically monobiotinylated full length Prohibitin

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    Prohibitin
    

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pB194

    Plasmid

    #61154

    Purpose

    Expression of KIN28 under GAL1 promoter in yeast

    Depositor

    Charles Brenner

    Article

    Bieganowski et al J Biol Chem. 2002 Mar 29;277(13):10852-6

    Insert

    TFIIH complex serine/threonine-protein kinase subunit KIN28 (KIN28 Budding Yeast)
    

    Expression

    Yeast

    Promoter

    Gal1

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

32. fADL-1e-flag-linker-p3

    Plasmid

    #139441

    Purpose

    For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage.

    Depositor

    Alexander Gabibov

    Article

    Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

    Insert

    linker with pelB-leader, cloning site, flag-tag and p3
    

    Expression

    Bacterial

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMV-MTS-FLAG-LOV*

    Plasmid

    #202207

    Purpose

    Expresses the photoproximity labeling catalyst LOV* fused to the mitochondria targeting sequence (MTS) in mammalian cells

    Depositor

    Thomas Muir

    Article

    Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

    Insert

    Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, aa 1-24
    

    Tags

    FLAG and LOV*

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBS-L1PA4CHmneo

    Plasmid

    #69607

    Purpose

    Expresses a neomycin tagged codon optimized L1PA4

    Depositor

    Astrid Roy-Engel

    Article

    Wagstaff et al Mol Biol Evol. 2013 Jan;30(1):88-99. doi

    Insert

    L1PA4
    

    Tags

    mneo retrotransposition cassette

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3-HA-APC11

    Plasmid

    #19898

    Depositor

    Yue Xiong

    Article

    Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

    Insert

    APC11 (ANAPC11 Human)
    

    Tags

    HA and T7

    Expression

    Mammalian

    Available Since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

36. pGas-miRFP670nano3

    Plasmid

    #184672

    Purpose

    G protein alpha-subunit internally tagged with miRFP670nano3

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    G protein alpha-s-miRFP670nano3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. fADL-1e-HA-linker-p3

    Plasmid

    #139440

    Purpose

    For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage

    Depositor

    Alexander Gabibov

    Article

    Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

    Insert

    linker with pelB-leader, cloning site, HA-tag and p3
    

    Expression

    Bacterial

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pYIAL31

    Plasmid

    #60807

    Purpose

    used to make mutations in yeast POL1

    Depositor

    Thomas Kunkel, Youri Pavlov

    Article

    Pavlov et al Genetics. 2001 Sep;159(1):47-64.

    Insert

    Pol1 (POL1 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    N-terminal part only

    Available Since

    Feb. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. IBMc233

    Plasmid

    #161939

    Purpose

    Carries Golden Gate substitution insert for IBM with M86 intein. PPhlF as C-lobe promoter

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1A3-BbsI-M86N-T-cat-PPhlF-RiboJ-B32-M86C-SapI
    

    Expression

    Bacterial

    Promoter

    PPhlF

    Available Since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. SERA1-bio-His

    Plasmid

    #50812

    Purpose

    Expresses enzymatically monobiotinylated full length SERA1

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    SERA1
    

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge