We narrowed to 14,039 results for: TIM

1. pBAD-SCW

   Plasmid

   #190626

   Purpose

   Bacterial expression of genetically encoded calcium indicator SCW

   Depositor

   Robert Campbell

   Article

   Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

   Insert

   SCW
   

   Expression

   Bacterial

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJL2

   Plasmid

   #184130

   Purpose

   For rescuing null mrp-1 mutation and labelling mrp-1 protein. Translational fusion construct. pPD95.75_Pmrp-1::mrp-1(isoform C cDNA)::gfp.

   Depositor

   Alejandro Aballay

   Article

   Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 1

   Insert

   MRP-1 (mrp-1 Nematode)
   

   Tags

   GFP

   Expression

   Worm

   Promoter

   Pmrp-1

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJL1

   Plasmid

   #184129

   Purpose

   For rescuing null mrp-1 mutation. pPD95.77_Pmrp-1::mrp-1(isoform C cDNA)::SL2::gfp. SL2 prevents fusion of MRP-1 and GFP.

   Depositor

   Alejandro Aballay

   Article

   Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 1

   Insert

   MRP-1 (mrp-1 Nematode)
   

   Tags

   GFP

   Expression

   Worm

   Promoter

   Pmrp-1 (3kb)

   Available Since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. DYNLT1

   Plasmid

   #132528

   Purpose

   Human TCTEX-1, codon optimised for Sf9 expression.

   Depositor

   Andrew Carter

   Article

   Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

   Insert

   DYNLT1 (DYNLT1 Human)
   

   Expression

   Insect

   Mutation

   Codon optimised for Sf9 expression

   Available Since

   Oct. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. ShuttleMCP

   Plasmid

   #50959

   Purpose

   A backbone for adenovirus creation by the AdEasy method, similar to pShuttle-CMV (Addgene #16403), except it uses a mouse cofilin promoter (MCP) instead of the CMV promoter

   Depositor

   James Bamburg

   Article

   Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:

   Type

   Empty backbone

   Use

   Adenoviral

   Expression

   Mammalian

   Promoter

   uses portion of the mouse cofilin gene as a mouse…

   Available Since

   Feb. 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. LITE1.0_pAAV_hSyn_CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA

   Plasmid

   #48253

   Purpose

   LITE1.0 CRY2PHR fused to VP64 transcriptional activator domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression. See LITE2.0 for optimized LITE activators.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

   Insert

   CRY2PHR-NLS-VP64
   

   Use

   AAV; Tale

   Tags

   2A_GFP

   Expression

   Mammalian

   Promoter

   EF1-alpha

   Available Since

   Oct. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWL502-PAM

   Plasmid

   #174382

   Purpose

   Plasmids bearing protospacer sequences with functional PAM efficiently triggered CRISPR-mediated defense in cells with corresponding spacer sequence; article demonstrates use in Haloferax mediterranei

   Depositor

   Jing Han

   Article

   Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

   Insert

   PAM-spacer
   

   Use

   CRISPR; Archaeal expression

   Expression

   Bacterial

   Available Since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. ERroGFP_C147S_pCDNA3

   Plasmid

   #48630

   Purpose

   Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFP with Cysteine 147 to Serine mutation (roGFP C147S, C204)

   Depositor

   David Ron

   Article

   Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

   Insert

   ERroGFP
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   C147S

   Available Since

   Oct. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. MPC11 IgG2b gene

   Plasmid

   #127248

   Purpose

   Expresses intact IgG2b gene in mammalian cells

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   IgG2b gene with exons and alt pAs (Ighg2b Mouse)
   

   Expression

   Mammalian

   Available Since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCNL-N1_Mito

    Plasmid

    #65670

    Purpose

    Expresses Mito-CNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    cytochrome c oxidase subunit VIII
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTrichoGate-13

    Plasmid

    #160713

    Purpose

    For C-terminal tags with Trichoderma optimised Synthetic 3X Hemagglutinin tag (HA) bordered by BsaI restriction sites

    Depositor

    Artemio Mendoza

    Article

    Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

    Insert

    Trichoderma optimised Synthetic 3X Hemagglutinin tag (HA)
    

    Use

    Synthetic Biology

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pN1-mCherry-NKD2

    Plasmid

    #176641

    Purpose

    Expresses NKD2-mCherry fusion protein in mammalian cells

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    

    Tags

    mCherry tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. ERrefGFP1_pCDNA3

    Plasmid

    #48635

    Purpose

    Mam vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with mut Cys147 to Ser, del insertion147b and mut Cys204 to Glut.Referred to as ref GFP

    Depositor

    David Ron

    Article

    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

    Insert

    ERrefGFP1
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    C147S,C204Q

    Available Since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVMG0303_Cq-U6-6_2xBbsI_gRNA-Loop

    Plasmid

    #169370

    Purpose

    Expression of gRNA in Culex quinquefasciatus or other mosquitoes

    Depositor

    Valentino Gantz

    Article

    Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

    Insert

    pVMG0303_Cq-U6-6_2xBbsI_gRNA-Loop
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    CPIJ039596

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. ptagBFP-TetR-NLS-VP16

    Plasmid

    #103835

    Purpose

    Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arrays

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    tagBFP-TetR-NLS-VP16 (tetR )
    

    Expression

    Mammalian

    Promoter

    SV2

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-C1X-Nup88-mut

    Plasmid

    #87337

    Purpose

    To express human Nup88 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP88 (NUP88 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    shRNA resistant changes

    Promoter

    CMV

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-ChroME(F243Y)-mRuby2-ST

    Plasmid

    #109127

    Purpose

    Modified cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    ChroME(F243Y)-mRuby2-ST
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCNL-C1_RhoB

    Plasmid

    #65697

    Purpose

    Expresses CNL-RhoB in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    RhoB (RHOB Human)
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

19. XE97 mFZ8-CS2+

    Plasmid

    #16800

    Depositor

    Randall Moon

    Article

    Sheldahl et al Curr Biol. 1999 Jul 1;9(13):695-8. doi:

    Insert

    mFZ8-CS2+ (Fzd8 Mouse)
    

    Use

    Xenopus expression

    Available Since

    Sept. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

20. mEmerald-PCNA-19-NLS-4

    Plasmid

    #54222

    Purpose

    Localization: Nucleus, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Lu et al J Cell Biol. 2010 Jun 14;189(6):967-80.

    Insert

    PCNA-19 NLS (PCNA Human)
    

    Tags

    SV40NLS and mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits