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pLHCX-XBP1 mNeonGreen NLS

Plasmid

#115971

Purpose

ER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen (retroviral vector backbone)

Depositor

David Andrews

Article

Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

Insert

X-box binding protein 1 (XBP1 Synthetic, Human)

Use

Retroviral

Tags

HA tag, c-myc NLS, and mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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(559) pAAV EF-1a KRAB-SadCas9 U6-gRNA

Plasmid

#163024

Purpose

Expression of CRISPRi with U6-gRNA (Bsa1 sites)

Depositor

Tonia Rex

Article

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Mammalian codon-optimized SadCas9

Use

AAV

Tags

KRAB, SV40 polyA, and myc NLS

Expression

Mammalian

Mutation

Promoter

hEF-1a

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-Foxo1-WT 6KR

Plasmid

#17560

Depositor

Domenico Accili

Article

Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

Insert

Foxo1 6KR (Foxo1 Mouse)

Use

Tags

Flag and Myc

Expression

Mammalian

Mutation

K242, K245, K259, K262, K271 and K291 were replac…

Promoter

CMV

Available since

Oct. 20, 2008

Availability

Academic Institutions and Nonprofits only
pEF-GalNAc-T2-msGFP2

Plasmid

#140164

Purpose

Expresses the Golgi marker GalnacT2-msGFP2

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

GalNAc-T2-msGFP2 (GALNT2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-Foxo1ADA(pCMV5)

Plasmid

#12149

Depositor

Domenico Accili

Article

Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

Insert

Foxo1 ADA (Foxo1 Mouse)

Use

Tags

Flag and Myc

Expression

Mammalian

Mutation

Threonine 24 to Alanine (A) and Serine 253 to Asp…

Promoter

CMV

Available since

June 13, 2007

Availability

Academic Institutions and Nonprofits only
pZW12 (CK2beta)

Plasmid

#27088

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2beta (CSNK2B Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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FLAG-Foxo1-WT 6KQ

Plasmid

#17562

Depositor

Domenico Accili

Article

Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

Insert

Foxo1 6KQ (Foxo1 Mouse)

Use

Tags

Flag and Myc

Expression

Mammalian

Mutation

K242, K245, K259, K262, K271 and K291 were replac…

Promoter

CMV

Available since

Oct. 20, 2008

Availability

Academic Institutions and Nonprofits only
pMSCV-human-pro-IL-18

Plasmid

#214317

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Retroviral

Tags

Flag and Myc

Expression

Mutation

Promoter

MESV

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pRV.GFP FOXP3

Plasmid

#13250

Depositor

Anjana Rao

Article

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Insert

FOXP3 (FOXP3 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pME-nls-sfGFP

Plasmid

#132973

Purpose

full-length of nuclear localized sfGFP with stop codon in pmE Gateway vector

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Insert

nuclear localized super folder GFP

Use

Gateway vector

Tags

6x myc and SV40 NLS

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pIRES2-EGFP-SHP2WT

Plasmid

#12283

Depositor

Anton Bennett

Article

Kolli et al J Biol Chem. 2004 Jul 9. 279(28):29374-8

Insert

SHP-2WT (PTPN11 Human)

Use

Tags

Myc (not canonical: KLISEEDL)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP-MyosinVa-Ctail

Plasmid

#110169

Purpose

Dominant negative effect by competing with MyosinVa C-terminal tail interactions

Depositor

Jose Esteban

Article

Correia et al Nat Neurosci. 2008 Apr;11(4):457-66. doi

Insert

Myosin Va (Myo5a Mouse)

Use

Tags

EGFP and Myc

Expression

Mammalian

Mutation

amino acids 1444-1853

Promoter

CMV

Available since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pRosa26-GT

Plasmid

#40025

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
beta-globin intron
Neo
tdT-3Myc
diphteria toxin A

Use

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta globin promoter and CMV enhance…

Available since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pEF-ManII-GFP

Plasmid

#160905

Purpose

Expresses the Golgi marker ManII-GFP in mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

MAN2A1 (MAN2A1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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PCXLE-hUL + mTAGBFP2

Plasmid

#74944

Purpose

Dosage control and tracing of reprogramming episomes

Depositor

Ann Zovein

Article

Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

Insert

LMyc/Lin28 (MYCL, LIN28A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3-EMTB-TurboID-V5

Plasmid

#190736

Purpose

Mammalian expression of EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)

Depositor

Jeremy Baskin

Article

Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

Insert

ensconsin (MAP7 Human)

Use

Tags

Myc, TurboID, and V5

Expression

Mammalian

Mutation

Microtubule binding-domain of ensconsin (amino ac…

Promoter

CMV

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-FLEX-synaptoPAC-minWPRE

Plasmid

#153100

Purpose

Cre-conditional expression of synaptoPAC, a fusion of synaptophysin, mScarlet, and bPAC in neurons

Depositor

Dietmar Schmitz

Article

Oldani et al J Neurochem. 2021 Feb;156(3):324-336. do

Inserts

bPAC(WT)
Synaptophysin (Syp Rat)

Use

AAV

Tags

His tag, mScarlet, and myc tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV iGluu

Plasmid

#105832

Purpose

Fast indicator for extracellular glutamate imaging

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR S72T variant

Use

Tags

Myc and PDGFR

Expression

Mammalian

Mutation

Changed Ser 72 to Thr

Promoter

CMV

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-HSF1Δ∼PRD

Plasmid

#71725

Purpose

plasmid expressing mic-his tag phosphorylation-deficient hHSF1

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

phosphorylation-deficient HSF1 (HSF1 Human)

Use

Tags

mac 6xHIS

Expression

Mammalian

Mutation

15 phosphorylation sites in RD mutated to Alanine

Promoter

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP in pDBEcR-pIND(SP1)-DD-W/Bla

Plasmid

#201560

Purpose

Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP-DD (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance.

Depositor

Randy Poon

Article

Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

Insert

DBEcR and EGFP

Use

Unspecified; Sleeping beauty transposon

Tags

DBEcR-myc, FLAG-EGFP, and FLAG-EGFP-DD

Expression

Mammalian

Mutation

Promoter

EF1a and pIND(SP1)

Available since

July 13, 2023

Availability

Academic Institutions and Nonprofits only

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