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We narrowed to 22,403 results for: his

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Showing: 3661 - 3680 of 22403 results

  7. pBAD-bHs

    Plasmid
    #134259
    Purpose
    For L-arabinose inducible bacterial expression of S. typhimurium codon-optimized hyaluronidase (Streptomyces koganeinsis UniProt A0A0U2E2) with N-terminal 6x His tag
    Depositor
    Edwin Manuel
    Article
    Ebelt et al Mol Cancer Ther. 2019 Nov 6. pii: 1535-7
    Insert
    Hyaluronidase from Streptomyces koganeinsis codon-optimized for Salmonella typhimurium
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    codon optimized for Salmonella typhimurium
    Promoter
    AraC
    Available Since
    Nov. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    134259-plasmid-map-sequence-id-264461

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  8. pAAP21

    Plasmid
    #171207
    Purpose
    bacterial expression for HIS purification of the MEG-3 C-terminus with mutations in the HMG-like motif
    Depositor
    Geraldine Seydoux
    Article
    Schmidt et al bioRxiv 2020.10.15.340570
    Insert
    meg-3 (meg-3 Nematode)
    Tags
    8xHIS
    Expression
    Bacterial
    Mutation
    aa545-862, F705A,Y708A,Y713A, N725A
    Available Since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171207-plasmid-map-sequence-id-335796

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  12. GFP-BICD2N

    Plasmid
    #64070
    Purpose
    N-terminal 400 amino acids of mouse BICD2 codon optimized for Sf9 expression with a N-terminal GFP tag and a TEV-cleavable His-ZZ tag for purification (GFP-BICD2N)
    Depositor
    Andrew Carter
    Article
    Schlager et al EMBO J. 2014 Sep 1;33(17):1855-68. doi:
    Insert
    BICD2N (1-400) (Bicd2 Mouse)
    Tags
    GFP and His-ZZ-TCS
    Expression
    Insect
    Mutation
    Codon optimized for Sf9 expression
    Available Since
    Sept. 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    64070-plasmid-map-sequence-id-123551

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  13. pGTM_YR375_SUMO_Protease_001

    Plasmid
    #190063
    Purpose
    Expresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusion
    Depositor
    Gaetano Montelione
    Article
    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi
    Insert
    ULP1_YEAST (ULP1 Budding Yeast)
    Tags
    8X His-MBP (Maltose Binding Protein)
    Expression
    Bacterial
    Mutation
    Construct contains residues 347 – 621
    Promoter
    T7 lac
    Available Since
    Jan. 30, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    190063-plasmid-map-sequence-id-370664

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  14. pEVOL_PylRS(AF)-tRNA

    Plasmid
    #223511
    Purpose
    PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon
    Depositor
    Edward Lemke
    Article
    Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-
    Inserts
    Pyrrolysyl-tRNA Synthetase (AF) (2 copies) (pylS Methanosarcina mazei)
    tRNA Pyl
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Y306A; Y384F
    Promoter
    araBAD, rrnB, and glnS and proK
    Available Since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    223511-plasmid-map-sequence-id-447439

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  17. 7MEQ

    Plasmid
    #176412
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    TMPRSS2 – transmembrane serine protease 2 (TMPRSS2 Human)
    Use
    Baculovirus expresssion
    Tags
    8 x His Tag and Honeybee melittin signal
    Mutation
    etmprss2_S251D/R252D/Q253D/S254D/R255K
    Promoter
    Polyhedrin
    Available Since
    Oct. 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    176412-plasmid-map-sequence-id-345854

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  19. pcDNA3-StagRFP

    Plasmid
    #173014
    Purpose
    Rationally enhanced red fluorescent protein derived from TagRFP-T. For mammalian expression.
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:
    Insert
    StagRFP
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    173014-plasmid-map-sequence-id-343313

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  20. pUD-MSP1D1

    Plasmid
    #161608
    Purpose
    Bacterial expression of mutated membrane scaffold protein ("ultra-dark" MSP1D1) for Nanodisc formation
    Depositor
    Stephen Sligar
    Article
    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do
    Insert
    UD-MSP1D1
    Tags
    7-His tag
    Expression
    Bacterial
    Mutation
    mutant of MSP1D1; all tryptophans and tyrosines w…
    Promoter
    T7
    Available Since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    161608-plasmid-map-sequence-id-317655

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Showing: 3661 - 3680 of 22403 results