We narrowed to 9,187 results for: Pol;

1. sg1+sg2+sg3

   Plasmid

   #113969

   Purpose

   Triple short guide RNA targeting GTATAGCATACATTATACG, TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTC

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   sg1+sg2+sg3
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV Puro SCRIB P305L

   Plasmid

   #88887

   Purpose

   SCRIB - Proline to Lysine mutation at 305 a.a. in MSCV backbone

   Depositor

   Senthil Muthuswamy

   Article

   Zheng et al Nat Cell Biol. 2016 Nov;18(11):1244-1252

   Insert

   SCRIB (SCRIB Human)
   

   Use

   Retroviral

   Mutation

   Proline to Leucine mutation @ 305th amino acid

   Promoter

   MSCV promoter

   Available Since

   March 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. hsp70:Stathmin1-mKate2

   Plasmid

   #105969

   Purpose

   heat shock inducible transgenesis, stathmin stmn1 orig. from addgene #31326

   Depositor

   Caren Norden

   Article

   Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

   Insert

   Stathmin1 (STMN1 Human)
   

   Tags

   mKate2

   Expression

   Bacterial

   Available Since

   Feb. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDECKO-mCherry MALAT1_Enhancer.1

   Plasmid

   #78536

   Purpose

   Experimental plasmid

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6 (for expressing sgRNA) and U6 promoter

   Available Since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLJM1 FRB-myc-RagA

   Plasmid

   #112740

   Purpose

   express FRB-myc-RagA

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   FRB-myc-RagA (RRAGA Human)
   

   Use

   Lentiviral

   Tags

   FRB, myc

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL3-Enhancer-ZC3H12D_PromoterWT

   Plasmid

   #153066

   Purpose

   ZC3H12D promoter region cloned upstream of luciferase reporter gene

   Depositor

   Silvia Monticelli

   Article

   Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

   Insert

   ZC3H12D promoter region (ZC3H12D Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   none

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-Hu_Plk5(WT)

   Plasmid

   #136319

   Purpose

   Mammalian expression of human Plk5-WT with N-terminal EGFP

   Depositor

   Guillermo de Cárcer, Marcos Malumbres

   Article

   de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

   Insert

   PLK5 (PLK5 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXPA-fapO-RgTALsyn

   Plasmid

   #100946

   Purpose

   Expression of Tyrosine Ammonia Lyase from constitutive GAP promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

   Insert

   RgTALsyn
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Silent mutation to remove internal NdeI site on T…

   Promoter

   GAP (Constitutive)

   Available Since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5FRT/TO-Strep/HA-UBE2QL1

   Plasmid

   #124665

   Purpose

   Expresses UBE2QL1 with N-terminal Strep-HA tag in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

   Insert

   UBE2QL1 (UBE2QL1 Human)
   

   Tags

   2xStrep/HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLJM1 FRB-myc-RagC

    Plasmid

    #112741

    Purpose

    express FRB-myc-RagC

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    FRB-myc-RagC (RRAGC Human)
    

    Use

    Lentiviral

    Tags

    FRB, myc

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Frt-hspB10

    Plasmid

    #63101

    Purpose

    Expression of HSPB10 (small HSP) in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb10 (ODF1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-6P1-Ataxin3Q22-C14A

    Plasmid

    #22113

    Depositor

    Henry Paulson

    Article

    Winborn et al J Biol Chem. 2008 Sep 26. 283(39):26436-

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    C14A

    Available Since

    Sept. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pCellFree_G03 FOXM

    Plasmid

    #67118

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    FOXM (FOXM1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pPSP6+t-gfp

    Plasmid

    #48130

    Purpose

    Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-SP6-inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Insert

    gfp
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCellFree_G03 EBNA1BP2

    Plasmid

    #67072

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    EBNA1BP2 EBNA1BP2 EBNA1 binding protein 2 (EBNA1BP2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pDECKO-mCherry MALAT1_Exon.1

    Plasmid

    #78540

    Purpose

    Experimental plasmid

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and U6 (for expressing sgRNA)

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. PLK3 335-646-pEGFP

    Plasmid

    #23267

    Depositor

    Wei Dai

    Article

    Yang et al Cancer Res. 2008 Jun 1. 68(11):4077-85.

    Insert

    PLK3 (PLK3 Human)
    

    Tags

    pEGFP

    Expression

    Mammalian

    Mutation

    aa 335-646

    Available Since

    Dec. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

18. CMV epArcLight

    Plasmid

    #85803

    Purpose

    genetically-encoded fluorescent voltage indicator

    Depositor

    Vincent Pieribone

    Article

    Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

    Insert

    epArcLight
    

    Expression

    Mammalian

    Mutation

    Ci-VSP contains R217Q mutation; ecliptic pHluorin…

    Promoter

    CMV

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5FRT/TO-Strep/HA-UBE2Q2

    Plasmid

    #124668

    Purpose

    Expresses UBE2Q2 with N-terminal Strep-HA tag in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

    Insert

    UBE2Q2 (UBE2Q2 Human)
    

    Tags

    2xStrep/HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-Haunt E1-PuroT2AmCherry-4A KI

    Plasmid

    #92164

    Purpose

    For lncRNA 4_pA(3_SV40 polyA and 1 BGH polyA) homologue recombination mediated knockin

    Depositor

    Xiaohua Shen

    Article

    Yin et al Cell Stem Cell. 2015 May 7;16(5):504-16.

    Insert

    Haunt P-Inr2-cDNA KI homology arms
    

    Use

    Other

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. AAV.CBA.YFP.miR-E_shStk16-1

    Plasmid

    #180391

    Purpose

    Producing AAV that encodes mouse Stk16 shRNA-1 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shStk16-1 (Stk16 Mouse)
    

    Use

    AAV and RNAi

    Promoter

    CBA

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. DsRed +9e

    Plasmid

    #191766

    Purpose

    Expression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9e

    Depositor

    Daniel Gerlich

    Article

    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

    Insert

    DsRed
    

    Tags

    N terminal fusion of positively charged polypetod…

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mOC-STAMP_3xFlag-Myc-CMV26

    Plasmid

    #73577

    Purpose

    Express mouse OC-STAMP in mammalian cells

    Depositor

    Paul Odgren

    Article

    Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

    Insert

    OC-STAMP (Ocstamp Mouse)
    

    Tags

    3x Flag and c-Myc tag

    Expression

    Mammalian

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

24. cARA12-mRFP

    Plasmid

    #181961

    Purpose

    ARA12 protein tagged with mRFP, under control of TBA2 promoter

    Depositor

    George Haughn

    Article

    Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

    Insert

    ARA12 (ARA12 Mustard Weed)
    

    Tags

    mRFP

    Expression

    Plant

    Promoter

    TBA2

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMH-HT_SsCHAD-H29A-R32A-R36A-R69A

    Plasmid

    #127787

    Purpose

    Expresses a SsCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    SsCHAD (SULM_RS11045 Sulfolobus solfataricus)
    

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    mutations in His29, Arg32, Arg36 and Arg69 to Ala

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pIS1 DICER1 long-mut-let-7

    Plasmid

    #21651

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    Dicer 1 (DICER1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutant let-7 microRNA binding sites

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEMS2117

    Plasmid

    #49142

    Purpose

    AAV plasmid with smCBA promoter driving expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-smCBA-iCre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    smCBA

    Available Since

    May 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. LLP250 pEF1a-TALE-SunTag

    Plasmid

    #100939

    Purpose

    Empty TALE vector fused to SunTagx10 driven by EF1a promoter, with 3xHA tag, no PURO gene

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    TALE insertion site, SunTag array (10xGCN4)
    

    Use

    TALEN

    Tags

    3xHA

    Expression

    Mammalian

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. pCDF11-SFPQ(214-598)

    Plasmid

    #135440

    Purpose

    Expresses SFPQ 214-598 with TEV-cleavable hexahistidine tag

    Depositor

    Charles Bond

    Article

    Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

    Insert

    splicing factor proline and glutamine rich (SFPQ Human)
    

    Tags

    Hexahistidine

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pIS1 DICER1 long-mut-all

    Plasmid

    #21653

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    Dicer 1 (DICER1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutated let-7 and miR103/107 microRNA binding sit…

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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