Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 116,150 results for: mpi

Showing: 9201 - 9250 of 116150 results

  1. LLhRafR-pHG165a 3mut

    Plasmid
    #220274
    Purpose
    Constitutive expression of a chimeric LacI:RafR transcription repressor
    Depositor
    Liskin Swint-Kruse
    Insert
    LLhRafR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    V52N/Q55L/Q62H
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    220274-plasmid-map-sequence-id-436468

    Enlarge

  2. LLhSalR-pHG165a 3mut

    Plasmid
    #220276
    Purpose
    Constitutive expression of a chimeric LacI:SalR transcription repressor
    Depositor
    Liskin Swint-Kruse
    Insert
    LLhSalR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    V52L/Q55G/Y62R
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    220276-plasmid-map-sequence-id-436470

    Enlarge

  3. LLhScrR-pHG165a 3mut

    Plasmid
    #220278
    Purpose
    Constitutive expression of a chimeric LacI:ScrR transcription repressor
    Depositor
    Liskin Swint-Kruse
    Insert
    LLhScrR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    V52I/Q62S/G120S
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    220278-plasmid-map-sequence-id-436472

    Enlarge

  4. pML107-NET1

    Plasmid
    #232893
    Purpose
    Plasmid expressing Cas9 and gRNA GTGCCACTAATGGATCCATG which targets the NET1 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    NET1 gRNA (NET1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    232893-plasmid-map-sequence-id-470393

    Enlarge

  5. pML107-VRG4

    Plasmid
    #232899
    Purpose
    Plasmid expressing Cas9 and gRNA TCGCATAGCCCAGTATACGT which targets the VRG4 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    VRG4 gRNA (VRG4 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    232899-plasmid-map-sequence-id-471178

    Enlarge

  6. pML107-SAM50

    Plasmid
    #232895
    Purpose
    Plasmid expressing Cas9 and gRNA AATAGTTTATGTAAGAACAG which targets the SAM50 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    SAM50 gRNA (SAM50 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    232895-plasmid-map-sequence-id-470389

    Enlarge

  7. SARS2_HisSUMOTEV-nsp7/nsp8

    Plasmid
    #234405
    Purpose
    Bacterial expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Inserts
    Nsp7_SARS2
    Nsp8_SARS2
    Use
    Tags
    His6-SUMO-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    234405-plasmid-map-sequence-id-469465

    Enlarge

  8. NSP12:4393-5324_Nbio_Chis_BV

    Plasmid
    #234377
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    Nsp12_SARS2
    Use
    Baculovirus expresssion
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    234377-plasmid-map-sequence-id-469461

    Enlarge

  9. NSP13:5325-5925_BVnAvi

    Plasmid
    #234375
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    Nsp13_SARS2
    Use
    Baculovirus expresssion
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    234375-plasmid-map-sequence-id-469460

    Enlarge

  10. SARS1_NSP13_BV_NBIO-TEV_CTHROM-his

    Plasmid
    #234382
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    Nsp13_SARS
    Use
    Baculovirus expresssion
    Tags
    LVPRGSGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    234382-plasmid-map-sequence-id-469458

    Enlarge

  15. EP003: CAG::GEM7-HaloTag

    Plasmid
    #209303
    Purpose
    Expression plasmid for GEM7 (encapsulin from R. rubrum) with C-terminal HaloTag suitable for expression in mice via in utero electroporation
    Depositor
    Adam Cohen
    Insert
    GEM7
    Use
    AAV
    Tags
    HaloTag
    Expression
    Mutation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    209303-plasmid-map-sequence-id-414428

    Enlarge

  16. EP004: pAAV TRE::GEM7-HaloTag

    Plasmid
    #209304
    Purpose
    Doxycycline-gated expression plasmid (TetOFF) for GEM7 (encapsulin from R. rubrum) with C-terminal HaloTag suitable for expression in mice
    Depositor
    Adam Cohen
    Insert
    GEM7
    Use
    AAV
    Tags
    HaloTag
    Expression
    Mutation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    209304-plasmid-map-sequence-id-414377

    Enlarge

  19. TRE-FRT-MCS-WPRE-SV40 - GCH27

    Plasmid
    #230828
    Purpose
    AAV cloning vector for Flpo-dependent expression under the TRE promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector, flp/frt
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    230828-plasmid-map-sequence-id-466645

    Enlarge

  20. pAN68

    Plasmid
    #164525
    Purpose
    Retroviral expression of CENP-R uORF-1
    Depositor
    Iain Cheeseman
    Insert
    CENP-R uORF-1 (ITGB3BP Human)
    Use
    Retroviral
    Tags
    -6xhis-precission-GFP
    Expression
    Mutation
    Promoter
    Available since
    March 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    164525-plasmid-map-sequence-id-328004

    Enlarge

  21. pAG Retro EGFP Neo

    Plasmid
    #234464
    Purpose
    Control retroviral vector expressing Green Fluorescent Protein. Neomycin selection.
    Depositor
    Addgene Research Program, Melina Fan
    Insert
    Green Fluorescent Protein
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 21, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234464-plasmid-map-sequence-id-464634

    Enlarge

  22. pCAG/TSF-VPS34

    Plasmid
    #232916
    Purpose
    Mammalian Expression
    Depositor
    James Hurley
    Insert
    VPS34 (PIK3C3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    232916-plasmid-map-sequence-id-465438

    Enlarge

  23. pCAG/VPS15(R36D)-TSF

    Plasmid
    #232913
    Purpose
    Mammalian Expression
    Depositor
    James Hurley
    Insert
    VPS15 (PIK3R4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R36D
    Promoter
    Available since
    March 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    232913-plasmid-map-sequence-id-465433

    Enlarge

  24. pCS2-zH2B-GFP

    Plasmid
    #226561
    Purpose
    Express H2B-GFP in zebrafish.
    Depositor
    Yong Zhang
    Insert
    h2b (h2bc1 Zebrafish)
    Use
    Synthetic Biology; Zebrafish expression
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    Available since
    March 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    226561-plasmid-map-sequence-id-461800

    Enlarge

  25. pGL3_DDX25

    Plasmid
    #232359
    Purpose
    Enhancer near DDX25, chr11:125935511-125936012 cloned into pGL3
    Depositor
    Joanna Wysocka
    Insert
    DDX25 (DDX25 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232359-plasmid-map-sequence-id-463060

    Enlarge

  26. pGL3_MSR1_rightneg0topos1

    Plasmid
    #232389
    Purpose
    MSR1 enhancer, right buffering Coordinator to sensitizing Coordinator
    Depositor
    Joanna Wysocka
    Insert
    MSR1 (MSR1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    MSR1 enhancer, right buffering Coordinator to sen…
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232389-plasmid-map-sequence-id-462852

    Enlarge

  27. pML104-HygMx4-URA3-3

    Plasmid
    #232886
    Purpose
    Plasmid expressing Cas9 and gRNA GAAGTAACAAAGGAACCTAG which targets the URA3 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    URA3 gRNA (URA3 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232886-plasmid-map-sequence-id-462214

    Enlarge

  28. pML104-HygMx4-URA3-2

    Plasmid
    #232885
    Purpose
    Plasmid expressing Cas9 and gRNA TCGTACCACCAAGGAATTAC which targets the URA3 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    URA3 gRNA (URA3 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232885-plasmid-map-sequence-id-462211

    Enlarge

  29. MigR1 GFP Mouse PHGDH

    Plasmid
    #220591
    Purpose
    Retroviral mammalian expression vector with GFP for overexpression of mouse PHGDH
    Depositor
    Russell Jones
    Insert
    Ms PHGDH (Phgdh Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    220591-plasmid-map-sequence-id-436554

    Enlarge

  30. pML104-HIS3-3

    Plasmid
    #232882
    Purpose
    Plasmid expressing Cas9 and gRNA AAACCTTTTTACTCCACGCA which targets the HIS3 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    HIS3 gRNA (HIS3 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232882-plasmid-map-sequence-id-462144

    Enlarge

  31. pML107-ZIM17

    Plasmid
    #232901
    Purpose
    Plasmid expressing Cas9 and gRNA AAATGTCTCACTTTGCAGTG which targets the ZIM17 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    ZIM17 gRNA (ZIM17 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232901-plasmid-map-sequence-id-462145

    Enlarge

  32. pML104-HIS3-2

    Plasmid
    #232881
    Purpose
    Plasmid expressing Cas9 and gRNA CCAAGTTCGACAACTGCGTA which targets the HIS3 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    HIS3 gRNA (HIS3 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232881-plasmid-map-sequence-id-462138

    Enlarge

  33. pML107-ADE13

    Plasmid
    #232887
    Purpose
    Plasmid expressing Cas9 and gRNA GTAGCAGCAAAAGAAGACAA which targets the ADE13 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    ADE13 gRNA (ADE13 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232887-plasmid-map-sequence-id-462137

    Enlarge

  34. pML107-LAS17

    Plasmid
    #232892
    Purpose
    Plasmid expressing Cas9 and gRNA AATACCCTGAATTCTGCCGG which targets the LAS17 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    LAS17 gRNA (LAS17 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232892-plasmid-map-sequence-id-462139

    Enlarge

  35. pRS313-rad53-5004

    Plasmid
    #232902
    Purpose
    Plasmid carrying the rad53-5004 allele with its native promoter and terminator.
    Depositor
    Jolanda Van Leeuwen
    Insert
    RAD53 (RAD53 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    T600C, A680G, A1094T, C1615T, C2228A
    Promoter
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232902-plasmid-map-sequence-id-462087

    Enlarge

  36. 3'dmDR-CMV-CasRx-bGHpolyA

    Plasmid
    #229556
    Purpose
    Expresses CasRx (RfxCas13d) with double processed direct repeats (15nt) and a non-target spacer in between at 3' end
    Depositor
    Omar Akbari
    Insert
    CasRx
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    229556-plasmid-map-sequence-id-455012

    Enlarge

  37. 1203U-U6-gRNA-pCMV-wtCas9-NG-P2A-mcherry-3'dmDR

    Plasmid
    #221448
    Purpose
    Expresses wtCas9-NG with double processed direct repeats (15nt) and a non-target spacer in between at 3' end, mCherry tag, and the gRNA targeting HEK293T-site3
    Depositor
    Omar Akbari
    Inserts
    wtCas9-NG
    mCherry
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221448-plasmid-map-sequence-id-437819

    Enlarge

  38. pMX-Flag-GFP_EDIL3

    Plasmid
    #227957
    Purpose
    Co-localisation experiment
    Depositor
    Alessandro Ori
    Insert
    EDIL3 (EDIL3 Human)
    Use
    Lentiviral
    Tags
    3xFLAG-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    227957-plasmid-map-sequence-id-461332

    Enlarge

  39. AAV-hSyn-PiGM-Iq(535/81, eGFP)

    Plasmid
    #221612
    Purpose
    AAV encoding PiGM-Iq with minimal RGS10 domain, CRY2 (535 amino acid version), CIB1 (81 amino acid version).
    Depositor
    John Lin
    Insert
    PiGM-Iq (535/81,eGFP)
    Use
    AAV
    Tags
    Expression
    Mutation
    RGS2 1-53 truncation
    Promoter
    Human Synapsin
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    221612-plasmid-map-sequence-id-461237

    Enlarge

  40. pCCG_nit9fl_CNX1_linker

    Plasmid
    #228414
    Purpose
    Expression of NIT-9 with CNX1 linkage region in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9 (AT5G61790 Mustard Weed)
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    removal of the linkage region and insertion of th…
    Promoter
    Available since
    Jan. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    228414-plasmid-map-sequence-id-459560

    Enlarge

  42. pBS-ACTB-C200-T7

    Plasmid
    #229850
    Purpose
    Donor vector for the ACTB site containing T7 sequence for detection of HR products.
    Depositor
    Natsuko Chiba
    Insert
    Partial sequence of ACTB gene
    Use
    Hr donor vector for human actb gene.
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    229850-plasmid-map-sequence-id-457516

    Enlarge

  43. pCG-scr

    Plasmid
    #229847
    Purpose
    Expresses wild-type Cas9 and scrambled gRNA.
    Depositor
    Natsuko Chiba
    Insert
    guide RNA with scrambled sequence
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    229847-plasmid-map-sequence-id-457515

    Enlarge

  44. pDual_dsCas9_Venus_hs_NC_chr1

    Plasmid
    #214682
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214682-plasmid-map-sequence-id-428816

    Enlarge

  45. pDual_dsCas9_Venus_hs_NC_chr2

    Plasmid
    #214683
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human non-coding DNA region of chromosome 2 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214683-plasmid-map-sequence-id-428804

    Enlarge

  46. pDual_dsCas9_Venus_hs_TP73

    Plasmid
    #214685
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human TP73
    Depositor
    Kevin Janes
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214685-plasmid-map-sequence-id-428818

    Enlarge

  47. pDual_dsCas9_BFP_hs_NC_chr1

    Plasmid
    #214686
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214686-plasmid-map-sequence-id-428810

    Enlarge

  48. pDual_dsCas9_BFP_hs_TP73

    Plasmid
    #214687
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human TP73
    Depositor
    Kevin Janes
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214687-plasmid-map-sequence-id-428806

    Enlarge

Showing: 9201 - 9250 of 116150 results