We narrowed to 11,278 results for: VARS

1. p2T-CAG-MCS-P2A-GFP-PuroR

   Plasmid

   #107186

   Purpose

   Base plasmid for cloning gene duplication alleles with eGFP reporter.

   Depositor

   Richard Sherwood

   Article

   Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

   Type

   Empty backbone

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. LentiU6-hMYOD1 gRNA_1-MS2-Puro

   Plasmid

   #192673

   Purpose

   Lentiviral expression of sgRNA targeting hMYOD1 promoter to activate human MYOD1 transcription

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   Human MYOD1 activating gRNA #1 (MYOD1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVG1

   Plasmid

   #111444

   Purpose

   Unified Solo vector pV1382 + sgScADE2 + ScADE2 stop codon repair tempate

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgScADE2/stop-codon repair
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFRT-PonA-BI-Gl-GFP-PTC-mCherry-WT (5FP-WP)

   Plasmid

   #189795

   Purpose

   Express GFP/mCherry linked wild-type and PTC-containing beta-globin bi-directionally under ponasteron A inducible promoter in mammalian cells

   Depositor

   Robert Singer

   Article

   Sato et al Nat Commun. 2021 Dec 10;12(1):7203. doi:

   Insert

   beta-globin (HBB Mouse, Human)
   

   Expression

   Mammalian

   Promoter

   Ponasterone A inducible

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEThT-CAHS1

   Plasmid

   #90027

   Purpose

   To express His-tagged CAHS1 in bacteria

   Depositor

   Takekazu Kunieda

   Article

   Yamaguchi et al PLoS One. 2012;7(8):e44209.

   Insert

   CAHS1
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLQ1371-sgGAL4

   Plasmid

   #121514

   Purpose

   sgGAL4 control. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

   Depositor

   Ovijit Chaudhuri, Stanley Qi

   Article

   Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

   Insert

   sgGAL4
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   April 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMDJ12 - pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]

   Plasmid

   #159799

   Purpose

   Expression construct with ubiquitous expression to test fluorescence level on microscope

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac1-Ac-AChBP

   Plasmid

   #63128

   Purpose

   Expression of Aplysia californica AChBP

   Depositor

   Titia Sixma

   Article

   Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

   Insert

   AChBP
   

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pDUP1

   Plasmid

   #223516

   Purpose

   inactive cogfp under control of Ptet, cogfp under control of Ptac

   Depositor

   Yolanda Schaerli

   Article

   Ljiljana et al eLife13:RP97216

   Inserts

   CoGFP
   CoGFP inactivated
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   anti-dimerization mutations S129G, 22 C154S, D156…

   Promoter

   Ptac, Ptac and Ptet, and Ptet

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEY51

    Plasmid

    #191054

    Purpose

    mec-3 fluorescent neural reporter driving nuclear CyOFP1 and mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    mec-3 (mec-3 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEY52

    Plasmid

    #191055

    Purpose

    odr-1 fluorescent neural reporter driving nuclear mNeptune2.5 and mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    odr-1 (odr-1 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFRT-PonA-BI-Gl-GFP-WT-mCherry-WT (5FP-WW)

    Plasmid

    #189794

    Purpose

    Express GFP/mCherry linked wild-type beta-globin bi-directionally under ponasteron A inducible promoter in mammalian cells

    Depositor

    Robert Singer

    Article

    Sato et al Nat Commun. 2021 Dec 10;12(1):7203. doi:

    Insert

    beta-globin (HBB Mouse, Human)
    

    Expression

    Mammalian

    Promoter

    Ponasterone A inducible

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti CRISPR V2 sgMTHFD1L_1

    Plasmid

    #106316

    Purpose

    Express Cas9 and sgRNA targeting MTHFD1L

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting MTHFD1L
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSws1-nfsB-CFP

    Plasmid

    #86375

    Purpose

    for zebrafish transgenesis, expression of nfsB-CFP, also expresses EGFP

    Depositor

    Rachel Wong

    Article

    Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

    Insert

    nitroreductase (nfsB bacteria)
    

    Use

    Zebrafish

    Tags

    ECFP

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAB088h

    Plasmid

    #79215

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1+2) gIII, luxAB; Ppro1 434cI-TnCAD-F3
    

    Expression

    Bacterial

    Promoter

    PlacZ-opt (OR1+2); Ppro1

    Available Since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3_attR1-ORF-attR2-NTEV-TCS-GV-2xHA_DEST

    Plasmid

    #194385

    Purpose

    Gateway Destination vector for split TEV assays

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    attR1-ORF-attR2-NTEV-TCS-GV-2xHA
    

    Tags

    2xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEThT-SAHS2

    Plasmid

    #90026

    Purpose

    To express His-tagged SAHS2 in bacteria

    Depositor

    Takekazu Kunieda

    Article

    Yamaguchi et al PLoS One. 2012;7(8):e44209.

    Insert

    SAHS2
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-FLAG-SpCas9-HF1

    Plasmid

    #126770

    Purpose

    Expression of increased fidelity SpCas9-HF1 in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    SpCas9-HF1
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    T7

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS2+ macroH2A1.2-GFP-HA

    Plasmid

    #30515

    Depositor

    John Gurdon

    Article

    Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

    Insert

    macroH2A1.2-C (Macroh2a1 Mouse)
    

    Use

    Xenopus/zebrafish expression.

    Tags

    eGFP and triple HA

    Expression

    Mammalian

    Mutation

    NO STOP

    Available Since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. CMV+ plasmid

    Plasmid

    #169740

    Purpose

    Unregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels. This plasmid has additional sequences in the UTRs to increase gene expression levels.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    pCMV-tetO2

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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