We narrowed to 4,941 results for: puc

1. pAT9675-CBE

   Plasmid

   #163007

   Purpose

   plasmid expressing CBE

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   CBE
   

   Use

   CRISPR

   Tags

   3xFLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMTMVi-M

   Plasmid

   #118757

   Purpose

   Intermediate plasmid to build tobacco mosaic virus recombinant clones that move systemically throughout the plant.

   Depositor

   Jose-Antonio Daros

   Article

   Shi et al Plant Biotechnol J. 2018 Nov 22. doi: 10

   Insert

   Tobacco mosaic virus cDNA fragment with a cloning polylinker
   

   Use

   Intermediate to build tobacco mosaic virus recomb…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAT9748-dCBE

   Plasmid

   #163008

   Purpose

   plasmid expressing CBE with dCas9

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   dCBE
   

   Use

   CRISPR

   Tags

   3xFLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. TC1316

   Plasmid

   #164883

   Purpose

   CMV-bpNLS-dCas13d-bpNLS-ADARdd-mCherry, ADARdd embeded in loop3

   Depositor

   Tian Chi

   Article

   Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:

   Insert

   dcas13d-ADARdd
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   dCas13d L560-G586 deleted

   Promoter

   CMV, T7

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pILGFPI7

   Plasmid

   #83555

   Purpose

   Used to evaluate the expression output of CUP1 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   CUP1 promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   CUP1

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. AICSDP-72:TFAM-mEGFP

   Plasmid

   #159745

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human TFAM

   Depositor

   Allen Institute for Cell Science

   Insert

   TFAM Homology Arms with linker-mEGFP (TFAM Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   Oct. 13, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pL0-BvDODAα1

   Plasmid

   #162528

   Purpose

   Beta vulgaris 4,5-DOPA dioxygenase alpha 1 coding sequence in pICH41308 MoClo Golden Gate level 0 acceptor for CDS1 modules.

   Depositor

   Sam Brockington

   Article

   Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

   Insert

   DODAα1
   

   Use

   Puc19-derived

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDAS12230_pegRNA-PEAR-GFP(10PBS-24RT)-mCherry

   Plasmid

   #177182

   Purpose

   plasmid expressing a pegRNA targeting the PEAR-GFP plasmid along with an mCherry marker

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Insert

   pegRNA targeting the PEAR-GFP plasmid
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pILGFP91

   Plasmid

   #83548

   Purpose

   Used to evaluate the expression output of PGK1 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   PGK1 promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PGK1

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAVS1_Puro_hPGK1_mScarlet-I-TOSI_Donor

    Plasmid

    #178087

    Purpose

    HDR donor plasmid to introduce the mScarlet-I mTOR signaling indicator (TOSI) cassette to AAVS1 using AAVS1 T2 sgRNA.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    mScarlet-I mTOR signaling indicator (TOSI)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human PGK1

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTO149_RFP_CauNEO

    Plasmid

    #177277

    Purpose

    Encodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.

    Depositor

    Teresa O'Meara

    Article

    Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

    Insert

    RFP, CauNEO
    

    Use

    Tags

    ENO1-RFP

    Expression

    Bacterial

    Mutation

    All CUG Leucine codons were replaced with the syn…

    Promoter

    AgTEF2

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDGE476

    Plasmid

    #153267

    Purpose

    M2E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDD315 (mTurquoise2^SEC^2xHA)

    Plasmid

    #73343

    Purpose

    mTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Zhang et al J Cell Biol. 2023 Sep 4;222(9):e20230210

    Insert

    mTurquoise2-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    2xHA and C. elegans codon-optimized mTurquoise2

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCE38

    Plasmid

    #174432

    Purpose

    C. auris LEUpOUT HYG marker CAS9 expression construct. Use with pCE41 gRNA expression construct.

    Depositor

    Clarissa Nobile

    Article

    Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

    Insert

    C. auris LEU2 1 of 2, pENO1, Cas9, HYG 1 of 2
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCE41

    Plasmid

    #174433

    Purpose

    C. auris LEUpOUT HYG marker gRNA expression construct. Use with pCE38 CAS9 expression construct.

    Depositor

    Clarissa Nobile

    Article

    Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

    Insert

    HYG 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMGS54 (AID-P2A-Hygromycin)

    Plasmid

    #126583

    Purpose

    An AID-P2A-HygR cassette to tag the protein of interests with auxin-inducible degron at the c-terminal

    Depositor

    Michael Guertin

    Article

    Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

    Insert

    AID-P2A-HygR
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDAS12137_sgRNA-PEAR-GFP-nick(+17)-mCherry

    Plasmid

    #177183

    Purpose

    plasmid expressing an sgRNA targeting the PEAR-GFP plasmid along with an mCherry marker

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    sgRNA targeting the PEAR-GFP plasmid
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. qTAG-AAVS1-Ef1a-Zeo

    Plasmid

    #227268

    Purpose

    Empty AAVS1 targeting donor for the insertion of Zeo and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPAP_BGL1

    Plasmid

    #183735

    Purpose

    Expresses Aspergillus aculeatus β-glucosidase (BGL1)

    Depositor

    Jun Ishii

    Article

    Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

    Insert

    β-glucosidase (AaBGL1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1 promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEF1a-donor_opt(R2Tg)-GFP(intron)

    Plasmid

    #223252

    Purpose

    Mammalian expression of the optimized R2Tg RNA donor

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg donor_opt
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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