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LITE1.0_pAAV_hSyn_CRY2PHR-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

Plasmid

#47454

Purpose

epiLITE / LITE1.0 CRY2PHR fused to SID4X transcriptional repressor effector domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

CRY2PHR-NLS-SID4X

Use

AAV

Tags

2A_phiLOV2.1

Expression

Mammalian

Promoter

hSyn human synapsin (mammalian neuronal expressio…

Available Since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only

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dmBACCS2VL-IRES-dOrai

Plasmid

#67629

Purpose

Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOrai

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2VL-IRES-dOrai

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2-ER-EmEGFP

Plasmid

#169000

Purpose

Express ER-EmEGFP in zebrafish

Depositor

Noboru Mizushima

Article

Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

Insert

ER-EGFP (encoding the signal sequence of human calreticulin mRNA and EGFP-KDEL) (CALR Synthetic, Human)

Use

Unspecified

Tags

EGFP

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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CRY2-mCherry-iTrkA

Plasmid

#106168

Purpose

mammalian expression of CRY2PHR (aa 1-498) fused to mCherry and intracellular domain of TrkA (aa 450-799)

Depositor

Bianxiao Cui

Article

Duan et al ACS Synth Biol. 2018 Jul 20;7(7):1685-16

Insert

CRY2PHR mCherry iTrkA (CRY2 Rat, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO 3xFlag-m-PKD1 S4134D-2xMyc/His

Plasmid

#83453

Purpose

Full-length murine PC1 with a Calmodulin binding mutation for Eukaryotic Expression (1037)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

polycystin-1 (Pkd1 Mouse)

Tags

2xMyc, 3xFlag, and 6xHis

Expression

Mammalian

Mutation

S4144D

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaax

Plasmid

#173875

Purpose

Mammalian expression of mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaax (opto-Ral)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mVenus-NES-SspB-RGL2GEF-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-NES-SspB-RGL2-GEF-P2A-iLIDcaax

Plasmid

#173876

Purpose

Mammalian expression of mCherry-NES-SspB-RGL2-GEF-P2A-iLIDcaax (opto-Ral)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mCherry-NES-SspB-RGL2GEF-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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psicheck-2-Casp8

Plasmid

#48172

Purpose

contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of CASP8 (CASP8 Human)

Use

Luciferase

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
pDisplay-GRAB_OTmut

Plasmid

#185385

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in mammalian cells

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

GPCR activation based oxytocin control sensor GRAB_OTmut

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-nirButterfly

Plasmid

#136591

Purpose

AAV plasmid for CaMKII-driven expression of a genetically-encoded voltage indicator nirButterfly

Depositor

Vladislav Verkhusha

Article

Monakhov et al ACS Chem Neurosci. 2020 Nov 4;11(21):352

Insert

nirButterfly

Use

AAV

Expression

Mammalian

Promoter

CaMKIIa

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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Twitch-3 pRSETB

Plasmid

#48204

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-3A

Tags

6xHIS, cpVenusCD, and mCerulean3

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only
mEmerald-MyosinIIC-C-18

Plasmid

#54194

Purpose

Localization: Cytoskeleton, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Ebrahim et al Curr Biol. 2013 Apr 22;23(8):731-6. doi:

Insert

MyosinIIC

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pAAV-hRREB1-RFP

Plasmid

#22924

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

human ras responsive element binding protein 1 driving DsRedExpress (RREB1 Human)

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-4493NES

Plasmid

#175811

Purpose

The lentiviral vector for Booster-PKA, a Red-Shifted genetically encoded FRET biosensor for monitoring PKA activity.

Depositor

Michiyuki Matsuda

Article

Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

Insert

Booster-PKA

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO CD16.7-h-PKD1 FLM R4114A

Plasmid

#83461

Purpose

Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (503)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

Amino-acids 4078-4303, R4113A

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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CKAP2-mNeonGreen pHAT

Plasmid

#175947

Purpose

Bacterial expression of mmCKAP2-mNeonGreen, N-terminal 6xHis-tag, C-terminal Strep-tagII

Depositor

Susanne Bechstedt

Article

McAlear et al Elife. 2022 Jan 14;11. pii: 72202. doi:

Insert

CKAP2 (Ckap2 Mouse)

Tags

6His, StrepTagII, and mNeonGreen

Expression

Bacterial

Promoter

T7

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBstSK+IRES-V5-IPpoI (mutant)

Plasmid

#206322

Purpose

A plasmid that facilitates production of mRNA encoding V5 epitope tagged catalytically dead I-PpoI

Depositor

Brian McStay

Article

van Sluis et al Genes Dev. 2015 Jun 1;29(11):1151-63. do

Insert

IRES element-V5 epitope tag-mutated IPpoI open reading frame

Use

Mrna production

Tags

V5-IPpoI fusion protein (mutant version)

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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CMV epArcLight

Plasmid

#85803

Purpose

genetically-encoded fluorescent voltage indicator

Depositor

Vincent Pieribone

Article

Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

Insert

epArcLight

Expression

Mammalian

Mutation

Ci-VSP contains R217Q mutation; ecliptic pHluorin…

Promoter

CMV

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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tdminiSOG-H2B-C-10

Plasmid

#57804

Purpose

Localization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

H2B (H2BC11 Human)

Tags

tdminiSOG

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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miniSOG-Tubulin-6

Plasmid

#57777

Purpose

Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Tubulin (TUBA1B Human)

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits