We narrowed to 7,791 results for: amph

1. pKm-HGBE

   Plasmid

   #170334

   Purpose

   a modularized single-plasmid glycosylase base editing (C-to-G) tool for Sinorhizobium meliloti

   Depositor

   Deqiang Duanmu

   Article

   Wang et al Front Microbiol. 2021 Jun 18;12:686008.

   Insert

   Rhizobium codon-optimized GBEs
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Cas9n-D10A

   Promoter

   Available since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJH773_pTech_PlldR-HHRz-supP(opal)

   Plasmid

   #163732

   Purpose

   Lactate-inducible expression of supP(opal), preceded by a hammerhead ribozyme

   Depositor

   Matthew Bennett

   Article

   Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

   Insert

   hhrz-supP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. LbCas12a-gRNA_BbsI_CAM

   Plasmid

   #183222

   Purpose

   LbCas12a gRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   LbCas12a guide RNA containing BbsI sites in spacer sequence
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLG015 (RADAR)

   Plasmid

   #157893

   Purpose

   Expresses the RADAR defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   RADAR subtype containing auxillary gene rdrD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLG016 (ApeA)

   Plasmid

   #157894

   Purpose

   Expresses the ApeA defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   ApeA (putative HEPN RNase)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKM197

   Plasmid

   #132665

   Purpose

   pyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.

   Depositor

   Joseph Sorg

   Article

   Bhattacharjee et al mSphere. 2020 Mar 4;5(2):e00941-19. doi:

   Insert

   Upstream & Downstream pyrE deletion region
   

   Use

   E. coli - c. difficile shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKm-HABE

   Plasmid

   #170332

   Purpose

   a modularized single-plasmid adenosine base editing (A-to-G) tool for Sinorhizobium meliloti

   Depositor

   Deqiang Duanmu

   Article

   Wang et al Front Microbiol. 2021 Jun 18;12:686008.

   Insert

   Rhizobium codon-optimized ABEs
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Cas9n-D10A

   Promoter

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST-APLO

   Plasmid

   #112805

   Purpose

   gateway cloning destination vector to express LexAop2 driven transgene in Drosophila

   Depositor

   Chun Han

   Article

   Poe et al Proc Natl Acad Sci U S A. 2017 Sep 19;11

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSJ111

   Plasmid

   #78707

   Purpose

   Modular vector for constitutive secreted amylase reporter expression

   Depositor

   Wiep Klaas Smits

   Article

   Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

   Insert

   amyEopt
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pveg

   Available since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR1K18ms

    Plasmid

    #72644

    Purpose

    Gateway-compatible sucrose counter-selection allelic exchange vector for Pseudomonas syringae

    Depositor

    Brian Kvitko

    Article

    Mijatovic et al Phytopathology. 2021 Dec;111(12):2176-21

    Type

    Empty backbone

    Use

    Sucrose counter-selection allelic exchange vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. MTK2_005

    Plasmid

    #123700

    Purpose

    Encodes pCAG as a Type 2 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pCAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST-CMV-N-Tandem-mCherry-EGFP

    Plasmid

    #123216

    Purpose

    N-terminal Tandem mCherry EGFP tag. Gateway destination vector for mammalian expression.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLPS-hSox2-gfp

    Plasmid

    #49390

    Purpose

    mammalian expression of human Sox2 fused to EGFP

    Depositor

    Randall McKinnon

    Insert

    Sox2 (SOX2 Human)
    

    Use

    Cre/Lox; Acceptor vector

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    TGA (stop) to CCA (Pro) at nt 1027 with respect t…

    Promoter

    CMV

    Available since

    Nov. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAFi-dHdrA

    Plasmid

    #184907

    Purpose

    carrying CRISPR/dCas9 system for gene knockdown in Acidithiobacillus ferridurans

    Depositor

    Radhakrishnan Mahadevan

    Article

    Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

    Insert

    Cm-sgRNA-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. MTK3_036

    Plasmid

    #123750

    Purpose

    Encodes mScarlet as a Type 3 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    mScarlet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. MTK4_003

    Plasmid

    #123812

    Purpose

    Encodes rabbit beta-globin polyadenylation signal as a Type 4 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    rglb pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLys-M1

    Plasmid

    #109382

    Purpose

    Arabinose inducible VioB-mCherry expression (stronger RBS). Used to test burden response in E. coli

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pARABAD-VioB-mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLCS104

    Plasmid

    #209357

    Purpose

    Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal HaloTag fusion protein.

    Depositor

    Lucia Strader

    Article

    Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDRFLIP38

    Plasmid

    #65516

    Purpose

    FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-edCit-attR1 and attR2-edCer-cMyc

    Expression

    Yeast

    Mutation

    Promoter

    pPMA1

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-sgRNA-Lib

    Plasmid

    #53121

    Purpose

    sgRNA expression construction in a 3rd generation lentiviral backbone

    Depositor

    Wensheng Wei

    Article

    Zhou et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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