4,180 results

1. pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)

   Plasmid

   #71719

   Purpose

   Expressing a GFP-tagged degron in the soma of C. elegans

   Depositor

   Abby Dernburg

   Article

   Zhang et al Development. 2015 Nov 9. pii: dev.129635

   Insert

   auxin-responsive protein IAA17 (AXR3 Mustard Weed)
   

   Tags

   EmGFP

   Expression

   Worm

   Mutation

   minimal degron sequence (71-114aa) with start cod…

   Promoter

   eef-1A.1 (eft-3)

   Available Since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. paavCAG-Jx-rev-post-mGRASP-2A-dTomato

   Plasmid

   #34913

   Purpose

   JxON-post

   Depositor

   Jinhyun Kim

   Article

   Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

   Insert

   postsynaptic mGRASP
   

   Use

   AAV and Cre/Lox

   Promoter

   CAG

   Available Since

   Nov. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-Foxo4

   Plasmid

   #17549

   Depositor

   Domenico Accili

   Insert

   FoxO4 (FOXO4 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Oct. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-mNeptune2-N

   Plasmid

   #41645

   Purpose

   Expresses far-red FP mNeptune2 fused to Dronpa145N. Dronpa145N oligomerizes under violet light (390-400nm) and dissociates under cyan light (490-500nm)

   Depositor

   Michael Lin

   Article

   Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

   Insert

   mNeptune2-Dronpa145N
   

   Expression

   Mammalian

   Mutation

   K145N on Dronpa (allows control of photochromic F…

   Promoter

   CMV

   Available Since

   Dec. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMKO.1 puro GFP shRNA

   Plasmid

   #10675

   Purpose

   Negative control vector for pMKO.1 puro (Addgene plasmid #8452); Contains shRNA against GFP.

   Depositor

   William Hahn

   Article

   Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

   Insert

   GFP shRNA
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Jan. 6, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMIG-Aalpha WT

   Plasmid

   #10884

   Depositor

   William Hahn

   Article

   Chen et al Cancer Res. 2005 Sep 15. 65(18):8183-92.

   Insert

   PP2A Aalpha (PPP2R1A Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. N-Myc-53BP1 WT pLPC-Puro

   Plasmid

   #19836

   Depositor

   Titia de Lange

   Article

   Dimitrova et al Nature. 2008 Nov 27;456(7221):524-8. doi

   Insert

   p53 Binding Protein 1 (TP53BP1 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Available Since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-EF1a-BbTagBY

   Plasmid

   #45185

   Depositor

   Dawen Cai, Joshua Sanes

   Article

   Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

   Has Service

   AAV9

   Insert

   pAAV-EF1a-Brainbow-invert tagBFP-EYFP-WPRE
   

   Use

   AAV; Adeno-associated viral

   Expression

   Mammalian

   Promoter

   human EF1a

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. PGKdtabpA

   Plasmid

   #13440

   Depositor

   Philippe Soriano

   Article

   Soriano et al Development. 1997 Jul . 124(14):2691-700

   Insert

   PGKdtabpA
   

   Expression

   Mammalian

   Available Since

   Nov. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti6-DEST PINK1-V5 KD

    Plasmid

    #13319

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 (PINK1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Kinase dead: K219A/D362A/D384A

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV5B-Smad4

    Plasmid

    #11743

    Depositor

    Jeff Wrana

    Article

    Macias-Silva et al Cell. 1996 Dec 27. 87(7):1215-24.

    Insert

    SMAD4 (Smad4 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 7, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pMJ841

    Plasmid

    #39318

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Aspartate 10 to Alanine (D10A) and Histidine 840 …

    Promoter

    T7

    Available Since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWPT-GFP

    Plasmid

    #12255

    Depositor

    Didier Trono

    Insert

    EGFP
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-FLEX-Arch-GFP

    Plasmid

    #22222

    Purpose

    Cre-dependent viral expression of Arch-GFP for neuronal inhibition

    Depositor

    Edward Boyden

    Article

    Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

    Has Service

    AAV1 and AAV9

    Insert

    Arch
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    no

    Promoter

    CAG

    Available Since

    Jan. 6, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. EF.hICN1.Ubc.GFP

    Plasmid

    #17626

    Depositor

    Linzhao Cheng

    Article

    Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

    Insert

    ICN1 (NOTCH1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVPT-tTR-KRAB

    Plasmid

    #11642

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    mPGK, GFP, tTR-KRAB, Tet-on
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCFJ350 - MCS(ttTi5605, II)

    Plasmid

    #34866

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

    Insert

    ttTi5605 targeting
    

    Use

    Worm targeting

    Available Since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQLinkH

    Plasmid

    #13667

    Depositor

    Konrad Buessow

    Article

    Scheich et al Nucleic Acids Res. 2007;35(6):e43. doi:

    Insert

    multi cloning site
    

    Use

    Co-expression

    Tags

    His, TEV, and attB1

    Expression

    Bacterial

    Available Since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGFP-HBx

    Plasmid

    #24931

    Depositor

    Xin Wang

    Article

    Forgues et al J Biol Chem. 2001 Jun 22. 276(25):22797-

    Insert

    HBx
    

    Tags

    EGFP and HA

    Expression

    Mammalian

    Available Since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pdCas9-DNMT3A-PuroR_v2

    Plasmid

    #74407

    Purpose

    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR_v2 (DNMT3A S. pyogenes, Synthetic, Human)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    CBh

    Available Since

    April 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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