We narrowed to 5,953 results for: 338

1. pcDNA3.1-SLC25A46[R257A,R347A]-P2A-Electra1 (pAH36)

   Plasmid

   #227620

   Purpose

   Overexpression of SLC25A46 with R247A and R347A mutations in mammalian cell lines

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Insert

   SLC25A46 (SLC25A46 Human)
   

   Use

   Tags

   P2A-mElectra1

   Expression

   Mammalian

   Mutation

   R257A,R347A

   Promoter

   CMV, T7

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICH86966::AtU6p::sgRNA:NbCysP6

   Plasmid

   #223217

   Purpose

   Expresses an sgRNA targeting the NbCysP6 gene in Nicotiana benthamiana with an Arabidopsis U6 promoter

   Depositor

   Tsepo Tsekoa

   Article

   Singh et al Front Plant Sci. 2022 Aug 18;13:953654.

   Insert

   NbCysP6 sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   None

   Expression

   Plant

   Mutation

   Promoter

   Arabidopsis U6 promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA.5.1-FHS-TNRC6A-SD-S1332D/S1346D/S1616D/S1691D

   Plasmid

   #215910

   Purpose

   Expression of TNRC6A-silencing domain (SD) with S1332D/S1346D/S1616D/S1691D mutation

   Depositor

   Kumiko Ui-Tei

   Article

   Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   Flag-HA-SBP

   Expression

   Mammalian

   Mutation

   contain aa1224-1709, S1332D, S1346D, S1616D, S169…

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA.5.1-FHS-TNRC6A-SD-S1332A/S1346A/S1616A/S1691A

   Plasmid

   #215911

   Purpose

   Expression of TNRC6A-silencing domain (SD) with S1332A/S1346A/S1616A/S1691A mutation

   Depositor

   Kumiko Ui-Tei

   Article

   Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   Flag-HA-SBP

   Expression

   Mammalian

   Mutation

   contain aa1224-1709, S1332A, S1346A, S1616A, S169…

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1-myc-GFP-TNRC6A-SD-S1332A/S1346A/S1616A/S1691A

   Plasmid

   #215905

   Purpose

   Expression of TNRC6A-silencing domain (SD) with S1332A/S1346A/S1616A/S1691A mutation

   Depositor

   Kumiko Ui-Tei

   Article

   Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   myc-GFP

   Expression

   Mammalian

   Mutation

   contain aa1224-1709, S1332A, S1346A, S1616A, S169…

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcs2-Δhel1/Δhel2HRI-GFP-IRES-mCherry

   Plasmid

   #226101

   Purpose

   HRI stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   HRI (EIF2AK1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   SIFI degron helices 1 & 2 deleted (Δ61-112)

   Promoter

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG_SRGAP2A_SH3(W765A)_GFP

   Plasmid

   #222616

   Purpose

   Expresses a form of full length SRGAP2 with one point mutation (W765A) in the SH3 domain abrogating its ability to bind to Gephyrin.

   Depositor

   Franck Polleux

   Article

   Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

   Insert

   SRGAP2 (SRGAP2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Point mutation W765A (SH3 domain)

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG_SRGAP2A_SH3(W765A)_HA

   Plasmid

   #222614

   Purpose

   Expresses a form of full length SRGAP2 with one point mutation (W765A) in the SH3 domain abrogating its ability to bind to Gephyrin.

   Depositor

   Franck Polleux

   Article

   Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

   Insert

   SRGAP2 (SRGAP2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Point mutation W765A (SH3 domain)

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG SRGAP2A_EVH1(P339L F342C)

   Plasmid

   #222613

   Purpose

   Expresses a form of full length SRGAP2 with two point mutations (P339L and F342C) that abrogate EVH1 binding site for Homer1

   Depositor

   Franck Polleux

   Article

   Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

   Insert

   SRGAP2 (SRGAP2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Point mutations P339L and F342C

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcs2-N-HRI(1-138)-GFP-IRES-mCherry

    Plasmid

    #226100

    Purpose

    HRI N-terminal region (residues 1-138) stability reporter construct for transient expression in mammalian cells

    Depositor

    Michael Rape

    Article

    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

    Insert

    HRI (EIF2AK1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains only N-HRI (aa 1-138)

    Promoter

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSuper DGK zeta human

    Plasmid

    #224575

    Purpose

    To downregulate the expression of human DGK zeta in human cells

    Depositor

    Isabel Mérida

    Article

    Avila-Flores et al Immunol Cell Biol. 2017 Jul;95(6):549-56

    Insert

    sh RNAi Diacylglycerol kinase zeta human (DGKZ Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    H1

    Available since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR-PDL1-LgBiT

    Plasmid

    #223619

    Purpose

    Lentiviral expression of human PD-L1 with LgBiT tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    PD-L1 (CD274 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    LgBiT

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330_NANOS3_cterm

    Plasmid

    #222904

    Purpose

    Cas9/sgRNA plasmid for targeting NANOS3

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    Cas9, NANOS3 sgRNA (NANOS3 Synthetic, Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. mouse b6 full length

    Plasmid

    #217816

    Purpose

    Full length mouse integrin b6 expression

    Depositor

    Timothy Springer

    Article

    Yuxin et al bioRxiv 2024.01.26.577394

    Insert

    ITGB6 (Itgb6 Mouse)
    

    Use

    Tags

    P2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. HRH2-DuET

    Plasmid

    #213313

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    HRH2 (HRH2 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GPR21-DuET

    Plasmid

    #213276

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPR21 (GPR21 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CD97-DuET

    Plasmid

    #213206

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CD97 (ADGRE5 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LENTICRISPR-LUC7L2_sgRNA1

    Plasmid

    #201602

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    LUC7L2 (LUC7L2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ACVR1-TASA-R206H

    Plasmid

    #206182

    Purpose

    in vitro transcription of RNA encoding human ACVR1 with mutations S190A, S194A, and R206H

    Depositor

    Mary Mullins

    Article

    Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

    Insert

    activin A receptor type 1 (ACVR1 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG tag

    Expression

    Mutation

    S190A, S194A, R206H

    Promoter

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ACVR1-TSAA-R206H

    Plasmid

    #206183

    Purpose

    in vitro transcription of RNA encoding human ACVR1 with mutations S192A, S194A, and R206H

    Depositor

    Mary Mullins

    Article

    Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

    Insert

    activin A receptor type 1 (ACVR1 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG tag

    Expression

    Mutation

    S192A, S194A, R206H

    Promoter

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge