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LLP781_SnoopCatcher-KRAB-CO

Plasmid

#211774

Purpose

SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional repressor KRAB, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

Traptavidin-KRAB

Use

Tags

3xV5

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect KRAB e…

Promoter

pEF1a and pSV40

Available since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG PSAM4 5HT3 LC IRES GFP

Plasmid

#123066

Purpose

Chemogenetic activator expression plasmid

Depositor

Scott Sternson

Article

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Insert

PSAM4 5HT3 LC IRES GFP (Htr3a Mouse)

Use

Tags

Expression

Mammalian

Mutation

L131G Q139L Y217F

Promoter

CAG

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pKRG3-mU6-PUb-hSpCas9-pAc

Plasmid

#162163

Purpose

Expresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectable

Depositor

Charles Rice

Article

Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

Inserts

hSpCas9
sgRNA

Use

CRISPR

Tags

T2A-pAc

Expression

Insect

Mutation

Promoter

Ae. aegypti PUb and Ae. aegypti U6

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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peSpCas9(1.1)-2×sgRNA (empty, donor)

Plasmid

#80768

Purpose

All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains eSpCas9(1.1) and two sgRNA expression cassettes. The first gRNA cloning site is empty.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pKRG3-mU6-PUb-3xFLAG-hSpCas9

Plasmid

#162161

Purpose

Expresses 3xFLAG tagged hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

Depositor

Charles Rice

Article

Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

Inserts

hSpCas9
sgRNA

Use

CRISPR

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

Ae. aegypti PUb and Ae. aegypti U6

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)

Plasmid

#71707

Purpose

A human codon-optimized SpCas9 fused to first 110 amino acids of human GEMININ and chimeric guide RNA expression plasmid

Depositor

Giulio Draetta

Article

Gutschner et al Cell Rep. 2016 Feb 3. pii: S2211-1247(16

Insert

humanized S. pyogenes Cas9 fused to human Geminin (GMNN Human)

Use

CRISPR

Tags

3xFLAG and hGEM(1/110)

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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NFATc3-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124288

Purpose

sgRNA against human NFATc3

Depositor

Csaba Vizler, Katalin Josvay

Insert

NFATc3 nuclear factor of activated T cells (NFATC3 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
NFATc4-CRISPR-gRNA#exon5-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124290

Purpose

sgRNA against huiman NFATc4

Depositor

Csaba Vizler, Katalin Josvay

Insert

NFATc4 nuclear factor of activated T cells (NFATC4 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
NFATc4-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124289

Purpose

sgRNA against human NFATc4

Depositor

Csaba Vizler, Katalin Josvay

Insert

NFATc4 nuclear factor of activated T cells (NFATC4 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
PX458-3xHA-SpCas9-HF1

Plasmid

#201952

Purpose

Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS D614G soluble Spike

Plasmid

#164651

Purpose

Expresses SARS-CoV-2 soluble Spike protein with a D614G mutation

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 soluble spike (S SARS-CoV-2)

Use

Tags

HIS

Expression

Mammalian

Mutation

D614G

Promoter

CAG

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only
pCAGGS/XBB.1.5-RBD

Plasmid

#213038

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/XBB.1.5-RBD (S )

Use

Tags

6 His Tag

Expression

Mammalian

Mutation

G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

Promoter

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAS9-mCherry-Frame +0

Plasmid

#66939

Purpose

CRISPaint frame selector +0

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

gRNA frame +0

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCAS9-mCherry-Frame +1

Plasmid

#66940

Purpose

CRISPaint frame selector +1

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

gRNA frame +1

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-RSV-SpCas9

Plasmid

#85450

Purpose

Express SpCas9 in mammalian cells

Depositor

Hetian Lei

Article

Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

Insert

pRSV

Use

AAV

Tags

Expression

Mutation

Promoter

pRSV

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCAS9-mCherry-Frame +2

Plasmid

#66941

Purpose

CRISPaint frame selector +2

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

gRNA frame +2

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pMUT2-pCaf-mCherry

Plasmid

#192860

Purpose

pMUT2 based vector for sensing caffeine in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

Nanobody-CadC fusion protein and cognate promoter inducible by caffeine

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.pU1a-SpCas9

Plasmid

#121507

Purpose

Expresses codon-optimized SpCas9 in mammalian cells.

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

SpCas9

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

U1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG/hTAZ-FL

Plasmid

#139979

Purpose

Expresses full length human Tafazzin with codon modified for cloning. Transgene flanked by AAV2 ITR

Depositor

William Pu

Article

Wang et al Circ Res. 2020 Mar 9. doi: 10.1161/CIRCR

Insert

Full length human Tafazzin (TAFAZZIN Human)

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCA528 CAPN7 (1-75)

Plasmid

#193031

Purpose

Bacterial expression plasmid for CAPN7 (1-75) N-terminal MIT domain

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

CAPN7 (CAPN7 Human)

Use

Tags

6xHis-Sumo

Expression

Bacterial

Mutation

Residues 1-75

Promoter

T7

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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