We narrowed to 18,749 results for: REV

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AAVS1 EGFP-GABARAPL1 HRD

Plasmid

#207552

Purpose

Homologous recombination donor to integrate and expression cassette for EGFP-GABRAPL1 in the AAVS1 locus of human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

TET inducible EGFP-GABARAPL1

Tags

EGFP

Expression

Mammalian

Promoter

Endogenous

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pDA077

Plasmid

#131388

Purpose

L1RP expression

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

L1RP

Expression

Mammalian

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt11

Plasmid

#35922

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt11 (wnt11f2 Human)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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EKAREN5-gl

Plasmid

#225958

Purpose

The YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.

Depositor

Toru Hiratsuka

Article

Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.

Insert

EKAREN5-gl

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Promoter

EF-1-alpha promoter

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pGSKU

Plasmid

#72243

Purpose

Contains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoter

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

Inserts

KlURA3
kanMX4
I-SceI

Expression

Bacterial

Available Since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only

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TJP1_PDZ_2

Plasmid

#103910

Purpose

Protein expression and purification of ZO1 PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

TJP1 (TJP1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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TJP1_PDZ_1

Plasmid

#103909

Purpose

Protein expression and purification of ZO1 PDZ1 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

TJP1 (TJP1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-puro-kozak-scFv-GCN4-HAtag-msfGFP(V206K)-GBI

Plasmid

#205162

Purpose

express scFv-GCN4-HAtag-msfGFP(V206K)-GBI

Depositor

Weirui Ma

Article

Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

Insert

scFv-GCN4-HAtag-msfGFP(V206K)-GBI

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLAG-APEX2-TAX1BP1

Plasmid

#175761

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-TAX1BP1

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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FRP1642_PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

Plasmid

#58442

Purpose

Cassette to replace yeast promoters with an inducible promoter containing 4 lexA boxes

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

Expression

Yeast

Promoter

PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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AAVS1 EGFP-Sec61B

Plasmid

#207553

Purpose

Homologous recombination donor to integrate and expression cassette for EGFP-Sec61B in the AAVS1 locus of human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

TET inducible EGFP-Sec61B

Tags

EGFP

Expression

Mammalian

Promoter

Endogenous

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Rho*-BERKY3

Plasmid

#158220

Purpose

BRET biosensor for detection of Rho-GTP in mammalian expression vector pcDNA3.1(+)

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-Rhotekin’s RBD (amino acids 7-89)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 19, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pJFRC201-10XUAS-FRT>STOP>FRT-myr::smGFP-HA

Plasmid

#63166

Purpose

GAL4-responsive UAS "flp-On" spaghetti monster GFP-HA reporter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-HA"

Tags

N-myristoylation

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-3HA-His3MX6

Plasmid

#41600

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

His3

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Available Since

May 15, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLExFRT mGFP-2A-Synaptophysin-mRuby

Plasmid

#71761

Purpose

mGFP and Synaptophysin-mRuby expression from Flp-expressing neurons

Depositor

Liqun Luo

Article

Beier et al Cell. 2015 Jul 30;162(3):622-34. doi: 10

Inserts

mGFP
Synaptophysin

Use

AAV

Tags

Palmitoylation signal and mRuby

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-TAX1BP1

Plasmid

#175766

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-mCherry-raptor

Plasmid

#73386

Purpose

Expresses raptor tagged with mCherry and HA

Depositor

Jie Chen, Taekjip Ha

Article

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

Regulatory Associated Protein Of MTOR, Complex 1 (RPTOR Human)

Tags

HA and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pZS165 CRISPEY HH-HDV NotI

Plasmid

#114451

Purpose

Yeast Centromere Plasmid for galactose inducible expression of retron-guide chimeric RNA with flanking HH-HDV ribozyme

Depositor

Hunter Fraser

Article

Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

Type

Empty backbone

Use

CRISPR; Cen/ars

Expression

Yeast

Promoter

ScGAL7p

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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Vinculin-venus

Plasmid

#27300

Depositor

Martin Schwartz

Article

Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

Insert

Vinculin (VCL Chicken)

Tags

Venus A206K

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pANCH2-Nat

Plasmid

#87249

Purpose

gene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT)

Depositor

Kerstin Bystricky

Article

Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

Insert

Nat

Expression

Yeast

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pBD-pZ-FLAG

Plasmid

#183148

Purpose

Yeast-two-hybrid BD vector with pZ promoter (activated by ZPM)

Depositor

Assaf Mosquna

Article

Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

Insert

GAL4 BD

Use

Yeast two-hybrid

Tags

FLAG

Expression

Yeast

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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SpCas9

Plasmid

#188489

Purpose

Expresses FLAG tagged SpCas9

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTagBFP2-C1-lynN11-FKBP-PI-PLCN187

Plasmid

#139308

Purpose

N-terminal half of split PI-PLC targeted to plasma membrane

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

LYN(1-11):RSANS[GA]4:TagBFP2: FKBP1A(3-108): [GGSA]4GG:L. monocytogenes PI-PLC(1-187)

Expression

Mammalian

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-PPARgamma2

Plasmid

#78768

Purpose

To overexpress PPARgamma2 in Mammalian Cells

Depositor

Jaewhan Song

Article

Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

Insert

PPARG (Pparg Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
IRE1-2EGFP-ter-kanMX4

Plasmid

#184766

Purpose

Integration of 2xGFP tag at IRE1 C terminus. Potential indicator of UPR activation. Uses antibiotic resistance marker kanMX4.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

IRE1 (IRE1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pTopo_Ex2Donor_pPGK-PURO

Plasmid

#198862

Purpose

Donor template for CRISPR/Cas9 targeting of TERT Ex2

Depositor

Agnel Sfeir

Article

Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

Insert

PURO

Expression

Mammalian

Promoter

PGK

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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SpCas9 nickase for Split-PE (pJUL2497)

Plasmid

#190104

Purpose

Expresses nickase SpCas9(H840A) for Split-PE use

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

nSpCas9

Tags

XTEN-bpNLS and bpNLS

Expression

Mammalian

Mutation

H840A

Promoter

CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pACT-Lex

Plasmid

#183149

Purpose

Yeast-two-hybrid AD vector with 4XLexOp (activated by Lex-ER-B112)

Depositor

Assaf Mosquna

Article

Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

Insert

GAL4 AD

Use

Yeat two-hybrid

Tags

HA tag

Expression

Yeast

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pJ3H PTP-1B

Plasmid

#8601

Depositor

Ben Neel

Article

Venable et al J Biol Chem. 2000 Jun 16. 275(24):18318-

Insert

PTP-1B (PTPN1 Human)

Tags

HA

Expression

Mammalian

Mutation

K103R

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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AsCas12a

Plasmid

#188492

Purpose

Expresses FLAG tagged AsCas12a

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag Rac2

Plasmid

#12192

Depositor

Gary Johnson

Article

Abell et al J Cell Sci. 2004 Jan 15. 117(Pt 2):243-5

Insert

Rac2 (RAC2 Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-mIFITM3

Plasmid

#58389

Purpose

Expressed murine IFITM3 with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV IE

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only
pAID6

Plasmid

#136989

Purpose

Yeast integration plasmid for expression of dLbCpf1-VP, dSpCas9-RD1152, SaCas9, and Csy4

Depositor

Huimin Zhao

Article

Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

Inserts

dLbCpf1-VP
dSpCas9-RD1152
SaCas9
Csy4

Expression

Yeast

Promoter

ENO2, TDH3, TEF1, and TPI1

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pDC191

Plasmid

#115792

Purpose

Expresses Rat synaptobrevin 2

Depositor

Axel Brunger

Article

Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

Insert

Synaptobrevin2 (Vamp2 Rat)

Tags

hexa histidine

Expression

Bacterial

Mutation

S28C and C103S (please see comments below)

Promoter

T7

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-F30-Tornado-Acyclovir-Broccoli#4

Plasmid

#216822

Purpose

Expresses acyclovir-controllable Broccoli aptamer #4 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Acyclovir-Broccoli#4

Expression

Mammalian

Promoter

U6+27

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-basP:fLuc-TK:rLuc

Plasmid

#138369

Purpose

Lentiviral plasmid contains basal promoter driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoter

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

sequence multi cloning sites, minP driving firefly luciferase and TK promoter driving renilla luciferase

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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smHA multi-frame tag

Plasmid

#128611

Purpose

a spaghetti monster with 10x HA epitopes repeat upstream of the HIV frameshift sequence which is followed by FLAG epitopes in the 0ORF are separated from one another by SunTag epitopes in the -1ORF.

Depositor

Tim Stasevich

Article

Lyon et al Mol Cell. 2019 Jul 11;75(1):172-183.e9.

Insert

smHA MF tag

Expression

Mammalian

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pACEBac1-GCP2

Plasmid

#178068

Purpose

Insect cell expression of GCP2

Depositor

Tarun Kapoor

Article

Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

Insert

GCP2 (TUBGCP2 Human)

Expression

Insect

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AAVS1 LAMP1-mNeonGreen HRD

Plasmid

#207549

Purpose

Homologous recombination donor to integrate and expression cassette for LAMP1-mNEONGREEN in the AAVS1 locus of human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

TET inducible LAMP1-mNeonGreen

Tags

mNeonGreen

Expression

Mammalian

Promoter

Endogenous

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-AAVS1_sgRNA

Plasmid

#183891

Purpose

pX459V2.0-HypaCas9 plasmid with sgRNA targeting the AAVS1 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

AAVS1 sgRNA spacer (AAVS1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 18,749 results for: REV