We narrowed to 26,826 results for: STI

1. Nephrin

   Plasmid

   #186134

   Purpose

   For expression of recombinant nephrin in bacteria cells

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   NPHS1 (NPHS1 Human)
   

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Amino acid residues 1174-1223, with mutations: Y1…

   Promoter

   Lac

   Available Since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCK760

   Plasmid

   #192643

   Purpose

   Expresses BBa_J23110-sfGFP (capacity monitor) and J3-Bba_J23117-mRFP (CRISPRa reporter) on pSC101-AmpR

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

   Insert

   BBa_J23110-sfGFP
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   BBa_J23110

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJH3185

   Plasmid

   #132367

   Purpose

   Prgef-1 GFP::DAF-16a unc-54 3' UTR C.elegans pan-neural expression of isoform a of DAF-16 fused to GFP

   Depositor

   Mei Zhen

   Article

   Hung et al Development. 2014 Apr;141(8):1767-79. do

   Insert

   DAF-16a (daf-16 Nematode)
   

   Tags

   GFP

   Expression

   Worm

   Promoter

   Prgef-1

   Available Since

   Oct. 28, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pKF-P14MM2AG5h

   Plasmid

   #128254

   Purpose

   Mammalian positive feedback synthetic gene circuit within a Flp-In expression vector.

   Depositor

   Gabor Balazsi

   Article

   Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

   Insert

   rtTA-Advanced::P2A::EGFP
   

   Use

   Synthetic Biology; Flp-in expression vector

   Expression

   Mammalian

   Promoter

   tight TRE promoter

   Available Since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNICE HA-mSYD1A

   Plasmid

   #59361

   Purpose

   Expression plasmid for HA-tagged mSYD1A / Syde1 (HA-tag at N-terminus of mSYD1A)

   Depositor

   Peter Scheiffele

   Article

   Wentzel et al Neuron. 2013 Jun 19;78(6):1012-23. doi:

   Insert

   mSYD1A (Syde1 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEPQD0CM0068

   Plasmid

   #177025

   Purpose

   Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   major latex protein-like
   

   Use

   Synthetic Biology

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. FRB-polySH33-EGFP

   Plasmid

   #175792

   Purpose

   Bacterial expression and purification, fluorescently tagged scaffold for droplet visualization and volume quantification

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   NCK1 (NCK1 Human)
   

   Tags

   His6 and MBP

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX311 LacZ

   Plasmid

   #117731

   Purpose

   Open reading frame vector encoding LacZ

   Depositor

   William Hahn

   Article

   Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

   Insert

   LacZ
   

   Use

   Lentiviral

   Tags

   2X V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFIN-XOPS-tdTOM

   Plasmid

   #44359

   Depositor

   Susan Semple-Rowland

   Article

   Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

   Insert

   XOPS-tdTOM
   

   Use

   Lentiviral

   Promoter

   Xenopus Opsin (XOPS) (pos 3125-4501)

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. PCTAIRE-1

    Plasmid

    #1996

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    PCTAIRE-1 (CDK16 Human)
    

    Expression

    Mammalian

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

11. EFS eGFP hPGK BSD

    Plasmid

    #188520

    Purpose

    Backbone for CRISPR interference (CRISPRi) isogenic PAM testing

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSW178 - pXPP

    Plasmid

    #115987

    Purpose

    entry vector for GreenGate cloning method, promoter

    Depositor

    Alexis Maizel

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pXPP
    

    Use

    Unspecified

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. p667-UBC-AAK1-V5-miniTurbo_IDG-K

    Plasmid

    #135255

    Purpose

    Gateway destination clone of AAK1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    AAK1-V5-miniTurbo (AAK1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAS1_4x7SKPylT_EF1_Ub-BsmBI_IRES_BSD

    Plasmid

    #162804

    Purpose

    STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-BsmBI_IRES_Blasticidin
    

    Use

    Piggybac

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Blat-BSD

    Plasmid

    #163791

    Purpose

    The plasmid expresses human codon-optimized BlatCas9, gRNA expression elements and blasticidin resistence gene.

    Depositor

    Yongming Wang

    Article

    Gao et al Front Cell Dev Biol. 2020 Oct 19;8:58316

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-HA-ERK2 Y261A

    Plasmid

    #8977

    Depositor

    John Blenis

    Article

    Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

    Insert

    ERK2 (Mapk1 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Y261A

    Available Since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

17. pUM31dao

    Plasmid

    #87242

    Purpose

    Plastid expression cassette containing Schizosaccharomyces pombe D-amino acid oxidase gene. Provides dual positive (D-alanine) and negative (D-valine) selection in transplastomic plants

    Depositor

    Anil Day

    Article

    Gisby et al Plant Physiol. 2012 Dec;160(4):2219-26.

    Insert

    D-amino oxidase
    

    Expression

    Plant

    Promoter

    plastid rrn

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBJ1807 pRS415-COF1RFP

    Plasmid

    #37102

    Depositor

    John Cooper

    Article

    Lin et al J Cell Sci. 2010 Apr 15;123(Pt 8):1329-4

    Insert

    COF1-RFP
    

    Tags

    RFP

    Expression

    Yeast

    Mutation

    Single RFP internal insertion at Cof147T48D with …

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3-HA-ERK2 L232A

    Plasmid

    #8976

    Depositor

    John Blenis

    Article

    Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

    Insert

    ERK2 (Mapk1 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    L232A

    Available Since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pNICE HA-mSYD1B

    Plasmid

    #59362

    Purpose

    Expression plasmid for HA-tagged mSYD1B / Syde2 (HA-tag at N-terminus of mSYD1B)

    Depositor

    Peter Scheiffele

    Article

    Wentzel et al Neuron. 2013 Jun 19;78(6):1012-23. doi:

    Insert

    mSYD1B (Syde2 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCK671.306

    Plasmid

    #192641

    Purpose

    Expresses dxCas9-NG and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Insert

    J306 scRNA
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    BBa_J23119

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCAG-attBr-Cre-rc-attP

    Plasmid

    #51264

    Purpose

    Component of the Bxb1-rcCre binary site-specific recombinase system, reverse complementary Cre CDS flanked by Bxb1 attB/P recognition sites in head-to-head orientation

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    attBr-Cre-rc-attP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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23. DIRAS3

    Plasmid

    #55686

    Purpose

    expression of human DIRAS3

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    DIRAS3 (DIRAS3 Human)
    

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

24. XE117 CAMKII-CS2+

    Plasmid

    #16737

    Depositor

    Randall Moon

    Article

    Kühl et al J Biol Chem. 2000 Apr 28. 275(17):12701-

    Insert

    CAMKII (Camk2a Rat)
    

    Use

    Xenopus expression

    Available Since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

25. p663-UBC-miniTurbo-V5-ADCK1_IDG-K

    Plasmid

    #158195

    Purpose

    Gateway destination clone of ADCK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-ADCK1 (ADCK1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p663-UBC-miniTurbo-V5-CAMKK1_IDG-K

    Plasmid

    #158169

    Purpose

    Gateway destination clone of CAMKK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CAMKK1 (CAMKK1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. wt1aCreERT2

    Plasmid

    #108464

    Purpose

    Used to generate a cryst:mCherry wt1a:CreERT2 zebrafish transgenic line.

    Depositor

    Nadia Mercader Huber

    Article

    Sanchez-Iranzo et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

    Insert

    wt1aCreERT2
    

    Use

    Cre/Lox

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p663-UBC-miniTurbo-V5-CDK10_IDG-K

    Plasmid

    #158171

    Purpose

    Gateway destination clone of CDK10 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CDK10 (CDK10 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCS2-Opto-Alk8

    Plasmid

    #207615

    Purpose

    Zebrafish Alk8 BMP receptor kinase domain fused to VfLOV

    Depositor

    Patrick Müller

    Article

    Rogers et al Elife. 2020 Nov 11;9:e58641. doi: 10.755

    Insert

    Alk8 (acvr1l) kinase domain + VfLOV domain (acvr1l Zebrafish, Vaucheria frigida)
    

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCTCON2-GYB

    Plasmid

    #160927

    Purpose

    Dual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (wild-type construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Inserts

    BFP
    sfGFP
    

    Expression

    Yeast

    Promoter

    GAL1-10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Cdk3-DN-HA

    Plasmid

    #1880

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    Cdk3 (CDK3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Dominant negative, D to N in conserved KLAD*FGLAR…

    Available Since

    Jan. 24, 2006

    Availability

    Academic Institutions and Nonprofits only

32. p663-UBC-miniTurbo-V5-STK3_IDG-K

    Plasmid

    #189879

    Purpose

    Gateway destination clone of STK3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    STK3 (STK3 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pFIN-EF1a-GFP-WPRE

    Plasmid

    #44352

    Depositor

    Susan Semple-Rowland

    Article

    Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

    Insert

    EF1a-GFP
    

    Use

    Lentiviral

    Promoter

    human elongation factor -1a (pos 2526-3969)

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBJ1808 pRS416-COF1RFP

    Plasmid

    #37103

    Depositor

    John Cooper

    Article

    Lin et al J Cell Sci. 2010 Apr 15;123(Pt 8):1329-4

    Insert

    COF1-RFP
    

    Tags

    RFP

    Expression

    Yeast

    Mutation

    Single RFP internal insertion at Cof147T48D with …

    Available Since

    June 19, 2012

    Availability

    Academic Institutions and Nonprofits only

35. pCMV-NES-MBP-cATOM(Ras)

    Plasmid

    #209711

    Purpose

    Mammalian expression of c-ATOM biosensor for HRas with nuclear export signal

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    NES-MBP-cATOM(Ras)
    

    Tags

    NES-MBP

    Expression

    Mammalian

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. JC101: pMVP/Ad/PK-DEST

    Plasmid

    #121845

    Purpose

    pMVP destination vector, empty pK fiber-modified adenovirus vector backbone

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Adenoviral; Pmvp gateway destination vector

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. p667-UBC-MAP3K14-V5-miniTurbo_IDG-K

    Plasmid

    #189903

    Purpose

    Gateway destination clone of MAP3K14 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    MAP3K14 (MAP3K14 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pWZL-Stat5a1*6

    Plasmid

    #130669

    Purpose

    Retroviral vector for the expression of constitutive Stat5a (hygromycin resistance)

    Depositor

    Gerardo Ferbeyre

    Article

    Mallette et al J Biol Chem. 2007 Nov 30;282(48):34938-4

    Insert

    Stat5a (Stat5a Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    changed Histidine 298 to Arginine and Serine 710 …

    Promoter

    LTR

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRS416-Aga1p-FAPB2.3.6

    Plasmid

    #158147

    Purpose

    Yeast display reporter for non-display yeast strains (wild-type construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    Aga1p-FAPB2.3.6
    

    Expression

    Yeast

    Promoter

    GAL1-10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pHR-SIN-GFP-PTEN-G129E

    Plasmid

    #30544

    Depositor

    Todd Waldman

    Article

    Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

    Insert

    GFP-PTEN-G129E (PTEN Human)
    

    Expression

    Mammalian

    Mutation

    GFP-PTEN-G129E

    Available Since

    Aug. 11, 2011

    Availability

    Academic Institutions and Nonprofits only