Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 79,968 results for: Rest

Clear keywords
Showing: 9501 - 9550 of 79968 results

  1. pLenti_P2A_Blast

    Plasmid
    #211365
    Purpose
    Lentiviral expression vector for an insert of interest linked to a P2A-T2A blasticidin sequence. Produces virus very efficiently. Can also be used for regular transfections
    Depositor
    Jacques Neefjes
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Jan. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    211365-plasmid-map-sequence-id-437557

    Enlarge

  3. pSpCas9(BB)-2A-Hygro

    Plasmid
    #127763
    Purpose
    Used for expression of sgRNA in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    June 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127763-plasmid-map-sequence-id-247560

    Enlarge

  4. tet-pLKO.puro_shLuc

    Plasmid
    #110422
    Purpose
    shLuc control, silence Luc gene, doxycycline inducible, puromycin selection
    Depositor
    Sandra Martha Gomes Dias
    Insert
    Luciferase
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    H1/TO (RNA PolIII)
    Available Since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    110422-plasmid-map-sequence-id-213315

    Enlarge

  5. pEGFP-centrin-1

    Plasmid
    #72641
    Purpose
    The plasmid encodes centrin-1 with an N-terminal EGFP tag
    Depositor
    Michel Bornens
    Insert
    centrin-1 (CETN1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72641-plasmid-map-sequence-id-132790

    Enlarge

  6. pCDH-EF1-IRES eGFP

    Plasmid
    #128059
    Purpose
    Lentiviral expression of an insert from EF1 promoter and co-expression of EGFP. See Depositor Comments for additional information regarding plasmid sequence.
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Nov. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    128059-plasmid-map-sequence-id-256675

    Enlarge

  8. pAAV-CaMKIIa-EGFP

    Plasmid
    #50469
    Purpose
    EGFP under the control of CaMKIIa promoter
    Depositor
    Bryan Roth
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    EGFP
    Use
    AAV
    Tags
    N/A
    Promoter
    CaMKIIa
    Available Since
    March 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    50469-plasmid-map-sequence-id-171178

    Enlarge

  10. pLV-EBFP2-nuc

    Plasmid
    #36085
    Depositor
    Pantelis Tsoulfas
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Replace EYFP of the vector pEYFP-Nuc from Clontec…
    Available Since
    June 1, 2012
    Availability
    Academic Institutions and Nonprofits only
    36085-plasmid-map-sequence-id-44618

    Enlarge

  11. NUDT1

    Plasmid
    #74660
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    NUDT1 (NUDT1 Human)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  12. hIRE1a.pcD

    Plasmid
    #21892
    Depositor
    Randal Kaufman
    Insert
    IRE1a (ERN1 Human)
    Expression
    Mammalian
    Available Since
    Sept. 10, 2009
    Availability
    Academic Institutions and Nonprofits only
    21892-plasmid-map-sequence-id-10067

    Enlarge

  14. pTCF156 HLA-A*02:01

    Plasmid
    #180456
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-A*02:01
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available Since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    180456-plasmid-map-sequence-id-355799

    Enlarge

  15. pTCF158 Human beta-2 microglobulin

    Plasmid
    #180457
    Purpose
    E. coli expression of class I MHC light chain
    Depositor
    John Altman
    Insert
    Human beta-2 microglobulin
    Expression
    Bacterial
    Available Since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    180457-plasmid-map-sequence-id-355801

    Enlarge

  17. pHH0103 C20orf67 WW domain

    Plasmid
    #104273
    Purpose
    Bacterial expression of WW domain from C20orf67
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    C20orf67 WW domain (PCIF1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104273-plasmid-map-sequence-id-203480

    Enlarge

  18. AAV-CAG-jGCaMP8m-WPRE

    Plasmid
    #179255
    Purpose
    AAV-mediated expression of GCaMP8m under the CAG promoter
    Depositor
    Loren Looger
    Has Service
    AAV Retrograde, AAV1, and AAV9
    Insert
    jGCaMP8m
    Use
    AAV
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Feb. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    179255-plasmid-map-sequence-id-354032

    Enlarge

  19. hTLR2 flag

    Plasmid
    #13082
    Depositor
    Ruslan Medzhitov
    Insert
    TLR2 (TLR2 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Amino acids 1-16 have been replaced with mammalia…
    Available Since
    Oct. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13082-plasmid-map-sequence-id-142444

    Enlarge

  20. pICSL12013

    Plasmid
    #245630
    Purpose
    Level 0 Golden Gate part 6GAL4UAS promoter, GGAG - AATG
    Depositor
    Mark Youles
    Insert
    6GAL4UAS promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245630-plasmid-map-sequence-id-495460

    Enlarge

  21. XLone-PE2

    Plasmid
    #136463
    Purpose
    Expresses prime editing 2 (PE2) enzyme upon doxycycline treatment
    Depositor
    Xiaojun Lian
    Insert
    PE2
    Expression
    Mammalian
    Promoter
    TRE3GS
    Available Since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    136463-plasmid-map-sequence-id-272540

    Enlarge

  22. GSK3 beta pGEX

    Plasmid
    #15898
    Depositor
    Jim Woodgett
    Insert
    GSK3 beta (GSK3B Human)
    Tags
    GST
    Expression
    Bacterial
    Available Since
    Nov. 1, 2007
    Availability
    Industry, Academic Institutions, and Nonprofits
    15898-plasmid-map-sequence-id-413253

    Enlarge

  24. Pet28a_Human_H3.1

    Plasmid
    #42631
    Depositor
    Joe Landry
    Insert
    Human H3.1 (H3C1 Human)
    Tags
    Untagged
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 25, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    42631-plasmid-map-sequence-id-57746

    Enlarge

  25. p75-RFP

    Plasmid
    #24092
    Depositor
    Moses Chao
    Insert
    p75NTR (NGFR Human)
    Tags
    DSRed
    Expression
    Mammalian
    Available Since
    Jan. 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    24092-plasmid-map-sequence-id-473506

    Enlarge

  26. pX551-miniCMV-SpCas9

    Plasmid
    #107031
    Purpose
    AAV plasmid expressing Cas9 under control of miniCMV promoter
    Depositor
    Alex Hewitt
    Insert
    SpCas9
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Available Since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107031-plasmid-map-sequence-id-208545

    Enlarge

  27. mTLR4 flag

    Plasmid
    #13087
    Depositor
    Ruslan Medzhitov
    Insert
    TLR4 (Tlr4 Mouse)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Amino acids 1-19 have been replaced with mammalia…
    Available Since
    Oct. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13087-plasmid-map-sequence-id-511101

    Enlarge

  28. pCFD3.1-w-dU6:3gRNA

    Plasmid
    #123366
    Purpose
    gRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).
    Depositor
    Simon Bullock
    Insert
    U6:3-gRNA
    Use
    CRISPR
    Expression
    Insect
    Available Since
    April 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    123366-plasmid-map-sequence-id-242548

    Enlarge

  30. 1257 pGEX4T3 GST FKHR

    Plasmid
    #10706
    Depositor
    William Sellers
    Insert
    FKHR (FOXO1 Human)
    Tags
    GST
    Expression
    Bacterial
    Available Since
    March 14, 2006
    Availability
    Academic Institutions and Nonprofits only

  32. PME-1 S156A (inakt.mut) pcDNA-StrepIII

    Plasmid
    #119293
    Purpose
    Mammalian overexpression of StrepII-tagged full length PPME1 with mutation in the methylesterase active center
    Depositor
    Jukka Westermarck
    Insert
    PPME1 (PPME1 Human)
    Expression
    Mammalian
    Mutation
    S156A
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    119293-plasmid-map-sequence-id-232194

    Enlarge

  33. pAAV-syn-DIO-axon-jGCaMP8f

    Plasmid
    #220531
    Purpose
    For Cre-dependent enriched expression of GCaMP8f in axons
    Depositor
    Lin Tian
    Insert
    Axon-DIO-GCaMP8f
    Use
    AAV
    Expression
    Mammalian
    Promoter
    hSynapsin
    Available Since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    220531-plasmid-map-sequence-id-436147

    Enlarge

  34. pJK615

    Plasmid
    #71699
    Purpose
    Produces E. coli thioredoxin with a C-terminal His6 tag (EcTrxAH6)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71699-plasmid-map-sequence-id-137034

    Enlarge

  37. pREP-NFAT2

    Plasmid
    #11788
    Depositor
    Anjana Rao
    Insert
    NFAT2 (NFATC1 Human)
    Expression
    Mammalian
    Available Since
    May 25, 2006
    Availability
    Academic Institutions and Nonprofits only
    11788-plasmid-map-sequence-id-323906

    Enlarge

  38. FLAG-Foxo4

    Plasmid
    #17549
    Depositor
    Domenico Accili
    Insert
    FoxO4 (FOXO4 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Available Since
    Oct. 20, 2008
    Availability
    Academic Institutions and Nonprofits only
    17549-plasmid-map-sequence-id-176085

    Enlarge

  39. pSRG101 (KasI::24xGCN4::T2A::AvrII)

    Plasmid
    #246458
    Purpose
    24xGCN4::T2A with flanking restriction sites (KasI and AvrII). Useful for cloning SunTag reporter plasmids for expression in C. elegans.
    Depositor
    Suzan Ruijtenberg
    Insert
    24xGCN4::T2A
    Expression
    Bacterial
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    246458-plasmid-map-sequence-id-496351

    Enlarge

  40. pOpen-T5gene12/13

    Plasmid
    #165559
    Purpose
    Double-stranded DNA specific exonuclease and single-stranded DNA endonuclease. Initiates at the 5' termini of linear or nicked double-stranded DNA. Cleaves linear or nicked double-stranded DNA in the 5' to 3' direction.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    T5 Exonuclease
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165559-plasmid-map-sequence-id-339952

    Enlarge

  41. pT7-V5-SBP-C1-HsPTB1

    Plasmid
    #64925
    Purpose
    Expresses V5- and SBP-tagged PTB1 in mammalian cells
    Depositor
    Elisa Izaurralde
    Insert
    PTB1 (PTBP1 Human)
    Tags
    V5 and SBP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    64925-plasmid-map-sequence-id-109671

    Enlarge

  42. pT7-V5-SBP-C1-HshnRNPE1

    Plasmid
    #64921
    Purpose
    Expresses V5- and SBP-tagged hnRNPE1 in mammalian cells
    Depositor
    Elisa Izaurralde
    Insert
    hnRNPE1 (PCBP1 Human)
    Tags
    V5 and SBP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    64921-plasmid-map-sequence-id-109667

    Enlarge

  45. pOpen-T4gene30

    Plasmid
    #165554
    Purpose
    Ligase derived from T4 bacteriophage expressed in the early stage of lytic development. Has been implicated in T4 DNA synthesis and genetic recombination. May also play a role in T4 DNA repair.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    T4-DNA Ligase
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165554-plasmid-map-sequence-id-339904

    Enlarge

  47. pGAPZa-STE2-1D4

    Plasmid
    #133422
    Purpose
    Mutant form of yeast STE2 coding sequence in vector for protein expression in the methanogenic yeast Pichia pastoris, with GAP promoter.
    Depositor
    Gerhard Wagner
    Insert
    STE2 (STE2 Budding Yeast)
    Expression
    Yeast
    Mutation
    C59S, C252A
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    133422-plasmid-map-sequence-id-267645

    Enlarge

  48. pSpCas9(BB)-2A-miRFP670

    Plasmid
    #91854
    Purpose
    Cas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988
    Depositor
    Ralf Kuehn
    Inserts
    hSpCas9-2A-miRFP670
    BB-guide RNA
    Use
    CRISPR
    Tags
    2A-miRFP670 and 3XFLAG
    Expression
    Mammalian
    Promoter
    Cbh and U6
    Available Since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    91854-plasmid-map-sequence-id-176529

    Enlarge

  49. Pet28a_Synthetic_Human_H2A.1

    Plasmid
    #42634
    Depositor
    Joe Landry
    Insert
    Human H2A.1 (H2AC13 Synthetic, Human)
    Tags
    Untagged
    Expression
    Bacterial
    Mutation
    Codon Optimized
    Promoter
    T7
    Available Since
    Feb. 25, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    42634-plasmid-map-sequence-id-57749

    Enlarge

  50. pFA6a-link-ymScarletI3-URA3

    Plasmid
    #224075
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with URA3 selection
    Depositor
    Bas Teusink
    Insert
    ymScarletI3
    Tags
    ymScarletI3
    Expression
    Yeast
    Available Since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    224075-plasmid-map-sequence-id-460510

    Enlarge

Showing: 9501 - 9550 of 79968 results