We narrowed to 8,201 results for: Gal
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Plasmid#159402PurposeConstitutive murine TrkB expression in mammalian host, controlled under cytomegalovirus (CMV) promoter and enhancerDepositorInsertpLenti-CMV-TrkB (Ntrk2, Mouse) (Ntrk2 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterCMV promoterAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
MmSphk1
Plasmid#118599PurposeExpresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in miceDepositorInsertMus musculus sphingosine kinase 1
UseTagsFLAGExpressionYeastMutationPromoterGAL1, 10Available SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
HsSPHK1
Plasmid#118091PurposeExpresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)DepositorInsertHomo sapiens Sphingosine Kinase 1
UseTagsFLAGExpressionYeastMutationPromoterGAL1, 10Available SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-RosaL6 ELD mutations
Plasmid#37198DepositorInsertFokI (fused to Rosa L6 ZFN)
UseZfn expressionTagsHA, ROSA26 eZFNs L6, and SV40 NLSExpressionMammalianMutationQ486E, I499L and N496DPromoterCMVAvailable SinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-R4-uAb
Plasmid#101800PurposeMammalian cell expression of the beta-galactosidase-specific ubiquibody, R4-uAbDepositorInsertR4-uAb
UseTags6xHis and FlagExpressionMammalianMutationPromoterCMVAvailable SinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BYG
Plasmid#158126PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)DepositorInsertmTagBFP
UseTagsExpressionYeastMutationPromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
P305-FBP_4
Plasmid#139695PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathwayDepositorInsertpLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:tAtuOCS, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab5 WT
Plasmid#53468DepositorAvailable SinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTi4.0_SpdCas9_pmcDA1
Plasmid#204613PurposeCRISPR cytosine deaminase integrative plasmid for Mycoplasma gallisepticumDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable SinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF-ENTR-LacZ (710-1)
Plasmid#17430PurposeGateway entry vector with EF1-a promoter, V5-His C-term, Lac ZDepositorInsertLacZ
UseEntry vectorTagsHis and V5ExpressionMammalianMutationPromoterAvailable SinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMADM-beta
Plasmid#36891DepositorInsertsBeta-Geo
thymidine kinase
UseTagsExpressionMammalianMutationPromoterCAG (chicken beta actin promoter and CMV enhancer…Available SinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
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UAS:Zebrabow-V
Plasmid#118218PurposeFor broad or variegated gal4 inducible expression of Brainbow in zebrafishDepositorInsertBrainbow 1.0 L
UseZebrafish expressionTagsExpressionMutationPromoterAvailable SinceNov. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-nlsGFP-P2A
Plasmid#80808Purposenuclear localization signal fused to N-terminus of GFP (nlsGFP) without stop codon, followed by P2ADepositorInsertminimal cytomegalovirus immediate early enhancer/promoter
UseTagsExpressionMutationPromoterAvailable SinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNC1125
Plasmid#41561DepositorInsertUra3-GAL1-UBIMdkGFP*-SpHis5-Tim9
UseTagsExpressionMutationGFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…PromoterAvailable SinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFC331
Plasmid#87843PurposeAMA1 plasmid with Aspergillus optimized Cas9 and argB selection markerDepositorInsertsCas9
argB
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and native pro…Available SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAWH
Plasmid#133894PurposeExpression of Gal4AD-Prey hybrid protein. Homology regions for recombination with pBKWH, pBSWHhc or pMS22HDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable SinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBKWH
Plasmid#133898PurposeExpression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWHDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable SinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJOG641
Plasmid#105427PurposeSynthetic biologyDepositorInsertGAL4-VP16-GR (transcription factor: inducible expression; dexamethasone)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTEI042
Plasmid#105351PurposeSynthetic biologyDepositorInsertGal4UAS & 35S min promoter
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSCL.028
Plasmid#184965PurposeTest effect of extending -Eco1 a1/a2 in yeastDepositorInsertEco1: RT and ncRNA(extended), a1/a2 length: 27
UseTagsExpressionYeastMutationhuman codon optimized RT, a1/a2 length extended t…PromoterGal7Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pY2
Plasmid#218115PurposeActivity reporter backbone with an empty protease cassette (BsaI) under (lexA-box)3PminCYC1 promoter and an empty substrate cassette (BsmBI) under pGAL1 promoter.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJYDN3p
Plasmid#162457PurposePositive control plasmid for pJYDN3n plasmid expression attempts. It contains both eUnaG2 bilirubin dependent fluorescent protein and ALFA-tag for DnbALFA based detection on the cell surface.DepositorInsertAga2p-eUnaG2 (AGA2 )
UseShuttle vector bacteria/yeastTagsALFA tag, HA and myc tags, and eUnaG2 fluorescent…ExpressionMutationPromoterGAL1Available SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUAST cyto roGFP2-Orp1
Plasmid#64996Purposefor expression of cytosolic roGFP2-Orp1 with the Gal4 expression system in drosophilaDepositorAvailable SinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGRCRE
Plasmid#129116PurposeExpresses cre recombinase in Z. rouxii yeast cellsDepositorInsertcre
UseCre/LoxTagsExpressionYeastMutationPromoterS. cerevisiae GAL1 promoterAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUAS-hActin-luc2
Plasmid#48081PurposeLuciferase Reporter with Gal4 DNA binding site upstream of hACTB promoterDepositorInsertLuciferase
UseTagsExpressionMammalianMutationremoved hsp70 promoter, inserted amplified 615 bp…PromoterAvailable SinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
P303-FBP_2
Plasmid#139693PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, without recycling gene CPHDepositorInsertp35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:AtuOCS, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv-R896K::NG
Plasmid#138957PurposeUsed to generate a transgene that will express an inactive fluorescent Chitin Synthase in Drosophila using Gal4DepositorArticleInsertkkv-R896K::NG (kkv Fly)
UseTagsNeon Green fused to C terminus of KkvExpressionInsectMutationR896KPromoterUASAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-GYB
Plasmid#160927PurposeDual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (wild-type construct)DepositorInsertsBFP
sfGFP
UseTagsExpressionYeastMutationPromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMX-PGC1
Plasmid#6DepositorAvailable SinceJune 27, 2005AvailabilityAcademic Institutions and Nonprofits only -
pRS416-Aga1p-FAPB2.3.6
Plasmid#158147PurposeYeast display reporter for non-display yeast strains (wild-type construct)DepositorInsertAga1p-FAPB2.3.6
UseTagsExpressionYeastMutationPromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
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LV anti-FITC-LaG16-Y186F/Y229A-2Xmutant-T2A-BFP
Plasmid#194934PurposeLV construct encoding Y186F/Y229A double mutant sNRR-based SynNotch receptorDepositorInsertantiFITC-LaG16-sNRR(Y186F/Y229A)-SynNotch-Gal4-VP64-T2A-BFP
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGAD424-hSyntenin2b
Plasmid#89427PurposeTwo-hybrid systemDepositorAvailable SinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFGL1169R
Plasmid#116899PurposeCenpC:mCherry (centromere marker)DepositorInsertsCenp C (partial)
mCherry
CenpC downstream region
UseFungal expression (in magnaporthe oryzae)TagsExpressionMutationtruncated, no stop codon and without start codonPromoterAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLN429 (FuGW-G8p-mKate2)
Plasmid#105185PurposeModule 3 - GAL4 synthetic promoter ( with eight binding sites) drives mKate2 expressionDepositorInsertG8p-mKate2
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable SinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab4 DN
Plasmid#37691DepositorInsertRab4 (Rab4 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationS22N (dominant negative)PromoterGAL4Available SinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
HsCERK
Plasmid#118600PurposeExpresses ceramide kinase, which catalyzes the phosphorylation of ceramide to form ceramide 1-phosphateDepositorInsertHomo sapiens ceramide kinase
UseTagsFLAGExpressionYeastMutationPromoterGAL1, 10Available SinceFeb. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFGL1079
Plasmid#116897PurposeCenpC:eGFP (centromere marker)DepositorInsertsCenp C (partial)
eGFP
CenpC downstream region
UseFungal expression (in magnaporthe oryzae)TagsExpressionMutationtruncated, no stop codon and without start codonPromoterAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET5
Plasmid#107437PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMET5 (MET5 Budding Yeast)
UseTagsV5-HisExpressionYeastMutationV81I, K341E, P597L, Y605C, E741G, D932G, N1217S, …PromoterGAL1Available SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only