We narrowed to 22,805 results for: crispr

1. pHEE401E

   Plasmid

   #71287

   Purpose

   Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance

   Depositor

   Qi-Jun Chen

   Article

   Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

   Inserts

   gRNA scaffold
   zCas9
   

   Use

   CRISPR; Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Zea mays codon-optimized Cas9

   Promoter

   EC1.2 enhancer fused to EC1.1 promoter and U6-26p…

   Available since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHEE401

   Plasmid

   #71286

   Purpose

   Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance

   Depositor

   Qi-Jun Chen

   Article

   Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

   Inserts

   sgRNA scaffold
   zCas9
   

   Use

   CRISPR; Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Zea mays codon-optimized Cas9

   Promoter

   EC1.2 promoter and U6-26p Arabidopsis U6 gene pro…

   Available since

   Jan. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cas9/pTREX-n

   Plasmid

   #68708

   Purpose

   Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.

   Depositor

   Roberto Docampo

   Article

   Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

   Insert

   Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
   

   Use

   CRISPR

   Tags

   Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …

   Expression

   Mutation

   C4239T mutation that eliminates a BamHI restricti…

   Promoter

   Available since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHEE2E-TRI

   Plasmid

   #71288

   Purpose

   Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC

   Depositor

   Qi-Jun Chen

   Article

   Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

   Inserts

   sgRNA targeting TRY and CPC genes
   sgRNA targeting ETC2 gene
   zCas9
   

   Use

   CRISPR; Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Zea mays codon-optimized Cas9

   Promoter

   EC1.2 enhancer fused to EC1.1 promoter and U6-26p…

   Available since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Sox2-CreERT2

   Plasmid

   #135618

   Purpose

   Donor vector for genomic targeting of a P2A-CreERT2 cassette to the 3' end of the mouse Sox2 locus

   Depositor

   Lin He

   Article

   Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

   Insert

   P2A-CreERT2 flanked by Sox2 homology arms (Sox2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   P2A-CreERT2

   Expression

   Mutation

   Promoter

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pdCas9-DNMT3A-PuroR_hNT

   Plasmid

   #71830

   Purpose

   Expression of dCas9-DNMT3A fusion with T2A-PuroR and non-targeting control sgRNA for use in human cells

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   Non-targeting sgRNA human (DNMT3A Synthetic, Human, S. pyogenes)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-PuroR

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9

   Promoter

   U6

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

   Plasmid

   #64218

   Purpose

   Expression vector for sgRNA and for Expression of Cas9 linked to BFP via T2A linked to Ad4 E1B via P2A

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-BFP-P2A-E1B

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6

   Plasmid

   #64222

   Purpose

   Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry linked to the Ad4 E4orf6 gene via P2A

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-mCherry-P2A-E4orf6

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHL-H1-ccdB-mEF1a-RiH

   Plasmid

   #60601

   Purpose

   Cloning vector for CRISPR-sgRNA (into the BamHI-EcoRI site), expresses RFP and hygromycin resistance gene.

   Depositor

   Akitsu Hotta

   Article

   Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

   Inserts

   Red Fluorescent Protein
   Hygromycin resistance gene
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pS34.EF1α<GFP-2A-Cas9-RC

    Plasmid

    #207404

    Purpose

    Cas9-RC and GFP from an EF1α promoter. High-performance knockin through DSB repair by homology-directed recombination (HDR) or homology-mediated end joining (HMEJ). [Lab plasmid ID: N213]

    Depositor

    Alexandros Poulopoulos

    Article

    Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

    Insert

    Cas9-RC
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GFP-2A

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E4orf6

    Plasmid

    #64220

    Purpose

    Expression vector for sgRNA and Cas9 linked via T2A to BFP linked to the Ad4 E4orf6 gene via P2A

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA cassette
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-BFP-P2A-E4orf6

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

    Plasmid

    #64219

    Purpose

    Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked via T2A to BFP linked to the Ad4 E1B55K gene via P2A

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA targeting ROSA26-1
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-BFP-P2A-E1B

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHL-EF1a-SphcCas9-iP-A

    Plasmid

    #60599

    Purpose

    Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

    Depositor

    Akitsu Hotta

    Article

    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

    Insert

    CRISPR Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Codon usage optimized for human usage

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI)

    Plasmid

    #64217

    Purpose

    Expression vector for sgRNAs cloned into the BbsI sites, shRNAs into BamHI & AflII and for Expression of Cas9 linked to mCherry via T2A

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    hU6 promoter; BbsI sites for sgRNA
    H1 promoter; BamHI site for shRNA
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CBh, H1, and U6

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ262-ABE8e

    Plasmid

    #161523

    Purpose

    Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e mediated A-G base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

    Insert

    ABE8e-zSpCas9(D10A)
    

    Use

    CRISPR; Gateway compatible abe8e-zspcas9(d10a) en…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQ266E

    Plasmid

    #161521

    Purpose

    Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 PmCDA1 mediated C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

    Insert

    zSpRYCas9(D10A)-PmCDA1-UGI
    

    Use

    CRISPR; Gateway compatible zsprycas9(d10a)-pmcda1…

    Tags

    3X FLAG, NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ262B-ABE8e

    Plasmid

    #161524

    Purpose

    Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 ABE8e mediated A-G base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

    Insert

    TadA8e-zSpRYCas9(D10A)
    

    Use

    CRISPR; Gateway compatible tada8e-zsprycas9(d10a)…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ262m

    Plasmid

    #161522

    Purpose

    Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE7.10 mediated A-G base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

    Insert

    TadA(wt)-TadA(7.10)-zSpCas9(D10A)
    

    Use

    CRISPR; Gateway compatible tada(wt)-tada(7.10)-zs…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDF-casBCDE(g8)

    Plasmid

    #89727

    Purpose

    Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing 33-nt spacer (referred to as 'wt') matching a protospacer in the M13 phage genome.

    Depositor

    Konstantin Kuznedelov, Konstantin Severinov

    Article

    Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

    Insert

    CRISPR array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV Intein-C-SpyCas9, CMV-AcrIIA4-2xmiR-122 target sites

    Plasmid

    #120295

    Purpose

    AAV Vector for expression of C-terminal SpyCas9 fragement with split-intein and a CMV-driven AcrIIA4 with two miR-122 binding sites

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    C-terminal fragment of SpyCas9
    

    Use

    AAV and CRISPR

    Tags

    split-intein

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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