We narrowed to 5,415 results for: PEP;

1. pALPS tat-P2A-rev

   Plasmid

   #101331

   Purpose

   Lentivector expressing codon optimized NL4-3 tat and rev linked by P2A peptide coding sequence (GSGATNFSLLKQAGDVEENPGP)

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3 tat and rev
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGG-PM-TurboID

   Plasmid

   #209395

   Purpose

   Transiently expressing TurboID fused with a plasma menbrane targeting peptide in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Insert

   PM-3XHA-TurbOID
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Cauliflower mosaic virus (CaMV) 35S promoter

   Available since

   Jan. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMTTH+mEGFP-LANA

   Plasmid

   #157801

   Purpose

   Expression of mEGFP and LANA peptide fusion protein

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   mEGFP-LANA
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   pTAC

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+iii)

   Plasmid

   #192124

   Purpose

   pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+iii)

   Depositor

   Wilson Wong

   Article

   Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

   Insert

   pEF1a-anti-CD19scFV_CD28__41BB_peptide_P2A_DAP10_NS3(s139a)_CD3z-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQE80L TriCatcher-RGDSP

   Plasmid

   #112631

   Purpose

   Two SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site and a C-terminal SnoopCatcher

   Depositor

   Mark Howarth

   Article

   Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

   Insert

   TriCatcher-RGDSP
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   central RGDSP integrin-binding sequence

   Promoter

   T5

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. rat APOBEC1

   Plasmid

   #112858

   Purpose

   plasmid for expression of rat APOBEC1 in mammalian cells

   Depositor

   Silvestro Conticello

   Article

   Severi et al RNA Biol. 2015;12(4):389-97. doi: 10.108

   Insert

   APOBEC1 (Apobec1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Oct. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR1A_no_ccDB_mUty

   Plasmid

   #158570

   Purpose

   V5-tagged sequence of mUty cloned into entry vector for recombination

   Depositor

   Christian Deschepper

   Article

   Deschepper et al Sci Rep. 2020 Sep 10;10(1):14900. doi: 1

   Insert

   Mus musculus ubiquitously transcribed tetratricopeptide repeat containing, Y-linked (Uty), transcript variant 2 (Uty Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOCC114

   Plasmid

   #118904

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal sortase site

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-TEV-GG-NotI-ccdB-AscI-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with TEV protease, two glycines

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA-hDAGLb-V5

   Plasmid

   #87675

   Purpose

   Expresses human DAGLb protein with a HA tag inserted in the first extracellular loop, and intracellular V5 tag located on the C-terminal

   Depositor

   Pat Doherty

   Insert

   Human Diacylglycerol Lipase Beta (DAGLB Human)
   

   Use

   Tags

   HA and V5 tag

   Expression

   Mammalian

   Mutation

   Hemagglutinin (HA) peptide sequence (YPYDVPDYA) f…

   Promoter

   T7

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDS272

    Plasmid

    #34975

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2-GFP-LOVpep (MID2 Budding Yeast, A. sativa)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    T406A, T407A

    Promoter

    TEF

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMH-macroHC

    Plasmid

    #82337

    Purpose

    Provides a C-terminal macro domain and 6xHis tag for expression in E. coli

    Depositor

    Michael Hothorn

    Article

    Wild et al Protein Sci. 2016 Oct 24. doi: 10.1002/p

    Insert

    SPX domain of the Vacuolar Transporter Chaperone 4 (VTC4 Budding Yeast)
    

    Use

    Tags

    6xHis non-cleavable and macro domain of human his…

    Expression

    Bacterial

    Mutation

    Truncated protein, amino acids 1-178

    Promoter

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. human b1 ecto-pIRES

    Plasmid

    #113387

    Purpose

    Express human beta 1 ectodomain with secretion peptide, purification tags, and C-terminal clasp

    Depositor

    Timothy Springer

    Article

    Li et al EMBO J. 2017 Mar 1;36(5):629-645. doi: 1

    Insert

    human beta 1 ectodomain
    

    Use

    Tags

    His tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCLC-mEos2

    Plasmid

    #38010

    Depositor

    Xiaowei Zhuang

    Article

    Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

    Insert

    Clathrin, light polypeptide (LCa) (Clta Mouse)
    

    Use

    Tags

    mEos2

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pStr-KDEL_EPV-SBP-DsRed2-ER-5

    Plasmid

    #170676

    Purpose

    Expresses ER-targeted streptavidin-KDEL (hook) and SBP-DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide (cargo) for RUSH.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Inserts

    EPV-SBP-DsRed2-ER-5
    streptavidin-KDEL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    IRES and T7

    Available since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVH72-Tier1-PhCMV-SEAP

    Plasmid

    #169560

    Purpose

    Tier-1 vector encoding PhCMV-driven SEAP expression containing a computationally optimized secretion peptide (PhCMV-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven human secreted placental alkaline phosphatase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. fADL-1e-flag-linker-p3

    Plasmid

    #139441

    Purpose

    For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage.

    Depositor

    Alexander Gabibov

    Article

    Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

    Insert

    linker with pelB-leader, cloning site, flag-tag and p3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDS271

    Plasmid

    #34974

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2-GFP-LOVpep (MID2 Budding Yeast, A. sativa)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    T406A, T407A, V416I

    Promoter

    TEF

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDH_ss-SBP-EGFP

    Plasmid

    #172358

    Purpose

    Reporter to monitoring a streptavidin-binding peptide trafficking

    Depositor

    Guido Kroemer

    Article

    Zhao et al Sci Rep. 2018 Oct 8;8(1):14966. doi: 10.

    Insert

    ER importing signal sequence
    

    Use

    Lentiviral

    Tags

    SBP-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. fADL-1e-HA-linker-p3

    Plasmid

    #139440

    Purpose

    For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage

    Depositor

    Alexander Gabibov

    Article

    Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

    Insert

    linker with pelB-leader, cloning site, HA-tag and p3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Myog'-2A-mCherryNLS-PGK-Purodtk

    Plasmid

    #69547

    Purpose

    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

    Depositor

    Charles Gersbach

    Inserts

    2A-mCherry-NLS
    Myogenin 5' homology arm (Myog Mouse)
    Myogenin 3' homology arm (Myog Mouse)
    Puro-delta-tk
    

    Use

    Mouse Targeting

    Tags

    2x nuclear localization signal and PVT1-2A "…

    Expression

    Mutation

    Silent V205V mutation in myogenin gene to prevent…

    Promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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