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We narrowed to 13,023 results for: Mpl

Showing: 3841 - 3860 of 13023 results

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pTS103 pHAGE2 CMVtetO2 MCS-22xGS-GFP

Plasmid

#199356

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCk2_spacer

Plasmid

#136072

Purpose

Use when making even level constructs (L2,...) if pCk2 position (position 2) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Odd spacer

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-C1-hAtg14

Plasmid

#24295

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

autophagy related genes 14 (ATG14 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 27, 2010

Availability

Academic Institutions and Nonprofits only

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pSplit-CO-hDib1

Plasmid

#58788

Purpose

Express LucC-hDib1 fusion protein in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

hDib1 (TXNL4A Human)

Use

Tags

Flag and Luciferase (394-550aa)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pBMN mCherry-TAX1BP1(ΔUBD)

Plasmid

#119689

Purpose

For mammalian expression of TAX1BP1 without its UBD

Depositor

Michael Lazarou

Article

Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

UBD deletion (1-755)

Promoter

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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F-Cdk8

Plasmid

#15366

Depositor

Joan Conaway, Ronald Conaway

Article

Conaway et al Trends Biochem Sci. 2005 May . 30(5):250

Insert

CDK8 (CDK8 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P26

Plasmid

#120037

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mAID-Clover

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mAID-Clover

Use

Pcr template

Tags

mAID-Clover

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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L1_KanR-Ck1

Plasmid

#136123

Purpose

L1 in position 1 (next to LB in L2 construct), for KanR

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpEF1a:nptII_3t35S

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCk1_spacer

Plasmid

#136071

Purpose

Use when making even level constructs (L2,...) if pCk1 position (position 1) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Odd spacer

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pHAGE-TO-Sp dCas9-mVenusN

Plasmid

#191396

Purpose

SpdCas9 fused to N-terminal part of mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

N-terminal part of split mVenus

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pGEM-T Easy-AtpU6-26

Plasmid

#160218

Purpose

AtpU6-26 Golden Gate level 0 piece to express gRNAs

Depositor

Matias Kirst

Article

Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

Insert

pAtU6-26 promoter

Use

Golden gate level 0 piece to express grnas

Tags

Expression

Mutation

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA Sam68 deltaKH

Plasmid

#17688

Depositor

David Shalloway

Article

Lin et al J Biol Chem. 1997 Oct 24. 272(43):27274-

Insert

Sam68 (Khdrbs1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

aa 157-256 deleted (KH domain)

Promoter

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
YSBE 508 : pNEO_GFP

Plasmid

#92081

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; GFP tag, Neo selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

GFP

Use

tagging proteins in cryptococcus neoforman

Tags

Expression

Mutation

Promoter

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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modRNAc0-Cas9

Plasmid

#170180

Purpose

IVT template plasmid for making modified Cas9 RNA

Depositor

Xiaojun Lian

Article

Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

Insert

Cas9

Use

Ivt template plasmid

Tags

Expression

Mutation

Promoter

T7 promoter

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1X-Nup88

Plasmid

#87338

Purpose

To express human Nup88 in mammalian cells

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP88 (NUP88 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pSPlit-OC-hDib1

Plasmid

#58789

Purpose

Express hDib1-LucC fusion protein in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

hDib1 (TXNL4A Human)

Use

Tags

Flag and Luciferase (394-550aa)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3 Flag-MBD1-14-mVenusC

Plasmid

#191393

Purpose

MBD domain of MBD1 fused to C-terminal part of split mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

pcDNA3 Flag-MBD1-14-mVenusC

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV-EF1a-BFP-W

Plasmid

#208757

Purpose

Lentiviral vector expressing BFP

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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LAMP1-ABKAR

Plasmid

#65068

Purpose

Expression of ABKAR biosensor targeted to the lysozome

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

ABKAR biosensor

Use

Tags

Lamp1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only
Nuc-ABKAR

Plasmid

#61511

Purpose

Expression of nuclear AMPK biosensor

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Use

Tags

NLS (see comments)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only