We narrowed to 34,848 results for: CaS;

1. U6-CjCas9-MS2gRNA-Backbone

   Plasmid

   #210711

   Purpose

   This plasmid express MS2 loop containing Cj Cas9 gRNA

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   MS2 loop containing Cj Cas9 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-spCas9-N57

   Plasmid

   #169920

   Purpose

   Expression of SpCas9-N57 fusion protein to enhace site-specific DNA integration.

   Depositor

   Zhili Rong

   Article

   Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105

   Insert

   SpCas9-N57
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   N57 is the PAI domain of SB100X

   Promoter

   CAG

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. P823_eSpCas9(1.1)-RecA

   Plasmid

   #87264

   Purpose

   Human codon optimized RecA protein was fused to eSpCas9(1.1) for enhanced genome editing efficiency

   Depositor

   Yonglun Luo

   Article

   Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

   Insert

   RecA
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. eSpCas9(1.1)_No_FLAG

   Plasmid

   #79877

   Purpose

   FLAGless construct expressing high specificity eSpCas9(1.1). Px330-like plasmid.

   Depositor

   Yannick Doyon

   Article

   Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Untagged eSpCas9(1.1)

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. G1397 DddAtox-N–dSpCas9

   Plasmid

   #157835

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   bpNLS–G1397 DddAtox-N–dSpCas9–UGI–UGI–bpNLS
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-Flag-deSpCas9

   Plasmid

   #92114

   Purpose

   Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive eSpCas9 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A, K848A, K1003A, R1060A

   Promoter

   Cbh

   Available Since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpCas9 TadCBEa-V106W

   Plasmid

   #193838

   Purpose

   Express TadCBEa-V106W (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadA-CDa V106W-SpCas9 D10A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. RCAS(A) mLef1 (CT#1141)

   Plasmid

   #14021

   Depositor

   Cliff Tabin

   Insert

   Lef1 (Lef1 Mouse)
   

   Use

   Retroviral; Avian expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 16, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pNS38-SadCas9-SNAP

   Plasmid

   #113718

   Purpose

   Bacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9

   Depositor

   Gerald Schwank

   Article

   Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   HA-2xNLS-SNAP-NLS and His6-MBP-TEV

   Expression

   Bacterial

   Mutation

   D10A, N580A

   Promoter

   Available Since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-SUMO-AsCas12f

    Plasmid

    #171612

    Purpose

    AsCas12f1 heterologous expression plasmid

    Depositor

    Quanjiang Ji

    Article

    Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

    Insert

    AsCas12f1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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