We narrowed to 21,470 results for: SPR

Showing: 7721 - 7760 of 21470 results

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pMOD_C2806

Plasmid

#91091

Purpose

Module C, Promoter: FMV 34S, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4 (+ BaeI site for GT donor cloning)

Use

CRISPR

Promoter

FMV 34S

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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PCE(cm24Cre)

Plasmid

#242425

Purpose

for chromosome editing

Depositor

Caixia Gao

Article

Sun et al Cell. 2025 Aug 1:S0092-8674(25)00800-1.

Insert

PE, cm24Cre

Use

CRISPR

Expression

Plant

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-enCjCas9-BlastR

Plasmid

#220978

Purpose

Expresses human codon-optimized enCjCas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

enCjCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-3xGFP-tDeg

Plasmid

#191371

Purpose

Overexpression of 3xGFP-tDeg in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

3xGFP-tDeg

Tags

3xGFP

Expression

Mammalian

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-Halo-tDeg

Plasmid

#191370

Purpose

Overexpression of Halo-tDeg in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

Halo-tDeg

Tags

Halo

Expression

Mammalian

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pXZX353

Plasmid

#169457

Purpose

MATα (alpha) fragment in pTOPO vector

Depositor

Jef Boeke

Article

Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

Insert

MatA

Expression

Bacterial

Promoter

lac

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pJW1659

Plasmid

#154314

Purpose

NT Donor-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::AID*::3xFLAG for NT slot

Use

CRISPR

Expression

Worm

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB8748

Plasmid

#126918

Purpose

Plasmid containing integration cassette at IntE1 integration site in Yarrowia lipolytica for overexpression of XdCrtE

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtE

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB8739

Plasmid

#126905

Purpose

Template for PCR amplification of XdCrtYB

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtYB

Use

Synthetic Biology

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB8380

Plasmid

#126914

Purpose

Plasmid containing integration cassette at XII-5 integration site in Saccharomyces cerevisiae for overexpression of XdCrtE

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtE

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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library backbone 3'LTR gRNA and barcode

Plasmid

#127397

Purpose

Digestion by BstXI and BamHI to release the backbone

Depositor

Xiaowei Zhuang

Article

Wang et al Proc Natl Acad Sci U S A. 2019 May 28;11

Type

Empty backbone

Use

Lentiviral

Tags

Puro-mCherry

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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S12.2_Bridge + Target

Plasmid

#127671

Purpose

Ampicillin resistant multiplexing backbone with CRY2HA and mCherry

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Type

Empty backbone

Use

CRISPR

Tags

HA

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCR1446

Plasmid

#111129

Purpose

Expresses FANCF

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCF (FANCF Human)

Use

Lentiviral

Tags

HA

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pJG488

Plasmid

#91196

Purpose

WDV replicon T-DNA for gene targeting in wheat scutella, H840A double nickase (nTaCas9_H840A+gUbi8+gUbi1+donor)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

nTaCas9_H840A+gUbi8+gUbi1+donor

Use

CRISPR

Expression

Plant

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pLK88 - Edit-directing plasmid base

Plasmid

#98814

Purpose

Edit-directing plasmid base

Depositor

Leonid Kruglyak

Insert

CAN1.y gRNA

Use

CRISPR

Expression

Yeast

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_B2517

Plasmid

#91074

Purpose

Module B, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Esp3I ccdb cassette for single gRNA spacer cloning

Use

CRISPR

Promoter

TaU3

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTC364

Plasmid

#91213

Purpose

protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, Csy4 processing)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

2 gRNAs targeting tomato ARF8A

Use

CRISPR

Expression

Plant

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A1810

Plasmid

#91040

Purpose

Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_dead (D10A + H840A), Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-TaCas9_dead (D10A + H840A)

Use

CRISPR

Mutation

D10A, H840A

Promoter

ZmUbi

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only
pMOD_A0801

Plasmid

#91022

Purpose

Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9_dead (D10A + H840A)

Use

CRISPR

Mutation

D10A, H840A

Promoter

35S

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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ptreCas9-mKate2ps-T1gRNA

Plasmid

#62715

Purpose

Inducible; gRNA seq is ATGAGAATCAAGGCGGTCGA

Depositor

Leonidas Bleris

Article

Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

Insert

Cas9–mKate2ps

Expression

Mammalian

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pAP27

Plasmid

#194706

Purpose

Linker::3xFlag::mNeonGreen::HAtag::Linker

Depositor

Alexandre Paix

Article

Seleit et al Elife. 2021 Dec 6;10. pii: 75050. doi: 1

Insert

Linker::3xFlag::mNeonGreen::HAtag::Linker

Use

Synthetic Biology

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-i53

Plasmid

#119002

Purpose

Mammalian expression of i53 (53BP1) inhibitor and BFP

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

i53

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pYUU

Plasmid

#159751

Purpose

Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.

Depositor

Wolfgang Lukowitz

Article

Angulo et al bioRxiv

Insert

gRNA scaffold

Use

CRISPR

Expression

Plant

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pTW195b63

Plasmid

#136931

Purpose

Directed evolution of SpCas11

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

gIII(N)-npuN

Use

CRISPR

Expression

Bacterial

Mutation

none

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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LRT2B-GO2

Plasmid

#136909

Purpose

Lentivirus expressing GO2 sgRNA

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

sgGO2

Use

Lentiviral

Mutation

WT

Promoter

hU6

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB8742

Plasmid

#126908

Purpose

Template for PCR amplification of pGPD1-pFBA1 double promoter biobrick for Yarrowia lipolytica

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

pGPD1 and pFBA1

Use

Synthetic Biology

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB8379

Plasmid

#126913

Purpose

Plasmid containing integration cassette at X-4 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI and XdCrtYB

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI and XdCrtYB

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pSCBE2

Plasmid

#133965

Purpose

Expresses BE2 CRISPR base editor and gRNA scaffold in Streptomyces

Depositor

Yuhui Sun

Article

Zhong et al bioRxiv 630137

Insert

BE2

Use

CRISPR and Synthetic Biology

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMH183

Plasmid

#122856

Purpose

MS2-modified guide RNA targeting the FT promoter with GreenGate D-E flanking sequences

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

MS2-modified sgRNA targeting the Arabidopsis FT promoter

Use

Golden gate compatible cloning vector

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z29

Plasmid

#120072

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Strep-II

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Strep-II

Use

Pcr template

Tags

Strep-II

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z12

Plasmid

#120055

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mScarlet

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mScarlet

Use

Pcr template

Tags

mScarlet

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z09

Plasmid

#120052

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mVenus

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mVenus

Use

Pcr template

Tags

mVenus

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P27

Plasmid

#120038

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 1xHA

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

1xHA

Use

Pcr template

Tags

1xHA

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P24

Plasmid

#120035

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with TAP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

TAP

Use

Pcr template

Tags

TAP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P17

Plasmid

#120028

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mEos4b

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mEos4b

Use

Pcr template

Tags

mEos4b

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P08

Plasmid

#120019

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mClover3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mClover3

Use

Pcr template

Tags

mClover3

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pFS_0143-pET30-Fusicatenibacter_saccharivorans

Plasmid

#116952

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with classic acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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VKG1-gRNA-pX330

Plasmid

#108671

Purpose

Cleavage of VKG1 sequence by CRISPR/Cas9

Depositor

Shigeo Sugano

Article

Sawatsubashi et al Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1

Insert

gRNA for VKG1 sequence

Use

CRISPR

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pAtCas9-D10A_2

Plasmid

#91160

Purpose

Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:npt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A (nickase)

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pME-Fv2E-flt4um200-ns

Plasmid

#67696

Purpose

middle entry plasmid with dimerizable extracellular domain fused to um200 mutant Danio Flt4 cytoplasmic domain

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

Fv2E-flt4um200

Tags

HA

Mutation

um200, no stop codon; N1056S

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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Showing: 7721 - 7760 of 21470 results

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We narrowed to 21,470 results for: SPR