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mCerulean3-Vinculin-23

Plasmid

#55453

Purpose

Localization: Focal Adhesions, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Vinculin (VCL Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
iBlueberry T2A mCherry

Plasmid

#72434

Purpose

Expressing of iBlueberry and mCherry in mammalian cells

Depositor

Xiaokun Shu

Article

Yu et al Protein Sci. 2016 Feb;25(2):308-15. doi:

Insert

iBlueberry T2A mCherry

Expression

Mammalian

Mutation

mutation E177Q in iBlueberry

Available Since

May 24, 2016

Availability

Academic Institutions and Nonprofits only
mCerulean3-SiT-N-15

Plasmid

#55445

Purpose

Localization: Golgi TGN, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

SiT (ST6GAL1 Human)

Tags

mCerulean3

Expression

Mammalian

Mutation

aa 1-45 of SiT

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
pCAGGS_2pabPAC

Plasmid

#158968

Purpose

2P-activatable bPAC for transient expression

Depositor

Michiyuki Matsuda

Article

Kinjo et al ACS Chem Biol. 2020 Oct 19. doi: 10.1021

Insert

2pabPAC

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pTorPE-G-GECO1.1

Plasmid

#32467

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.1

Use

Lab constructed

Tags

6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

Expression

Bacterial

Mutation

GCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pLIC.B3.TM0851

Plasmid

#11530

Depositor

Sung-Hou Kim

Article

Liu et al J Mol Biol. 2005 Jul 29. 350(5):987-96.

Insert

heat-inducible transcriptional repressor (TM0851 T. maritima)

Tags

HisTag, TEV cleavable

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

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pBAD-DiB2

Plasmid

#168801

Purpose

Fluorogen activating protein (FAP) DiB2

Depositor

Alexander Mishin

Article

Bozhanova et al Chem Sci. 2017 Oct 1;8(10):7138-7142. do

Insert

Fluorogen activating protein DiB2

Tags

His-tag

Expression

Bacterial

Promoter

araBAD

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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(366) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4, K852A)

Plasmid

#168189

Purpose

HA-tagged Ubc6E nanobody fused to an inactive truncated OGT of 4 TPRs with K852A mutation

Depositor

Christina Woo

Article

Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

Insert

HA-nUbc6E-(EAAAK)4-OGT(4,K852A)

Tags

HA-Tag

Expression

Mammalian

Promoter

T7/CMV

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pKI-cjVASA-tdTomato

Plasmid

#160872

Purpose

Marmoset Targeting

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Stem Cell Res. 2021 Mar 24;53:102308. do

Insert

tdTomato

Use

Marmoset targeting

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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274_pSKB3-Venus-LUMP

Plasmid

#65976

Purpose

Bacterial expression of Venus-LUMP fusion with thrombin cleavage site, a FRET-based thrombin sensor

Depositor

Gerard Marriott

Article

Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

Insert

LUMP

Tags

Venus and thrombin site (11-residue linker that h…

Expression

Bacterial

Promoter

T7

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Rab4a-7

Plasmid

#55443

Purpose

Localization: Ras GTPase, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Rab4a (RAB4A Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only

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XE152 GFP-XDpr-CS2+

Plasmid

#16770

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

GFP-XDpr (dact1-b Frog)

Use

Xenopus expression

Tags

gfp

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pCAGGs-Hoxd10

Plasmid

#45721

Depositor

Jeremy Dasen

Article

Dasen et al Cell. 2008 Jul 25;134(2):304-16. doi: 10

Insert

Hoxd10 (HOXD10 Chicken)

Promoter

CMV / b-actin

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only
mOrange2-Endo-14

Plasmid

#57955

Purpose

Localization: Endosomes, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Endo (RHOB Human)

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pAP1638

Plasmid

#137089

Purpose

Plasmid for PCR-generated donor DNA for rapid tagging of proteins with 4*(GFP11)

Depositor

Geraldine Seydoux

Article

Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

Insert

4*(GFP11)

Use

Pcr template for donor dna

Expression

Bacterial

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pZyxin-mMaple2

Plasmid

#101150

Purpose

Fluorescent protein for superresolution imaging and a focal adhesion protein for binding substrate

Depositor

Xiaowei Zhuang

Article

Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

Insert

human codon optimized mMaple2 fused to zyxin

Expression

Mammalian

Promoter

cmv

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAP1836

Plasmid

#137087

Purpose

Plasmid for PCR-generated donor DNA for rapid tagging of proteins with mCherry

Depositor

Geraldine Seydoux

Article

Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

Insert

mCherry

Use

Pcr template for donor dna

Expression

Bacterial

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pFLIPglu-3.2mDelta13

Plasmid

#12992

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPglu F16A S112A Delta13

Use

Plant nanosensor

Tags

ECFP and EYFP

Expression

Plant

Mutation

F16A, S112A in binding protein

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only
pEG BacMam N term myc eGFP 3C

Plasmid

#160682

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Tags

myc eGFP 3C

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS426-TUB1-internal6xHis

Plasmid

#60390

Purpose

Expression of yeast alpha tubulin (TUB1) - internal 6xHis using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB1 (TUB1 Budding Yeast)

Expression

Yeast

Mutation

internal 6xHis (see supplement figure 4)

Promoter

GAL

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only