We narrowed to 9,400 results for: Coli

1. pJB07

   Plasmid

   #190481

   Purpose

   Targeting plasmid for 2-plasmid C. difficile mutagenesis system

   Depositor

   Joseph Sorg

   Article

   Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

   Inserts

   Upstream & Downstream pyrE deletion region
   gRNA
   

   Use

   E. coli / b. subtilis - c. difficile shuttle vect…

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNH-NanoLuc

   Plasmid

   #173075

   Purpose

   Expression vector for E. coli producing target protein with N-terminal Nanoluciferase fusion

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. GST-mCh-FYVE

   Plasmid

   #192289

   Purpose

   Expresses GST- and mCherry-tagged HRS FYVE in e Coli

   Depositor

   James Hurley

   Article

   Jensen et al Sci Adv. 2022 Dec 14;8(50):eadd1436. doi

   Insert

   HRS FYVE domain
   

   Use

   Tags

   GST-TEV-mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCBFD2Bad

   Plasmid

   #53574

   Purpose

   Produces an active CBFD (carotenoid β-ring 4-dehydrogenase) enzyme in E. coli when expression is induced with arabinose

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

   Insert

   cbfd2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available Since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmMaple3-CAM

   Plasmid

   #101148

   Purpose

   Fluorescent protein for superresolution imaging

   Depositor

   Xiaowei Zhuang

   Article

   Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

   Insert

   E. coli codon optimized mMaple3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pP450cam

   Plasmid

   #85085

   Purpose

   Expresses P450cam (CYP101A1) in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   P450cam
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPdR

   Plasmid

   #85083

   Purpose

   Expresses putidaredoxin reductase in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   Putidaredoxin reductase
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET_BGL3

   Plasmid

   #101912

   Purpose

   Expresses a β-glucosidase gene from Rhodothermus marinus in E. coli under the control of the pET system.

   Depositor

   Steve Shih

   Article

   Husser et al ACS Synth Biol. 2018 Mar 16;7(3):933-944

   Insert

   β-glucosidase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7+LacO

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSEVA221TadA*-T7RNAP

   Plasmid

   #167980

   Purpose

   Expresses fusion protein TadA*-T7RNAP in E. coli

   Depositor

   Luis Ángel Fernández

   Article

   Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

   Insert

   TadA*-T7RNAP
   

   Use

   Tags

   T7 tag and T7RNAP

   Expression

   Bacterial

   Mutation

   Promoter

   TetR-Ptet

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pReg-THP4

    Plasmid

    #190671

    Purpose

    Expresses an evolved tetrahydropapaverine biosensor in E. coli

    Depositor

    Andrew Ellington

    Article

    d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

    Insert

    THP4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    J23103

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-6xHis-GFP(-30)

    Plasmid

    #199167

    Purpose

    used to express a supercharged GFP variant in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(-30)
    

    Use

    Tags

    MGHHHHHHGGA

    Expression

    Mutation

    T19D, K36E, N49E, K51E, K62E, R83D, K89D, N159D, …

    Promoter

    T7

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSF-1b-S2

    Plasmid

    #128590

    Purpose

    Expresses tagless S2 ribosomal protein in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    30S ribosomal subunit protein S2 (rpsB E.coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETNb-cALFA

    Plasmid

    #159989

    Purpose

    For the protein expression with ALFA tag at C-terminus in E. coli

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Use

    Tags

    HRV3C protease cleavage site-ALFA tag-8histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Beta-Lactamase

    Plasmid

    #62729

    Purpose

    Expresses Beta-Lactamase protein in bacterial cells

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    Beta-Lactamase
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Deletion of periplasmic signal domain

    Promoter

    T7

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-WIPI3

    Plasmid

    #42527

    Depositor

    James Hurley

    Article

    Baskaran et al Mol Cell. 2012 Aug 10;47(3):339-48. doi:

    Insert

    WIPI3 (WDR45B Human)
    

    Use

    Tags

    GST-TEV

    Expression

    Mammalian

    Mutation

    gene sequence is codon optimized for expression i…

    Promoter

    Available Since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pHBFD1Bad

    Plasmid

    #53564

    Purpose

    Produces an active HBFD (carotenoid 4-hydroxy-β-ring 4-dehydrogenase) enzyme in E. coli when expression is induced with arabinose

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

    Insert

    hbfd1 cDNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJK226

    Plasmid

    #71125

    Purpose

    Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli

    Depositor

    T. J. Kappock

    Article

    Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

    Insert

    citrate synthase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. APRT

    Plasmid

    #64028

    Purpose

    Expression of S. crervisiae strain APRTase in E. coli. Contains N-terminal HistidineX6 tag.

    Depositor

    Vern Schramm

    Article

    Shi et al Biochemistry. 2001 Sep 11;40(36):10800-9

    Insert

    adenine phosphoribosyltransferase (APT1 Budding Yeast)
    

    Use

    Tags

    Histidine x 6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pJBM3

    Plasmid

    #225290

    Purpose

    E. coli-Campylobacter shuttle vector; KanR

    Depositor

    Robert Moore

    Article

    McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

    Insert

    Replication elements from pCC388 for pJBM3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEXP5-NT/pT7 LasI

    Plasmid

    #193631

    Purpose

    Constitutive T7 RNAP-mediated expression of LasI 3OC12-HSL synthase for Las quorum sensing system. LasI sequence is codon optimized for E. coli.

    Depositor

    Dora Tang

    Article

    Gonzales et al ChemSystemsChem 2023

    Insert

    LasI
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 RNAP promoter

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pEXP5-NT/pT7 LuxI

    Plasmid

    #193626

    Purpose

    Constitutive T7 RNAP-mediated expression of LuxI 3OC6-HSL synthase for Lux quorum sensing system. LuxI sequence is codon optimized for E. coli.

    Depositor

    Dora Tang

    Article

    Gonzales et al ChemSystemsChem 2023

    Insert

    LuxI
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 RNAP promoter

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pNIC-AncCAR_A

    Plasmid

    #168286

    Purpose

    Expresses AncCAR_A in E. coli from a T7 promoter

    Depositor

    Nicholas Harmer

    Article

    Thomas et al Commun Biol. 2019 Nov 22;2:429. doi: 10.

    Insert

    AncCAR_A
    

    Use

    Synthetic Biology

    Tags

    6*His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. pET-6xHis-GFP(+12)

    Plasmid

    #199161

    Purpose

    used to express a supercharged GFP variant in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(+12)
    

    Use

    Tags

    MGHHHHHHGGA

    Expression

    Mutation

    E16R, E44K, D86K, D112K, T115K, D127R, I138R, E15…

    Promoter

    T7

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pKM197

    Plasmid

    #132665

    Purpose

    pyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.

    Depositor

    Joseph Sorg

    Article

    Bhattacharjee et al mSphere. 2020 Mar 4;5(2):e00941-19. doi:

    Insert

    Upstream & Downstream pyrE deletion region
    

    Use

    E. coli - c. difficile shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. pRPF144

    Plasmid

    #106372

    Purpose

    C. difficile constitutive expression system. Shuttle plasmid containing constitutive Pcwp2 -gusA

    Depositor

    Neil Fairweather, Robert Fagan

    Article

    Fagan et al J Biol Chem. 2011 Aug 5;286(31):27483-93

    Insert

    Pcwp2-gusA (gusA )
    

    Use

    E. coli - c. difficile shuttle vector

    Tags

    Expression

    Mutation

    codon optimised for C. difficile

    Promoter

    Pcwp2

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCN38-yfp

    Plasmid

    #227637

    Purpose

    Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

    Depositor

    Angelika Grundling

    Article

    Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

    Insert

    yfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET-6xHis-GFP(+18)

    Plasmid

    #199162

    Purpose

    used to express a supercharged GFP variant in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(+18)
    

    Use

    Tags

    MGHHHHHHGGA

    Expression

    Mutation

    E16R, D29K, E42K, E44K, D86K, D112K, T115K, D127R…

    Promoter

    T7

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TARG1-c002

    Plasmid

    #173108

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    TARG1
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    Full length protein

    Promoter

    Available Since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28a-RILP

    Plasmid

    #74600

    Purpose

    used for expression in E. coli and purification

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    Rab-interacting lysosomal protein (RILP Human)
    

    Use

    Tags

    FLAG, StrepII, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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30. enz.083-NMT1

    Plasmid

    #154047

    Purpose

    Expresses NMT1 in E. coli

    Depositor

    Christopher Voigt

    Article

    Wallace et al Advanced Functional Materials 2020

    Insert

    NMT1 (N-myristoyltransferase 1) (NMT1 Human)
    

    Use

    Tags

    His, MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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