We narrowed to 10,522 results for: SEC
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Plasmid#153329PurposeTriggers secretion of SptP120 fused to the K55 DARPin from Salmonella upon induction with arabinoseDepositorInsertSptP120-K55 DARPin
UseSalmonella expressionAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-21
Plasmid#172749PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-21; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-21
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-37
Plasmid#172756PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-37; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-37
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4B-Agam_695
Plasmid#176668PurposeExpression of sgRNA under An. gambiae U6-2 (AGAP013695) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprExpressionInsectAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-17
Plasmid#172747PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-17; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-17
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACE-GIRK4-S
Plasmid#172425PurposeExpression of full-length, human GIRK4 with C-terminal StrepTag IIDepositorAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUASt-GBP-Trbo
Plasmid#179996PurposeExpresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASt version is ideal for expression in somatic tissues.DepositorInsertGBP nanobody
TagsTrboExpressionInsectAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT dati
Plasmid#91800PurposeExpresses the Drosophila dati circular RNADepositorAvailable SinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac(His10-SNAP-Csk)
Plasmid#177895PurposeBaculo expression of His10 tag fused to SNAPtag and human CskDepositorAvailable SinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRKB134_GI-DUX4-intron2(-)
Plasmid#83968Purposeto test DUX4 mRNA is subjected to NMD upon intron2 inclusionDepositorAvailable SinceJan. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRKB133_GI-DUX4-intron2(+)
Plasmid#83967Purposeto test DUX4 mRNA is subjected to NMD upon intron2 inclusionDepositorAvailable SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
FLAG-mFzd4-ΔC(40-513)-3C-sGFP1-10_pVL1393
Plasmid#216380PurposeBaculovirus transfer vector to express FLAG-tagged mouse Fzd4 ΔC (residues 40-513) fused to split GFP (strands 1-10)DepositorInsertFrizzled-4 (Fzd4 Mouse)
UseBaculovirusTags3C-splitGFP1to10opt-ETVV and HA signal sequence-F…ExpressionInsectPromoterPolyhedrinAvailable SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIHEU_miniSOG2 T2A H3.3A-EGFP
Plasmid#87411PurposeUAS vector to express Histone 3.3A GFP fusion and miniSOG2 in DrosophilaDepositorInsertmini singlet oxygen generator 2
ExpressionInsectAvailable SinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-SMAD3
Plasmid#79507PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human SMAD3 CDSDepositorAvailable SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSPYngK+-hp
Plasmid#48121PurposeShuttle vector E. coli/B.meg.; carrying the codon optimized sequence of the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoterDepositorInsertcodon optimized signal peptide of YngK
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFasBac+GFP-CAMSAP3 CC+CKK
Plasmid#59041Purposefor baculovirus expression of mouse CAMSAP3 CC+CKK in Sf9 cellsDepositorAvailable SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-AthHEN1
Plasmid#104980PurposeExpression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cellsDepositorAvailable SinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4-Aalb_744
Plasmid#176663PurposeExpression of sgRNA under Ae. albopictus U6 (AALF029744) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprExpressionInsectAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTY153 EGFP-T2A-ORF6 SARS-CoV-2
Plasmid#204975PurposeCo-express EGFP and SARS-CoV-2 ORF6DepositorAvailable SinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTY152 EGFP-T2A-ORF6 SARS-CoV-1
Plasmid#204974PurposeCo-express EGFP and SARS-CoV-1 ORF6DepositorAvailable SinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCaSpeR4 mito roGFP2-Orp1
Plasmid#65001PurposeExpression of mitochondrial roGFP2-Orp1 under the alpha Tub84B promoterDepositorInsertOrp1 (HYR1 Budding Yeast)
TagsroGFP2ExpressionInsectMutationmitochondrial targeting sequenceAvailable SinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJFRC210-10XUAS-FRT>STOP>FRT-myr::smGFP-OLLAS
Plasmid#63170PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-OLLAS reporterDepositorInsert"spaghetti monster GFP-OLLAS"
TagsN-myristoylationExpressionInsectAvailable SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOEM-GED-EGFP
Plasmid#102782PurposeBaculovirus rescue vector, VSVGED pseudotype, eGFPDepositorInsertEGFP
UseBaculoviral rescue shuttle vectorExpressionInsect and MammalianPromoterCMVAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LINXb3
Plasmid#73615PurposeLight-induced nuclear export switch for microscopy in mammalian cells.DepositorInsertLINXb3
TagsmCherryExpressionBacterial, Insect, and Mamm…Available SinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
CapChR2_pUAST-attb
Plasmid#188041PurposeExpresses CapChR2 in DrosophilaDepositorInsertCapChR2
TagsnoneExpressionInsectMutationS43D, L112C, T139C and N238E mutations in CoChRPromoterUASAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(chimpanzee)
Plasmid#79032PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(chimpanzee)
TagsOneSTrEP-FLAGExpressionInsectPromoterpolyhedrinAvailable SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAC8-TwinStrep-3C-DrHDAC6 (40-831)
Plasmid#180226PurposeBaculovirus transfer plasmid to express TwinStrep-3C-DrHDAC6 (40-831)DepositorInsertHistone deacetylase 6 (hdac6 Zebrafish)
TagsTwinStrep-3CExpressionInsectPromoterpolyhedrinAvailable SinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
874V=βTub-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40
Plasmid#159672PurposePlasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.DepositorInsertβTub-NLS-hSpCas9-T2A-GFP
TagsT2A-GFPExpressionInsectAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4-Cquin_596
Plasmid#176655PurposeExpression of sgRNA under C. quinquefasciatus U6 (CPIJ039596) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprExpressionInsectAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSL1180-HR-PUbEGFP-NLS
Plasmid#176681PurposeExpression of nuclear localized EGFP under Ae. aegypti Polyubiquitin promoter (AAEL003888), EGFP version of pSL1180-HR-PUbECFP (Addgene #47917)DepositorInsertEGFP
ExpressionInsectPromoterAe. aegypti poly-ubiquitin (AAEL003888)Available SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pLpC-Reg-L-IFP-F[2]+Venus (mammalian)
Plasmid#52903PurposeBinary retroviral pl. (puromycin). Reg:Linker (GGGGS)X2+IFP-F[2] (PacI/EcoRI). In the second MCS, the Venus fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.DepositorInsertReg-L-IFP-F[2]+Venus
UseRetroviralTagsL-IFP-F[2]ExpressionMammalianPromoterUnknownAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLpC-Cat-L-IFP-F[1]+Plum (mammalian)
Plasmid#52905PurposeBinary retroviral pl. (puromycin). Cat(PacI/ClaI):Linker (GGGGS)X2+IFP-F[2]. In the second MCS, the Plum fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.DepositorInsertCat-L-IFP-F[1]+Plum
UseRetroviralTagsL-IFP-F[1]ExpressionMammalianPromoterUnknownAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASP dsRed Rab11 WT
Plasmid#44829DepositorAvailable SinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUAST mito roGFP2-Orp1
Plasmid#64997Purposefor expression of mitochondrial roGFP2-Orp1 with the Gal4 expression system in drosophilaDepositorInsertOrp1 (HYR1 Budding Yeast)
TagsroGFP2ExpressionInsectMutationmitochondrial targeting sequenceAvailable SinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCASP-SptP167-3G124-HilA
Plasmid#153322PurposeTriggers secretion of SptP167 fused to the 3G124nc anti-GFP DARPin from Salmonella upon induction with arabinoseDepositorInsertSptP167-3G124 DARPin
UseSalmonella expressionAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCASP-SptP120-TD-HilA
Plasmid#153330PurposeTriggers secretion of SptP120 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinoseDepositorInsertSptP120-HA4-7c12 Tandem Monobody
UseSalmonella expressionAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT-GFP-p150Dynactin
Plasmid#24283DepositorAvailable SinceJune 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-27
Plasmid#172752PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-27
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_mCherry::Nrv1::vhhGFP4
Plasmid#163926PurposeExpression of GrabFP-B-intracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (intracellular) nanobody with Nrv1 protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectAvailable SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only