We narrowed to 7,531 results for: Ski

1. SiC-V2-Cas9G7

   Plasmid

   #133043

   Purpose

   Doxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.

   Depositor

   Sriram Neelamegham

   Article

   Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

   Insert

   Tet repressor
   

   Use

   Lentiviral

   Tags

   Cerulean

   Available Since

   Nov. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 Ash2L full

   Plasmid

   #15548

   Depositor

   Kai Ge

   Article

   Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

   Insert

   Ash2L (ASH2L Human)
   

   Expression

   Mammalian

   Mutation

   R622H (Please see depositor comments below)

   Available Since

   Aug. 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAIP hIL2 co

   Plasmid

   #90513

   Purpose

   Lentiviral vector for expression of human codon optimized IL2 with puromycin resistance.

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   IL2 (IL2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-p54

   Plasmid

   #35379

   Depositor

   Benjamin Blencowe

   Article

   Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

   Insert

   p54 (NONO Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   A464V

   Promoter

   CMV

   Available Since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLIK-Rbfox2-rABE-Flag

   Plasmid

   #191384

   Purpose

   Doxycycline inducible expression of Rbfox2-rABE

   Depositor

   Stephen Floor

   Article

   Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

   Insert

   Rbfox2-rABE (RBFOX2 Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   Flag

   Promoter

   tet-inducible promoter

   Available Since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLYS6-METTL17-FLAG

   Plasmid

   #220875

   Purpose

   Expressing METTL17-FLAG

   Depositor

   Vamsi Mootha

   Article

   Ast et al Mol Cell. 2024 Jan 18;84(2):359-374.e8.

   Insert

   METTL17 (METTL17 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pInducer20 Cyclin E1

   Plasmid

   #109348

   Purpose

   virus packaged, doxycyline inducible expression of Cyclin E1

   Depositor

   Jean Cook

   Article

   Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

   Insert

   Cyclin E1 (CCNE1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCEFL EGFP-TEADi

   Plasmid

   #140144

   Purpose

   TEAD inhibitor (TEADi): GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal.

   Depositor

   Ramiro Iglesias-Bartolome

   Article

   Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

   Insert

   EGFP TEADi
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA5/FRT_CMV_NES-Caprola_04-mEGFP

   Plasmid

   #194682

   Purpose

   CMV driven expression of the calcium recorder Caprola_04 in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_04-mEGFP
   

   Tags

   mEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEND-320_Cacnes_ori

    Plasmid

    #225602

    Purpose

    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a low-copy pBR322 E. coli ori

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. Adeno EA

    Plasmid

    #64071

    Purpose

    Adenovirus for the expression of gRNAs targeting intron 19 of murine Alk and intron 14 of Eml4

    Depositor

    Andrea Ventura

    Article

    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

    Insert

    U6_sgRNA(Alk)_U6_sgRNA(Eml4)_CBh_FLAG-Cas9
    

    Use

    Adenoviral

    Tags

    FLAG-Cas9

    Promoter

    U6 and CBh

    Available Since

    July 23, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCMV4a-SIRT2-Flag

    Plasmid

    #102623

    Purpose

    Expresses full length SIRT2 isoform 1 in mammalian cells

    Depositor

    Hening Lin

    Article

    Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

    Insert

    Sirtuin 2 (SIRT2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA pDEST40 Cas9-POLD3

    Plasmid

    #183194

    Purpose

    Expression clone

    Depositor

    Emma Haapaniemi

    Article

    Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

    Insert

    POLD3
    

    Use

    CRISPR

    Tags

    Cas9WT

    Expression

    Mammalian

    Mutation

    n/a

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA5/FRT/TO_TOMM20_HaloTag9_T2A_EGFP

    Plasmid

    #169333

    Purpose

    Expression of HaloTag9 at the mitochondrial surface of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    TOMM20-HaloTag9 (TOMM20 Human)
    

    Tags

    HaloTag9

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA SF-HERC2 F6

    Plasmid

    #55789

    Purpose

    Expresses N-terminal 3xFLAG/STREP tagged HERC2 (4421-4834aa)

    Depositor

    David Chan

    Article

    Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

    Insert

    3xFLAG tagged HERC2 (4421-4834aa) (HERC2 Human)
    

    Tags

    3xFLAG and STREP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 24, 2014

    Availability

    Academic Institutions and Nonprofits only

16. His-MBP_G4G6 in pRSF

    Plasmid

    #188454

    Purpose

    Express in E. coli human gelsolin segments 4 through 6 with N-terminal 6xHis and MBP tags.

    Depositor

    Roberto Dominguez

    Article

    Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

    Insert

    G4G6 (GSN Human)
    

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hsyn-flex-dsRed-shvgat

    Plasmid

    #67845

    Purpose

    Expresses shRNA to the vesicular GABA transporter VGAT. Cre dependent expression by flex switch

    Depositor

    William Wisden

    Article

    Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

    Insert

    AAV-hsyn-flexed-dsRed-miR30-vgat2.shRNA
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and RNAi

    Expression

    Mammalian

    Promoter

    h-synapsin

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. p3HM1Luc4

    Plasmid

    #236277

    Purpose

    Mammalian expression of human muscarinic M1 receptor; N-terminal HA tag, C-terminal Rluc8

    Depositor

    Vsevolod Gurevich

    Article

    Gimenez et al J Biol Chem. 2012 Aug 24;287(35):29495-5

    Insert

    muscarinic M1 receptor (CHRM1 Human)
    

    Tags

    3xHA and Rluc8

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.1-puro_CMV_NES-Caprola_06-mEGFP

    Plasmid

    #194693

    Purpose

    CMV driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through through lentivirus transduction

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_06-mEGFP
    

    Use

    Lentiviral

    Tags

    mEGFP

    Promoter

    CMV

    Available Since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO.1-puro_CMV_NES-Caprola_off-mEGFP

    Plasmid

    #194695

    Purpose

    CMV driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through lentivirus transduction

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_off-mEGFP
    

    Use

    Lentiviral

    Tags

    mEGFP

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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