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pET_RTX_(exo-)

Plasmid

#102786

Purpose

Expresses the Reverse Transcriptase gene (3'-5' exonuclease deficient mutant)

Depositor

Andrew Ellington

Publication

Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

Insert

reverse transcription xenopolymerase with 3'-5' deficient exonuclease

Expression

Bacterial

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKM444

Plasmid

#108319

Purpose

For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase

Depositor

Kenan Murphy

Publication

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Inserts

Che9C RecT
Bxb1 Integrase
Tet Repressor

Expression

Bacterial

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pGL3 2 kb prom. CD274

Plasmid

#107003

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Publication

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Expression

Mammalian

Available since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAV.CAG.LSL.tdTomato

Plasmid

#100048

Purpose

AAV mediated Cre-dependent expression of tdtomato (Lox-Stop-Lox)

Depositor

Hongkui Zeng

Publication

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Insert

tdtomato

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pAW63.YY1.FKBP.knock-in.BFP

Plasmid

#104371

Purpose

For knocking in FKBP F36V P2A BFP

Depositor

Richard Young

Publication

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Insert

FKBP12(F36V) -P2A-BFP

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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-5.5kb-UCP1-GFP

Plasmid

#104585

Purpose

Reporter assay to monitor the transcription activity of the UCP1 promoter by GFP expression

Depositor

Irfan Lodhi

Publication

Lodhi et al Cell Rep. 2017 Sep 19;20(12):2766-2774.

Insert

UCP1 promoter (Ucp1 Mouse)

Tags

TurboGFP

Expression

Mammalian

Promoter

UCP1

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLJC5-LAMP1-RFP-2xFlag

Plasmid

#102931

Purpose

Lentiviral expression of a lysosomal tag (LAMP1-mRFP1-Flag)

Depositor

David Sabatini

Publication

Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

Insert

LAMP1

Use

Lentiviral

Tags

mRFP-2xFlag

Promoter

ubc

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSNAP-tag (T7) Vector

Plasmid

#101137

Purpose

Expression of protein of interest with SNAP-Tag in bacterial cells

Depositor

New England Biolabs, Ana Egana

Type

Empty backbone

Tags

SNAP-tag (SNAP26b)

Expression

Bacterial

Available since

Sept. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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B52 (empty plasmid backbone to express 2 sgRNAs)

Plasmid

#100708

Purpose

Empty plasmid backbone to express 2 sgRNAs (use BbsI and BsmBI for cloning)

Depositor

Alberto Ciccia

Publication

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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DNA-PKcs T3950D

Plasmid

#83320

Purpose

DNA-PKcs T3950A

Depositor

Katheryn Meek

Publication

Douglas et al Mol Cell Biol. 2007 Mar;27(5):1581-91. E

Insert

human DNA-PKcs (PRKDC Human)

Expression

Mammalian

Mutation

Activation loop phosphorylation site 3950 substit…

Available since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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MICU-APEX2

Plasmid

#79057

Purpose

A C-terminal APEX2 fusion to the human mitochondrial calcium uptake 1 protein MICU1

Depositor

Alice Ting

Publication

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

C-terminal APEX2 fused MICU1 (MICU1 Human, Soybean)

Use

Lentiviral

Tags

FLAG

Expression

Bacterial and Mammalian

Promoter

CMV immearly promotor

Available since

Sept. 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLH-sgRNA1-2XPP7

Plasmid

#75390

Purpose

sgRNA1-2XPP7

Depositor

Thoru Pederson

Publication

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1-2XPP7

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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AAV-mOXT-hM3Dq-mCherry-WPRE

Plasmid

#70717

Purpose

Expresses DREADDs Gq receptor under oxytocin promoter control

Depositor

Daniel Geschwind

Publication

Penagarikano et al Sci Transl Med. 2015 Jan 21;7(271):271ra

Insert

hM3Dq-mCherry

Use

AAV

Tags

mCherry

Promoter

mouse oxytocin

Available since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pRetroX-Tight-MCS_PGK-GpNLuc

Plasmid

#70185

Purpose

Constiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

Depositor

Antonio Amelio

Publication

Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

PGK

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLV-STAT2

Plasmid

#71451

Purpose

3rd generation lentiviral expression of STAT2

Depositor

George Stark

Publication

Cheon et al EMBO J. 2013 Oct 16;32(20):2751-63. doi:

Insert

STAT2 (STAT2 Human)

Use

Lentiviral

Tags

FLAG

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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CAG-FLEx(FRT)-TC

Plasmid

#67827

Purpose

Flp-dependent expression of TVA-mCherry fusion to mediate modifed rabies virus expression in cells

Depositor

Liqun Luo

Publication

Schwarz et al Nature. 2015 Aug 6;524(7563):88-92. doi:

Insert

TVA-mCherry fusion

Use

AAV

Expression

Mammalian

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pFRT-TODestGFP_RNAseH1

Plasmid

#65784

Purpose

expresses GFP-tagged RNAseH1 in mammalian cells

Depositor

Thomas Tuschl

Insert

RNaseH1 (RNASEH1 Zebrafish, Human)

Tags

GFP and V5

Expression

Mammalian

Promoter

CMV, 2x Tet

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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TRIM33-GFP

Plasmid

#65399

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Publication

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

TRIM33 (TRIM33 Human)

Tags

EmGFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHSN401

Plasmid

#50588

Purpose

CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

Depositor

Qi-Jun Chen

Publication

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Inserts

3×FLAG-NLS-zCas9-NLS
gRNA scaffold

Use

CRISPR; Plant expression

Tags

3xFLAG and NLS

Mutation

maize codon optimized

Promoter

2×35Sp and AtU6-26p

Available since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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mCardinal-pBAD

Plasmid

#54800

Purpose

Localization: Protein Expression Vector , Excitation: 604, Emission: 659

Depositor

Michael Davidson

Publication

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Type

Empty backbone

Tags

6xHis and mCardinal

Expression

Bacterial

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pMDIAI

Plasmid

#51655

Purpose

E coli genetic modification

Depositor

Sheng Yang

Publication

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

apramycin antibiotic resistance gene

Use

Genetic modification

Promoter

unknown

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pWUR702

Plasmid

#53079

Purpose

Inducible TtAgo expression

Depositor

John van der Oost

Publication

Swarts et al Nature. 2014 Mar 13;507(7491):258-61. do

Insert

TtAgo

Tags

Strep-II tag

Expression

Bacterial

Promoter

T7

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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DRH335: FCK-Optopatch1

Plasmid

#51695

Purpose

Coexpression plasmid of QuasAr1 and CheRiff for all-optical electrophysiology

Depositor

Adam Cohen

Publication

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

Optopatch1

Use

Lentiviral

Expression

Mammalian

Promoter

CaMKIIa

Available since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAluYa5-neo-TET

Plasmid

#51283

Purpose

expresses Alu, a human SINE, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette

Depositor

Astrid Roy-Engel

Publication

Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

Insert

AluYa5

Tags

neoTET cassette with a tetrahymena self-splicing …

Expression

Mammalian

Promoter

upstream pol III enhancer sequence of the 7SL gen…

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mCherry-FKBP-MTM1

Plasmid

#51614

Purpose

Recruitable PtdIns3P/PtdIns(3,5)P2 phosphatase

Depositor

Tamas Balla

Publication

Hammond et al J Cell Biol. 2014 Apr 7.

Inserts

FKBP
MTM1 (MTM1 Human, 1812)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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Syn-ATP

Plasmid

#51819

Purpose

Optical reporter of presynaptic ATP

Depositor

Timothy Ryan

Publication

Rangaraju et al Cell. 2014 Feb 13;156(4):825-35. doi: 10

Insert

Chimeric Synaptophysin-mCherry-Luciferase

Expression

Mammalian

Mutation

Within WT luciferase gene, changed Threonine 214 …

Promoter

CMV

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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M-NM-sgRNA

Plasmid

#48673

Purpose

Mammalian U6-driven sgRNA (NMm1) targeting GTCCCCTCCACCCCACAGTG

Depositor

George Church

Publication

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with N. meningitidis Cas9, hU6 promoter

Use

CRISPR

Promoter

hU6

Available since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pACYCT2

Plasmid

#45799

Depositor

Luc Ponchon

Publication

Ponchon et al Nucleic Acids Res. 2013 Aug;41(15):e150.

Type

Empty backbone

Expression

Bacterial

Available since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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EYFP-hCaM

Plasmid

#47603

Depositor

Emanuel Strehler

Publication

Rogers et al J Biol Chem. 2001 Apr 13;276(15):12182-9

Insert

CaM (CALM3 Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pBAD/HisB-iRFP670

Plasmid

#45453

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP670

Expression

Bacterial

Mutation

T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

Promoter

araBAD

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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